Difference between revisions of "Team:Lethbridge HS/Composite Part"
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These genes will all be used in the cell to produce Anthocyanin out of Eriodictyol. The <i>yad</i>H has been shown to increase yields even though it is not directly involved in the pathway. The <i>3gt</i> as well as <i>yad</i> H are two of our original basic part submissions. This Construct is our composite part submission BBa_anthocyanincompsitepart. | These genes will all be used in the cell to produce Anthocyanin out of Eriodictyol. The <i>yad</i>H has been shown to increase yields even though it is not directly involved in the pathway. The <i>3gt</i> as well as <i>yad</i> H are two of our original basic part submissions. This Construct is our composite part submission BBa_anthocyanincompsitepart. | ||
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| − | <div style="text-align:center;"><center><img height=" | + | <div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/1/15/T--Lethbridge_HS--AnthocyaninPathway.png"><center></div> |
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<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/2/24/T--Lethbridge_HS--AnthocyaninConstruct.png"></center></div> | <div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/2/24/T--Lethbridge_HS--AnthocyaninConstruct.png"></center></div> | ||
Revision as of 00:44, 1 October 2017
Anthocyanin Construct
This construct is being used to convert our initial molecule Eriodictyol into our final molecule Anthocyanin. The genes are:
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
f3h Part BBa_K1497009
dfr Part BBa_K1497010
ans Part BBa_K1497002
3gt Part BBa_waitingforsubmission
yadH Part BBa_alsowaiting
Terminator Part BBa_B0015.
These genes will all be used in the cell to produce Anthocyanin out of Eriodictyol. The yadH has been shown to increase yields even though it is not directly involved in the pathway. The 3gt as well as yad H are two of our original basic part submissions. This Construct is our composite part submission BBa_anthocyanincompsitepart.
Zeaxanthin Construct
This construct is made up of the following genes:
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
crtY Part BBa_I742154.
crtZ Part BBa_I742157.
Terminator Part BBa_B0015.
These parts all come together in our composite part BBa_Zeaxanthinconstruct. This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.
Indigoidine Construct
Our Indigoidine construct is made up of:
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indB Part BBa_waiting for actual part submission.
indC Part BBa_K1152013
Terminator Part BBa_B0015.
These two genes come together to convert our initial molecule Glutamine into Indigoidine. The indB has been shown to increase the yields of Indigoidine as well as it is one of our original basic part submissions. This construct is part BBa_idkyet
Melanin Construct
The parts used in our Melanin construct are as follows:
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
MelA Part BBA_K274001.
Terminator Part BBa_B0015.
This construct allows us to make Melanin out of L-Tyrosine. it is Part BBa_waitandsee
