Difference between revisions of "Team:Arizona State/Design"

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<h2>Receiver Design</h2>
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Revision as of 19:41, 1 September 2017

Design for New Receivers

During the first steps of the design process, our iGEM team discussed what engineering aims our project wanted to achieve. It was an important first step in determining the goals for our research. We based our engineering aims on Endy's "Foundations for engineering biology," from 2005.

Engineering Aims for ASU iGEM 2017

  • Extending the natural function spectrum and expression of new genes
  • In vitro evolution- biological regulation on a genetic and biochemical level
  • Genetic circuit construction for Quorum Sensing
  • Creating a quorum sensing system and chassis

After determining our engineering goals and aims for this year's competition. Our team needed to do background research into quorum sensing to familiarize ourselves with the terminology going to be used for this competition. In addition to this, our team needed to reaffirm the 2016 ASU iGEM team's data collected from last year before going any further with designing our project. While confirming last year's team data, we discovered a major flaw in their design process of the receivers. These receivers were showed poor expression and only a select few were good enough to characterize. In addition to this, the system these receivers were cloned into did not show a good cloning ratio. In fact, it was very difficult to clone these receivers. After this, we realized we need to re-evaluate these receivers to determine whether it was the system we were cloning them in was bad, or the receivers themselves that needed to be redesigned.

From this, it was determined that the receivers themselves were bad and needed to be redesigned this year. From this realization, our design process was re-evaluated and is represented in the flow chart below:

Receiver Design Flowchart

Design Flowchart



Receiver Design Flowchart for Cloning

Design Flowchart 2

Receiver Design

Design Flowchart 2




What should this page contain?

  • Explanation of the engineering principles your team used in your design
  • Discussion of the design iterations your team went through
  • Experimental plan to test your designs
  • Endy, Drew. "Foundations for engineering biology." Nature 438.7067 (2005): 449

Inspiration

References

[1] Endy, Drew. "Foundations for engineering biology." Nature 438.7067 (2005): 449