Parts Used:

(this is what the iGEM website said to include ) ****This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!

AnthocyaninnConstruct

3gt

The gene 3gt is from the anthocyanin snthesis pathway and converts the initial molecule Pelargonidin into Anthocyanin. This gene is from the organism i dont actualy know. We have added this part to the registry as one of our new basic part submissions.

f3h

We will be useing the gene f3h as the first gene in our anthocyanin synthesis pathway, it comes from the organism Petroselinum crispum. It was added to the registry by the 2014 Darmstadt iGEM team, part BBa_K1497009. This gene will code for a protein that converts the initial molecule flavanone into dihydroflavonol.

dfr

The gene dfr is the second one in our anthocyanin synthesis pathway. We are using the biobrick part BBa_K1497010. It was added to the registry by the 2014 Darmstadt igem team. Their composite part BBa_K1497023 did not contain an promotors, ours will contain a T7 promotor.

ans

Melanin Construct

T7 Promoter

The Promoter we used for our Melanin Construct is T7 promoter, This allows us to control the production of Melanin. This promoter is from the T7 bacteriophage, it is a Virus that inserts its DNA into Bacteria in order to reproduce and stay alive. This promoter works with the T7 system inside the Escherichia coli strain BL21(DE3), (Fig 1.)


(Fig 1.) This image shows the T7 Promoter system, 1.Lactose inducible promoter 2.E. coli Ribosomal Binding site. 3.The Gene for T7 RNA polymerase. 4.Terminator 5.T7 RNA polymerase. 6.T7 promoter. 7.E. coli Ribosomal Binding site. 8.The gene/genes in our construct. 9.Terminator. 10.mRNA produced by the transcription of the construct.

This shows the process that occurs with the T7 System. We induce the Lactose inducible promoter with IPTG as it mimics the lactose and cannot be broken down by the cell. This allows it to continually perform transcription on the construct embedded in the Genome of the BL21(DE3) and thusly constantly produce the T7 RNA polymerase.

Ribosomal Binding Site

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