Difference between revisions of "Team:CMUQ/InterLab"
Yasmin1304 (Talk | contribs) |
Yasmin1304 (Talk | contribs) |
||
| Line 482: | Line 482: | ||
<li> Resuspend gBLOCK in 20µL nuclease free dH2O</li> | <li> Resuspend gBLOCK in 20µL nuclease free dH2O</li> | ||
<li> Resuspend primers to make 100µM stock then dilute to make a 10µM stock</li> | <li> Resuspend primers to make 100µM stock then dilute to make a 10µM stock</li> | ||
| − | + | </ol> | |
<li>Add the following reagents to a PCR tube: </li> | <li>Add the following reagents to a PCR tube: </li> | ||
<li>Run the PCR machine </li> | <li>Run the PCR machine </li> | ||
Revision as of 21:21, 23 October 2017
BioSensor Lab
A1: T7 salt sensor
Introduction
The goal is amplify T7 salt sensor, digest it along with pSB1C3 plasmid with EcoRI and PstI
Materials
- Polymerase: Fusion Taq or Ampli tas (gold)
- Primers: PrefixF and SuffixR
- Nuclease free H2O
- 5xHF PCR Buffer
- gBlock DNA
- dNTPs
- PCR tubes
Procedure
// Initialize affix and add an offset to add affix class on scroll $('#mainNav').affix({ offset: { top: 100 } })
