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<ul list-style-type="circle"> | <ul list-style-type="circle"> | ||
+ | <li>Selected for plasmid by growth on ampicilin LB agar plates</li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/1/1f/T--Newcastle--ecoli_transformation_bb.pdf" width="5px">Transformation protocol</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/1/1f/T--Newcastle--ecoli_transformation_bb.pdf" width="5px">Transformation protocol</a></li> | ||
<li>Performed by Bradley Brown</li> | <li>Performed by Bradley Brown</li> | ||
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<div class="entry CFPS April"> | <div class="entry CFPS April"> | ||
<h4>CFPS:</h4> | <h4>CFPS:</h4> | ||
− | <li> | + | <li>Confirmed successful transformation of plasmid into cells by visualisation of red colonies on the LB agar AMP plate: |
+ | <div class="img" style="height:300px"><img src="https://static.igem.org/mediawiki/2017/5/58/T--Newcastle--BB_pSB1AT3_J04450.png" height="100%"></div> | ||
</div> | </div> | ||
</ul> | </ul> |
Revision as of 00:20, 29 May 2017
Our wiki is currently under construction, please bear with us while we make it functional
Lab Book
All experiments and lab work are fully documented here. Click on the buttons below to filter the logs.
Month:
Aspect of the project:
April:
Week Commencing: 03/04/17
03/04/17:
- Insert work here
- Insert work here
- Insert work here
Synthetic Promoters:
Liquid Handling Robots Assembly Automation
General (CBCB)
04/04/17:
05/04/17:
- Transformed competent DH5α E. coli cells with BBa_J04450 in a pSB1AT3 plasmid backbone which will be used for the initial testing of E coli BL21-based CFPS systems
- Selected for plasmid by growth on ampicilin LB agar plates
- Transformation protocol
- Performed by Bradley Brown
CFPS:
06/04/17:
- Confirmed successful transformation of plasmid into cells by visualisation of red colonies on the LB agar AMP plate:
CFPS:
07/04/17:
Week Commencing: 10/04/17
10/04/17:
- Insert work here
Image