Difference between revisions of "Team:TokyoTech/Experiment/AHK4 Assay"
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<p style="font-family: Poppins;font-size: 16px"><p style="text-indent:1em">To establish a co-culture system, it is important that <i>E. coli</i> can receive and respond to signals produced by human cells. In our project, we decided to use iP, a cytokinin, as the signals and AHK4,a receptor of cytokinins, as the receptor. AHK4 can respond to iP by using a histidine-to-aspartate phosphorelay system existing in <i>E. coli</i>.</p> | <p style="font-family: Poppins;font-size: 16px"><p style="text-indent:1em">To establish a co-culture system, it is important that <i>E. coli</i> can receive and respond to signals produced by human cells. In our project, we decided to use iP, a cytokinin, as the signals and AHK4,a receptor of cytokinins, as the receptor. AHK4 can respond to iP by using a histidine-to-aspartate phosphorelay system existing in <i>E. coli</i>.</p> | ||
| − | <p style="text-indent:1em">A histidine-to-aspartate phosphorelay system is one of the most important signal transduction systems for prokaryotes to respond to environmental stimuli. This system includes two important compornents: a histidine kinase and a response regulator. The histidine kinase has sensor domains which enable to receive an environmental stimulus. After histidine kinase sense a stimulus, it | + | <p style="text-indent:1em">A histidine-to-aspartate phosphorelay system is one of the most important signal transduction systems for prokaryotes to respond to environmental stimuli. This system includes two important compornents: a histidine kinase and a response regulator. The histidine kinase has sensor domains which enable to receive an environmental stimulus. After histidine kinase sense a stimulus, it autophosphorelates and then the phosphate group is transferred to the response regulator, which in turn, promote expression of a certain gene corresponding to the stimulus.</p> |
<p style="text-indent:1em"> One of the His-to-Asp phosphorelay systems used in E. coli is composed of three components: RcsC, a histidine kinase, RcsD, a histidine-containing phosphotransmitter, RcsB, a response regulator. In this system, cps operon is activated through the pathway of RcsC→RcsD→RscB→cps. Previous studies showed that AHK4, a histidine kinase of Arabidopsis thaliana, can also take advantage of RcsD→RscB→cps pathway in E. coli by receiving cytokinins.</p> | <p style="text-indent:1em"> One of the His-to-Asp phosphorelay systems used in E. coli is composed of three components: RcsC, a histidine kinase, RcsD, a histidine-containing phosphotransmitter, RcsB, a response regulator. In this system, cps operon is activated through the pathway of RcsC→RcsD→RscB→cps. Previous studies showed that AHK4, a histidine kinase of Arabidopsis thaliana, can also take advantage of RcsD→RscB→cps pathway in E. coli by receiving cytokinins.</p> | ||
<p style="text-indent:1em">Since iP and AHK4 are only used in plants, we considered that employing this AHK4→RcsD→RscB→cps pathway enable us to establish communication between human cells and bacteria without activating any other unexpected genes.</p> | <p style="text-indent:1em">Since iP and AHK4 are only used in plants, we considered that employing this AHK4→RcsD→RscB→cps pathway enable us to establish communication between human cells and bacteria without activating any other unexpected genes.</p> | ||
Revision as of 01:54, 24 October 2017
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AHK4 Assay
Overview
To establish a co-culture system, it is important that E. coli can receive and respond to signals produced by human cells. In our project, we decided to use iP, a cytokinin, as the signals and AHK4,a receptor of cytokinins, as the receptor. AHK4 can respond to iP by using a histidine-to-aspartate phosphorelay system existing in E. coli.
A histidine-to-aspartate phosphorelay system is one of the most important signal transduction systems for prokaryotes to respond to environmental stimuli. This system includes two important compornents: a histidine kinase and a response regulator. The histidine kinase has sensor domains which enable to receive an environmental stimulus. After histidine kinase sense a stimulus, it autophosphorelates and then the phosphate group is transferred to the response regulator, which in turn, promote expression of a certain gene corresponding to the stimulus.
One of the His-to-Asp phosphorelay systems used in E. coli is composed of three components: RcsC, a histidine kinase, RcsD, a histidine-containing phosphotransmitter, RcsB, a response regulator. In this system, cps operon is activated through the pathway of RcsC→RcsD→RscB→cps. Previous studies showed that AHK4, a histidine kinase of Arabidopsis thaliana, can also take advantage of RcsD→RscB→cps pathway in E. coli by receiving cytokinins.
Since iP and AHK4 are only used in plants, we considered that employing this AHK4→RcsD→RscB→cps pathway enable us to establish communication between human cells and bacteria without activating any other unexpected genes.
Results
文章
文章
Discussion
考察
Reference
参考文献
Hajime Fujita: All Rights Reserved
