LOCUS       piG17-2-002             3259 bp    DNA     circular UNA 01-NOV-2017
DEFINITION  wrong BB PF - 0.71 strength anderson promotor - RS1 (NcoI) - ~5000
            Salis RBS - TlpA CDS - SF.
ACCESSION   urn.local...hh-88wu383
VERSION     urn.local...hh-88wu383
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      7..101
                     /locus_tag="/product="
                     /label="lambda t0 terminator|terminator"
     CDS             252..911
                     /locus_tag="/product=""
                     /translation=""MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITA
                     F
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
                     /label="chloramphenicol_acetyltransferase(CAT)_ORF
                     Selectab le marker|CDS"
     misc_feature    925..1170
                     /locus_tag="/product="
                     /label="oriT|misc_feature"
     rep_origin      1215..1760
                     /locus_tag="/note=""Plasmids containing the
                     medium-copy-number p15A orig in of replicationcan be
                     propagated in E. colice llsthat contain a second plasmid
                     with the ColE1 origi n."
                     /product
                     /label="p15A ori"
     unsure          complement(1767..1770)
                     /locus_tag="/note=""CAAAA > CAAA in sequencing 14.11.2014"
                     /product
                     /label="CAAAA > CAAA in sequencing 14.11.2014"
     terminator      complement(1938..2024)
                     /locus_tag="/note=""transcription terminator T1 from the
                     E. coli rrnB ge ne"
                     /product
                     /label="rrnB T1 terminator"
     misc_feature    2031..2050
                     /created_by="nicolashuber"
                     /modified_by="nicolashuber"
                     /label="BioBrick Prefix 2"
     promoter        2051..2085
                     /created_by="lidavadakumchery"
                     /modified_by="lidavadakumchery"
                     /label="BBa_J23104"
     misc_feature    2057..2062
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="NheI"
                     /note="Geneious type: restriction site"
     misc_feature    2080..2085
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="NheI"
                     /note="Geneious type: restriction site"
     misc_feature    2086..2091
                     /created_by="irma"
                     /label="1-RS1 (NcoI) "
     misc_feature    2086..2122
                     /Original_Bases="GAGCTC"
                     /label="GAGCTC"
                     /note="Geneious type: Editing History Replacement"
     RBS             2092..2122
                     /created_by="nicolashuber"
                     /modified_by="nicolashuber"
                     /label="medium synthetic RBS for tlpA"
     CDS             2123..3238
                     /created_by="nicolashuber"
                     /label="tlpA-CDS"
     misc_feature    2176
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2179
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2416
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2533
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2632
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2692
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2746
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2905
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3239..3259
                     /created_by="nicolashuber"
                     /modified_by="nicolashuber"
                     /label="BioBrick Suffix"
ORIGIN      
        1 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
       61 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
      121 acgatttaaa ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
      181 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
      241 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      301 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      361 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      421 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa
      481 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      541 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
      601 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
      661 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
      721 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
      781 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
      841 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
      901 gcggggcgta atttgacttt tgtccttttc cgctgcataa ccctgcttcg gggtcattat
      961 agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt
     1021 tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg
     1081 cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa
     1141 cgggaatcct gctctgcgag gctggccgta ggccggccct agaaatattt tatctgatta
     1201 ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa
     1261 aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc tttgaaccga
     1321 ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta gccttaaccg
     1381 gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccagtggtgc
     1441 ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1501 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact
     1561 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
     1621 gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc tggtatcttt
     1681 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
     1741 gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat
     1801 cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag
     1861 tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc ggcgcgccca
     1921 gctgtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     1981 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct gaattcgcgg
     2041 ccgcttctag ttgacagcta gctcagtcct aggtattgtg ctagcccatg gcctcgacaa
     2101 taacctatta ggatcaggcc ctatgcgtcc ggcgacatac gaaccagaac agattattga
     2161 agcagggctg gccctacaag ctgaaggacg gaatatcacc gggttcgcac tacgtaacca
     2221 ggtgggtggc ggcaatccga cacgtctccg ccagatatgg gacgaatacc aggcttcaca
     2281 gagcacggtc gtcactgaac ccgttgccga gctgccagtg gaagtggctg aagaagtgaa
     2341 ggccgtctcc gccgcgctgt ccgaacgcat cacccagctg gcgacagaac tgaatgacaa
     2401 ggcggtccgg gctgctgaac gccgggttgc ggaagtcacg cgtgctgccg gtgaacagac
     2461 cgcacaggca gagcgggagc tggccgacgc cgcgcagaca gtcgacgacc tggaagaaaa
     2521 actggatgaa cttcaggaca gatatgacag tttgacgctg gcgctggagt cagaacgttc
     2581 actgcgtcag cagcatgatg tggagatggc ccagctgaaa gagcgtcttg cagccgctga
     2641 agagaatacc cgtcagcgag aggaacggta tcaggagcag aagacagtgc tacaggatgc
     2701 gcttaatgcg gagcaggcac agcacaaaaa cacgcgggaa gacctacaga aacgactgga
     2761 gcaaatttct gccgaagcta atgcgcgtac agaagaactg aagtctgaac gcgataaagt
     2821 caatactctc cttacccgcc ttgaatcgca ggaaaatgcg ctggcctcag aacgtcagca
     2881 gcatctggcc acccgcgaaa cgcttcagca acgcctcgag caggccatcg ctgacacgca
     2941 ggcgcgcgcc ggtgagattg cacttgaacg tgacagagtc agcagcctca ccgcaaggct
     3001 ggaatcgcag gaaaaggcct cctcggagca actggtgcgt atgggcagtg aaatagccag
     3061 tctgacagag cgttgcacac agctggaaaa ccagcgtgat gatgcccgtc tggagacgat
     3121 gggggagaaa gaaacggtcg cggcactgcg tggtgaggct gaagccctga agcgtcagaa
     3181 ccagtcactg atggcggcgc tttcaggcaa taaacagacc ggtggccaga atgcgtgata
     3241 ctagtagcgg ccgctgcag
//
