Difference between revisions of "Team:TECHNION-ISRAEL"

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<img class = "img-logo" src="https://static.igem.org/mediawiki/2017/2/2b/T--TECHNION-ISRAEL--tolback.png" alt="logo" style="width:740px; height: 380px; position:">
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<h3> A preventative treatment for autoimmune disease and allergies  
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<h1> Description: </h1>
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<b>Autoimmune Disease and Allergies: </b> <br>
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Autoimmune diseases and allergies are an increasingly common and often devastating phenomenon in the Western world. It is estimated that over 23 million people suffer from autoimmune diseases, and more than 50 million people suffer from allergies in the United States alone  .<br>
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Many treatments for both autoimmune disease  and allergies  have been attempted with varying degrees of success, but a single treatment modality for immune disorders has yet to be implemented. To date, there are still many autoimmune diseases lacking treatment.<br>
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<b> Our Solution: </b> <br>
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<h1> Abstract </h1>
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We intend to design a preventative treatment for allergies and autoimmune disease by utilizing the natural system of Central Tolerance. Central Tolerance is one of the primary mechanisms through which the immune system becomes tolerant to “self”. In this mechanism, exposure of developing lymphocytes to autoantigen leads to their deletion, thus precluding the possibility of an immune response to self-antigen.  Through genetic engineering and subsequent transfusion of hematopoietic stem cells we hope to induce widespread and continuous immune tolerance towards specific antigens that are known causes of allergic and autoimmune disease. To this end, we are designing a plasmid based platform that will allow inducible expression and display of target antigens on the membrane of hematopoietic stem cells, and their differentiated lineages. While the specific antigens we are working with represent only a fraction of the existing diseases and allergies, they serve as proof of concept for a modular and efficacious system capable of preventing many more autoimmune and allergic diseases.<br>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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<a href="https://2017.igem.org/Special:Upload">
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UPLOAD FILES
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<p> Autoimmune disease and allergies are an increasingly common phenomenon in the Western world. An estimated 73 million people suffer from allergies  and autoimmune disease  in the United States alone. To date, many of these diseases lack treatment and pose an incredible financial burden on both the patients and society.
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<p> We set out to create a preventative treatment for allergies and autoimmune disease by utilizing the innate mechanism of Central Tolerance. We’ve designed a modular plasmid based platform that will allow for inducible expression, and display, of target antigens on the membrane of Hematopoietic Stem Cells (HSCs). In our project, murine Hematopoietic Progenitor Cells (HPC-7) were used as a model for HSCs and transfected with our plasmid. Additionally, we attempted to show apoptosis in an immature B cell model (WEHI-231) as a result of our treatment, closely approximating the process of Central Tolerance. In the future this technology may be used to engineer HSCs harvested from cord blood and allow for a cost effective preventative treatment.
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{{:Team:TECHNION-ISRAEL/ex_spon}}

Revision as of 18:38, 13 September 2017



A preventative treatment for autoimmune disease and allergies



Abstract



Autoimmune disease and allergies are an increasingly common phenomenon in the Western world. An estimated 73 million people suffer from allergies and autoimmune disease in the United States alone. To date, many of these diseases lack treatment and pose an incredible financial burden on both the patients and society.

We set out to create a preventative treatment for allergies and autoimmune disease by utilizing the innate mechanism of Central Tolerance. We’ve designed a modular plasmid based platform that will allow for inducible expression, and display, of target antigens on the membrane of Hematopoietic Stem Cells (HSCs). In our project, murine Hematopoietic Progenitor Cells (HPC-7) were used as a model for HSCs and transfected with our plasmid. Additionally, we attempted to show apoptosis in an immature B cell model (WEHI-231) as a result of our treatment, closely approximating the process of Central Tolerance. In the future this technology may be used to engineer HSCs harvested from cord blood and allow for a cost effective preventative treatment.