LOCUS       pHS-AVC-LW490           2451 bp    DNA     circular UNA 10-OCT-2017
DEFINITION  pHS-AVC-LW490, psb1c3-U6-sgRNA(BRAF,V600E)-sgRNA_backbone.
ACCESSION   urn.local...c9-85wa3lz
VERSION     urn.local...c9-85wa3lz
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      226..839
                     /created_by="User"
                     /label="rep(pMB1)"
     terminator      1007..1112
                     /created_by="User"
                     /label="T0 terminator"
     misc_difference complement(1126..1785)
                     /created_by="User"
                     /label="Chloramphenicol resistance"
     misc_feature    2036..2293
                     /label="Human U6 promoter"
                     /note="Geneious type: promoter uukaryotic"
     misc_RNA        2293..2312
                     /created_by="User"
                     /modified_by="User"
                     /label="sgRNA"
     misc_RNA        2313..2389
                     /created_by="User"
                     /label="sgRNA_backbone"
     terminator      2390..2396
                     /label="Pol III terminator"
ORIGIN      
        1 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
       61 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      241 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      661 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      901 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      961 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
     1021 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1081 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1141 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1201 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1261 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1321 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     1381 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1441 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1501 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1561 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1621 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1681 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1741 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     1801 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1861 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca
     1921 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
     1981 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagaggcag
     2041 gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     2101 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     2161 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     2221 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     2281 ggacgaaaca ccgtgatttt ggtctagcta cagttttaga gctagaaata gcaagttaaa
     2341 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttttaagc
     2401 ttggctccgg tgcccgtcac tagtagcggc cgctgcagtc cggcaaaaaa g
//
