LOCUS       Exported                2268 bp ds-DNA     circular SYN 17-OCT-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2017 from SnapGene 4.0.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..198
                     /label=Pnis
                     /note="/ApEinfo_fwdcolor=#9eafd2
                     /ApEinfo_revcolor=#9eafd2"
     misc_feature    215..219
                     /label=BioBrick suffix
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     stem_loop       220..291
                     /label=E. coli his operon terminator
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     stem_loop       228..262
                     /label=Stem loop
                     /note="/ApEinfo_fwdcolor=#75c6a9
                     /ApEinfo_revcolor=#75c6a9"
     misc_feature    complement(355..374)
                     /label=Verification reverse (VR) primer binding site
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    complement(459..1073)
                     /label=rep (pMB1)
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     misc_feature    474
                     /label=ORI
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     stem_loop       1240..1345
                     /label=T0 terminator
                     /note="/ApEinfo_fwdcolor=#d59687
                     /ApEinfo_revcolor=#d59687"
     misc_feature    complement(1358..2017)
                     /label=Chloramphenicol resistance
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    2131..2150
                     /label=Verification forward (VF2) primer binding site
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     stem_loop       complement(2207..2210)
                     /label=end of terminator
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     stem_loop       complement(2220..2239)
                     /label=Stem loop
                     /note="/ApEinfo_fwdcolor=#b1ff67
                     /ApEinfo_revcolor=#b1ff67"
ORIGIN
        1 ctagtcttat aactatactg acaatagaaa cattaacaaa tctaaaacag tcttaattct
       61 atcttgagaa agtattggta ataatattat tgtcgataac gcgagcataa taaacggctc
      121 tgattaaatt ctgaagtttg ttagatacaa tgatttcgtt cgaaggaact acaaaataaa
      181 ttataaggag gcactcacta ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt
      241 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
      301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
      481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1201 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
     1261 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     1321 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     1381 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     1441 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     1501 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     1561 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     1621 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     1681 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     1741 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     1801 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     1861 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     1921 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     1981 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     2041 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     2101 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc
     2161 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
     2221 ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagag
//
