Difference between revisions of "Team:Sheffield/Hardware"
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| − | <p align="center">Detection methods that we considered are listed in the table below: | + | <p align="center">Detection methods that we considered are listed in the table below:</p> |
| − | To read a brief description about each detection method, click on their title in the table:</p> | + | <p align="center">To read a brief description about each detection method, click on their title in the table:</p> |
Revision as of 17:30, 12 October 2017
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Introduction
Much of the current lab equipment commonly used is very expensive. Due to this, some labs that have a smaller budget are limited to the amount of equipment they are able to provide, and as the equipment is very expensive to replace, labs that do have many pieces of equipment rarely update this machinery resulting in outdated hardware and software.
Our team wanted to devise a solution for this problem, and thus we started investigated the different types of methods currently employed for the detection of bacterial growth. A simple experiment, which we believe everyone should be able to perform without the need of expensive lab equipment. This will then allow future iGEM team who perhaps do not have access to these technologies will be able to build and design from our open source manual and allow them to perform essential experiment for the development of their projects, without the need of a large sum of money.
We focused on detection methods that may be adjusted to make automated measurements within a warm room, as this was the most applicable for our desired applications so, for example, we could use this device for an overnight experiment, allows us to run many more experiments with the bacteria day to day.
Detection Methods
Detection methods that we considered are listed in the table below:
To read a brief description about each detection method, click on their title in the table:
| Impedance microbiology | Capacitance | Opacity | Light microscopy | pH reading | |
|---|---|---|---|---|---|
| Advantages | Can use simple electronic components. | Can use simple electronic components. | Well known principle, used frequently in current labs. | Allows for the imaging of cells. | Can provide very sensitive information regarding the growth of bacteria. |
| Disadvantages | For a well in a 96 well plate the change would be too small to read. (explained in detail below) | For a well in a 96 well plate the change would be too small to read. (explained in detail below) | More complex circuitry required. | Does not provide quantitative information about the bacterial growth and requires sensitive camera equipment to achieve realistic magnification. | pH sensors can be very expensive. |
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