Difference between revisions of "Team:Newcastle/Results"

Our Experimental Results

Hover over elements of the diagram below to see what each part represents.
Click elements of the diagram below to see results for each section of our project.

Looking for Interlab Study related results? Click below!

Alternatively, click here to see a list of our experiments and results.
Want to learn more about our framework (above)? Head over to our description page!

Biochemcial Adaptor Modules: The Results

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Sarcosine Oxidase (Glyphosate to Formaldehyde)

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References

Detector Modules: The Results

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Synthetic Promoter Library

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References

Arsenic Biosensor

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References

Psicose Biosensor (Paris-Every Collaboration)

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References

Processor Modules: The Results

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Fim Standby Switch

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References

Signal Tuners

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References

Reporter Modules: The Results

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deGFP

BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References

Chromoproteins

BioBricks used: BBa_K2205016 (New),BBa_K2205017 (New),BBa_K2205018 (New), BBa_K1033915 (Uppsala 2013), BBa_K1033925 (Uppsala 2013), BBa_K1033929 (Uppsala 2013)

Rationale and Aim

The Sensynova multicellular biosensor platform has been developed to overcome the limitations identified by our team [hyperlink to human practices] that hamper the success in biosensors development. One of these limits regards the lack of modularity and reusability of the various components. Our platform design, based on the expression of three main modules (Detector, Processor and Output) by three E.coli strains in co-culture, allows the switch of possible variances for each module and the production of multiple customised biosensors.

[Insert image of modules here]

This section of the project is based on testing the modularity of the system by replacing the sfGFP output part of the Sensynova platform design with three different output chromoprotein variants; BBa_K1033929 (aeBlue), BBa_K1033925 (spisPink) and BBa_K1033915 (amajLime).

Background Information

All three selected chromoproteins were made and submitted to the iGEM registry by the Uppsala 2013 team.

They were chosen as variants to the sfGFP present in the Sensynova platform as they exhibit of strong colour readily observed in both LB cultures and in agar plates when expressed.

All three proteins have significant sequence homologies with proteins in the GFP family.

BBa_K1033915 – amajLime

The amajLime protein is a yellow-green chromoprotein extracted from the coral Anemonia majano. It was first extracted and characterized by Matz et al. under the name amFP486 (UniProtKB/Swiss-Prot: Q9U6Y6.1 GI: 56749103 GenBank: AF168421.1) and codon optimized for E coli by Genscript. The protein has an absorption maximum at 458 nm giving it a yellow-green colour visible to the naked eye.

BBa_K1033925 – spisPink

The spisPink protein is a pink chromoprotein extracted from the coral Stylophora pistillata. It was first extracted and characterized by Alieva et al. under the name spisCP (GenBank: ABB17971.1) and codon optimized for E coli by Genscript. The protein has an absorption maximum at 560 nm giving it a pink colour visible to the naked eye. The strong colour is readily observed in both LB or on agar plates after less than 24 hours of incubation.

BBa_K1033929 – aeBlue

The aeBlue protein is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equine. It was first extracted and characterized by Shkrob et al. 2005 under the name aeCP597 and codon optimised for E coli by Bioneer Corp. The protein has an absorption maximum at 597nm and a deep blue colour visible to the naked eye. The protein aeBlue has significant sequence homologies with proteins in the GFP family. The coding sequence for this protein was originally submitted to the registry as BBa_K1033916 by the 2012 Uppsala iGEM team.

Design Stage

In order to implement these three chromoprotein variants into the Sensynova platform, designs were made by replacing the sfGFP in the original reporter module with the parts detailed above that were ordered from the iGEM parts registry.

Implementation
Characterisation
Conclusions and Future Work
References

Sensynova Framework Testing (IPTG Sensor): The Results

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BioBricks used: BBa_0123456 (New), BBa_7890123 (Team_Name 20XX)

Rationale and Aim
Background Information
Design Stage
Implementation
Characterisation
Conclusions and Future Work
References