Difference between revisions of "Team:Munich"

Line 6: Line 6:
 
<html>
 
<html>
 
<head>
 
<head>
 +
<link rel="stylesheet" type="text/css"
 +
href="https://2017.igem.org/Template:Munich/Header?action=raw&ctype=text/css" />
 
<style>
 
<style>
#home_logo, #sideMenu { display:none; }
 
    #sideMenu, #top_title, .patrollink  {display:none;}
 
    #content { width:100%; padding:0px;  margin-top:-7px; margin-left:0px;}
 
    body {background-color:white; font-size: 14px; }
 
    #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 
 
#HQ_page #myFooter td{
 
vertical-align: text-top;
 
}
 
 
#HQ_page td not(#myContent){
 
padding: 0;
 
}
 
 
#HQ_page table{
 
font-size: 14px;
 
}
 
 
hr{
 
height: 1px !important;
 
}
 
#HQ_page td{
 
border-style: none ;
 
vertical-align: middle;
 
}
 
 
#HQ_page table{
 
border-style: none !important;
 
margin: 0 !important;
 
}
 
 
html, body
 
{
 
margin: 0;
 
padding: 0;
 
font-family: "Helvetica", Arial, Serif;
 
}
 
.dropdown {
 
    width: 137px;
 
    margin: auto;
 
    position: relative;
 
    display: inline-block;
 
}
 
 
 
 
.dropbtnCascAid {
 
    background-image: url("https://static.igem.org/mediawiki/2017/b/ba/T--Munich--wiki_image_menu_cascaid_low.gif");
 
    position: relative;
 
    padding: 0px;
 
    border: none;
 
    cursor: pointer;
 
    width: 137px;
 
    height: 32px;
 
}
 
.dropdown:hover .dropbtnCascAid {
 
    background-image: url("https://static.igem.org/mediawiki/2017/1/1f/T--Munich--wiki_image_menu_cascaid_high.gif");
 
}
 
 
 
 
.dropbtnProject {
 
    background-image: url("https://static.igem.org/mediawiki/2017/b/ba/T--Munich--wiki_image_menu_project_low.gif");
 
    position: relative;
 
    padding: 0px;
 
    border: none;
 
    cursor: pointer;
 
    width: 137px;
 
    height: 32px;
 
}
 
.dropdown:hover .dropbtnProject {
 
    background-image: url("https://static.igem.org/mediawiki/2017/5/5d/T--Munich--wiki_image_menu_project_high.gif");
 
}
 
 
 
 
.dropbtnWetlab {
 
    background-image: url("https://static.igem.org/mediawiki/2017/9/9d/T--Munich--wiki_image_menu_wetlab_low.gif");
 
    position: relative;
 
    padding: 0px;
 
    border: none;
 
    cursor: pointer;
 
    width: 137px;
 
    height: 32px;
 
}
 
.dropdown:hover .dropbtnWetlab {
 
    background-image: url("https://static.igem.org/mediawiki/2017/e/e1/T--Munich--wiki_image_menu_wetlab_high.gif");
 
}
 
 
 
 
.dropbtnSoftHardware {
 
    background-image: url("https://static.igem.org/mediawiki/2017/b/bb/T--Munich--wiki_image_menu_softhardware_low.gif");
 
    position: relative;
 
    padding: 0px;
 
    border: none;
 
    cursor: pointer;
 
    width: 137px;
 
    height: 32px;
 
}
 
.dropdown:hover .dropbtnSoftHardware {
 
    background-image: url("https://static.igem.org/mediawiki/2017/1/18/T--Munich--wiki_image_menu_softhardware_high.gif");
 
}
 
 
 
 
.dropbtnModel {
 
    background-image: url("https://static.igem.org/mediawiki/2017/7/7b/T--Munich--wiki_image_menu_model_low.gif");
 
    position: relative;
 
    padding: 0px;
 
    border: none;
 
    cursor: pointer;
 
    width: 137px;
 
    height: 32px;
 
}
 
.dropdown:hover .dropbtnModel {
 
    background-image: url("https://static.igem.org/mediawiki/2017/3/3d/T--Munich--wiki_image_menu_model_high.gif");
 
}
 
 
 
 
.dropbtnHP {
 
    background-image: url("https://static.igem.org/mediawiki/2017/7/78/T--Munich--wiki_image_menu_hp_low.gif");
 
    position: relative;
 
    padding: 0px;
 
    border: none;
 
    cursor: pointer;
 
    width: 137px;
 
    height: 32px;
 
}
 
.dropdown:hover .dropbtnHP {
 
    background-image: url("https://static.igem.org/mediawiki/2017/d/df/T--Munich--wiki_image_menu_hp_high.gif");
 
}
 
 
 
 
.dropbtnTeam {
 
    background-image: url("https://static.igem.org/mediawiki/2017/f/fd/T--Munich--wiki_image_menu_team_low.gif");
 
    position: relative;
 
    padding: 0px;
 
    border: none;
 
    cursor: pointer;
 
    width: 137px;
 
    height: 32px;
 
}
 
.dropdown:hover .dropbtnTeam {
 
    background-image: url("https://static.igem.org/mediawiki/2017/5/57/T--Munich--wiki_image_menu_team_high.gif");
 
}
 
 
 
 
.dropdown-contentDescription {
 
    background-image: url("wiki_image_menu_description_low.gif");
 
    display: none;
 
    position: absolute;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentDescription {
 
    background-image: url("wiki_image_menu_description_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentDescription a {
 
    background-image: url("wiki_image_menu_description_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentDescription a:hover {
 
    background-image: url("wiki_image_menu_description_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentDesign {
 
    background-image: url("wiki_image_menu_design_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 64;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentDesign {
 
    background-image: url("wiki_image_menu_design_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentDesign a {
 
    background-image: url("wiki_image_menu_design_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentDesign a:hover {
 
    background-image: url("wiki_image_menu_design_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentProofofConcept {
 
    background-image: url("wiki_image_menu_proofofconcept_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 96;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentProofofConcept {
 
    background-image: url("wiki_image_menu_proofofconcept_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentProofofConcept a {
 
    background-image: url("wiki_image_menu_proofofconcept_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentProofofConcept a:hover {
 
    background-image: url("wiki_image_menu_proofofconcept_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentDemonstration {
 
    background-image: url("wiki_image_menu_demonstration_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 128;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentDemonstration {
 
    background-image: url("wiki_image_menu_demonstration_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentDemonstration a {
 
    background-image: url("wiki_image_menu_demonstration_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentDemonstration a:hover {
 
    background-image: url("wiki_image_menu_demonstration_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentApplication {
 
    background-image: url("wiki_image_menu_application_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 160;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentApplication {
 
    background-image: url("wiki_image_menu_application_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentApplication a {
 
    background-image: url("wiki_image_menu_application_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentApplication a:hover {
 
    background-image: url("wiki_image_menu_application_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentFinalResults {
 
    background-image: url("wiki_image_menu_finalresults_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 192;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentFinalResults {
 
    background-image: url("wiki_image_menu_finalresults_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentFinalResults a {
 
    background-image: url("wiki_image_menu_finalresults_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentFinalResults a:hover {
 
    background-image: url("wiki_image_menu_finalresults_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentEntrepreneurship {
 
    background-image: url("wiki_image_menu_entrepreneurship_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 224;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentEntrepreneurship {
 
    background-image: url("wiki_image_menu_entrepreneurship_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentEntrepreneurship a {
 
    background-image: url("wiki_image_menu_entrepreneurship_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentEntrepreneurship a:hover {
 
    background-image: url("wiki_image_menu_entrepreneurship_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentAchievements {
 
    background-image: url("wiki_image_menu_achievements_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 256;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentAchievements {
 
    background-image: url("wiki_image_menu_achievements_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentAchievements a {
 
    background-image: url("wiki_image_menu_achievements_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentAchievements a:hover {
 
    background-image: url("wiki_image_menu_achievements_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentProtocolsMethods {
 
    background-image: url("wiki_image_menu_protocolsmethods_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 32;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentProtocolsMethods {
 
    background-image: url("wiki_image_menu_protocolsmethods_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentProtocolsMethods a {
 
    background-image: url("wiki_image_menu_protocolsmethods_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentProtocolsMethods a:hover {
 
    background-image: url("wiki_image_menu_protocolsmethods_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentMaterials {
 
    background-image: url("wiki_image_menu_materials_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 64;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentMaterials {
 
    background-image: url("wiki_image_menu_materials_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentMaterials a {
 
    background-image: url("wiki_image_menu_materials_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentMaterials a:hover {
 
    background-image: url("wiki_image_menu_materials_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentLabJournal {
 
    background-image: url("wiki_image_menu_labjournal_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 96;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentLabJournal {
 
    background-image: url("wiki_image_menu_labjournal_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentLabJournal a {
 
    background-image: url("wiki_image_menu_labjournal_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentLabJournal a:hover {
 
    background-image: url("wiki_image_menu_labjournal_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentSafety {
 
    background-image: url("wiki_image_menu_safety_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 128;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentSafety {
 
    background-image: url("wiki_image_menu_safety_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentSafety a {
 
    background-image: url("wiki_image_menu_safety_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentSafety a:hover {
 
    background-image: url("wiki_image_menu_safety_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentParts {
 
    background-image: url("wiki_image_menu_parts_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 160;
 
    height: 32px;
 
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}
 
.dropdown:hover .dropdown-contentParts {
 
    background-image: url("wiki_image_menu_parts_high.gif");
 
    height: 32px;
 
    width: 137px;
 
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}
 
.dropdown-contentParts a {
 
    background-image: url("wiki_image_menu_parts_low.gif");
 
    height: 32px;
 
    width: 137px;
 
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    text-decoration: none;
 
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}
 
.dropdown-contentParts a:hover {
 
    background-image: url("wiki_image_menu_parts_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentInterlab {
 
    background-image: url("wiki_image_menu_interlab_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 192;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentInterlab {
 
    background-image: url("wiki_image_menu_interlab_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentInterlab a {
 
    background-image: url("wiki_image_menu_interlab_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
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}
 
.dropdown-contentInterlab a:hover {
 
    background-image: url("wiki_image_menu_interlab_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentSoftware {
 
    background-image: url("wiki_image_menu_software_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 32;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentSoftware {
 
    background-image: url("wiki_image_menu_software_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentSoftware a {
 
    background-image: url("wiki_image_menu_software_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentSoftware a:hover {
 
    background-image: url("wiki_image_menu_software_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentHardware {
 
    background-image: url("wiki_image_menu_hardware_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 64;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentHardware {
 
    background-image: url("wiki_image_menu_hardware_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentHardware a {
 
    background-image: url("wiki_image_menu_hardware_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentHardware a:hover {
 
    background-image: url("wiki_image_menu_hardware_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentHPSilver {
 
    background-image: url("wiki_image_menu_hpsilver_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 32;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentHPSilver {
 
    background-image: url("wiki_image_menu_hpsilver_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentHPSilver a {
 
    background-image: url("wiki_image_menu_hpsilver_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentHPSilver a:hover {
 
    background-image: url("wiki_image_menu_hpsilver_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentIHPgold {
 
    background-image: url("wiki_image_menu_ihpgold_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 64;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentIHPgold {
 
    background-image: url("wiki_image_menu_ihpgold_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentIHPgold a {
 
    background-image: url("wiki_image_menu_ihpgold_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentIHPgold a:hover {
 
    background-image: url("wiki_image_menu_ihpgold_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentPublicEngagement {
 
    background-image: url("wiki_image_menu_publicengagement_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 96;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentPublicEngagement {
 
    background-image: url("wiki_image_menu_publicengagement_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentPublicEngagement a {
 
    background-image: url("wiki_image_menu_publicengagement_low.gif");
 
    height: 32px;
 
    width: 137px;
 
    padding: 0px 0px;
 
    text-decoration: none;
 
    display: block;
 
}
 
.dropdown-contentPublicEngagement a:hover {
 
    background-image: url("wiki_image_menu_publicengagement_high.gif");
 
    height: 32px;
 
    width: 137px;
 
}
 
 
 
 
.dropdown-contentCollaborations {
 
    background-image: url("wiki_image_menu_collaborations_low.gif");
 
    display: none;
 
    position: absolute;
 
    top: 128;
 
    height: 32px;
 
    width: 137px;
 
    z-index: 1;
 
}
 
.dropdown:hover .dropdown-contentCollaborations {
 
    background-image: url("wiki_image_menu_collaborations_high.gif");
 
    height: 32px;
 
    width: 137px;
 
    display: block;
 
}
 
.dropdown-contentCollaborations a {
 
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Revision as of 13:03, 14 September 2017


Abstract

The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S RNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.

Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.

Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.

| The iGem Munich 2017 Team | 2017.07.13 |