Difference between revisions of "Team:CMUQ/protocols"

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<p style= "font-size: 20px;> Preparation of reagents </p>
 
<p style= "font-size: 20px;> Preparation of reagents </p>
  
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<li> Resuspend gBLOCK in 20µL nuclease free dH2O</li>
 
<li> Resuspend gBLOCK in 20µL nuclease free dH2O</li>
 
<li> Resuspend primers to make 100µM stock then dilute to make a 10µM stock</li>
 
<li> Resuspend primers to make 100µM stock then dilute to make a 10µM stock</li>
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<li>Run on an agarose gel, cut out the bands and purify.  </li>
 
<li>Run on an agarose gel, cut out the bands and purify.  </li>
 
<li> Send clones for sequencing  </li>
 
<li> Send clones for sequencing  </li>
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Revision as of 21:32, 23 October 2017

BioSensor Lab

A1: T7 salt sensor

Introduction

The goal is to amplify T7 salt sensor, digest it along with pSB1C3 plasmid with EcoRI and PstI

Materials

  • Polymerase: Fusion Taq or Ampli tas (gold)
  • Primers: PrefixF and SuffixR
  • Nuclease free H2O
  • 5xHF PCR Buffer
  • gBlock DNA
  • dNTPs
  • PCR tubes

Procedure

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