Difference between revisions of "Team:Newcastle/Notebook/LabBook"
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<div class="entry CFPS April"> | <div class="entry CFPS April"> | ||
<h4>CFPS:</h4> | <h4>CFPS:</h4> | ||
| − | <li>Transformed competent DH5α <i>E. coli</i> cells with <a href="http://parts.igem.org/Part:BBa_J04450">BBa_J04450</a> in a <a href="http://parts.igem.org/Part:pSB1AT3">pSB1AT3</a> plasmid backbone which will be used for the initial testing of <i>E coli</i> BL21-based CFPS systems | + | <li>Transformed competent DH5α <i>E. coli</i> cells with <a href="http://parts.igem.org/Part:BBa_J04450">BBa_J04450</a> in a <a href="http://parts.igem.org/Part:pSB1AT3">pSB1AT3</a> plasmid backbone which will be used for the initial testing of <i>E. coli</i> BL21-based CFPS systems |
<div class="img" style="height:300px"><a href="http://beta.labgeni.us/registries/parts_registry/?part=BBa_J04450"><img src="https://static.igem.org/mediawiki/2017/5/58/T--Newcastle--BB_pSB1AT3_J04450.png" height="100%"></a></div> | <div class="img" style="height:300px"><a href="http://beta.labgeni.us/registries/parts_registry/?part=BBa_J04450"><img src="https://static.igem.org/mediawiki/2017/5/58/T--Newcastle--BB_pSB1AT3_J04450.png" height="100%"></a></div> | ||
Revision as of 15:47, 29 May 2017
Our wiki is currently under construction, please bear with us while we make it functional
Lab Book
All experiments and lab work are fully documented here. Click on the buttons below to filter the entries.
Month:
Aspect of the project:
April:
Week Commencing: 03/04/17
03/04/17:
- Insert work here
- Insert work here
- Insert work here
Synthetic Promoters:
Liquid Handling Robots Assembly Automation
General
04/04/17:
05/04/17:
- Transformed competent DH5α E. coli cells with BBa_J04450 in a pSB1AT3 plasmid backbone which will be used for the initial testing of E. coli BL21-based CFPS systems
- Selected for plasmid by growth on ampicilin LB agar plates
- Transformation protocol
- Performed by Bradley Brown
CFPS:
06/04/17:
- Confirmed successful transformation of plasmid into cells by visualisation of red colonies on the LB agar AMP plate:
CFPS:
07/04/17:
Week Commencing: 10/04/17
10/04/17:
- Four Overnight cultures of J04450 in pSB1AT3 from transformation plates from 05/06/17
- 5 mL LB broth with 100 μg/mL ampicilin, incubated overnight at 37oC and 250 RPM
CFPS:
11/04/17:
- Used 1 mL of overnight culture from 10/04/17 to prepare glycerol stock of DH5α with pSB1AT3-J04450 plasmid
- Used remaining overnight cultures for miniprep to extract pSB1AT3-J04450 plasmid DNA
CFPS:
12/04/17:
- Measured concentration of minipreped pSB1AT3-J04450 plasmid DNA using a nanodrop
- MP1: 153.2 μg/mL
- MP2: 172.1 μg/mL
- MP3: 165.9 μg/mL
- MP4: 172.1 μg/mL
CFPS:
13/04/17:
14/04/17:
Week Commencing: 17/04/17
17/04/17:
18/04/17:
19/04/17:
20/04/17:
21/04/17:
Week Commencing: 17/04/17
24/04/17:
25/04/17:
26/04/17:
27/04/17:
28/04/17:
May:
Week Commencing: 01/05/17
01/05/17:
02/05/17:
03/05/17:
04/05/17:
05/05/17:
