Helping TUST prepare for their first year of competition.

To begin with, we gave them construct suggestions, communicated with their team adviser. In 24/3/2017, we were invited to TUST to carried out recruiting propaganda for them, and provided our construction guide.

Helping NKU with their plasmid construction

What we did：

Helping NKU-iGEM construct their plasmid

The cleavage sites XbaI and SacI were added by PCR before and after lysase (lys)

Upper Primer：gcTCTAGAATGAAATACCTGCTGCCGAC

Lower Primer：cGAGCTCtcaatgcgtttccataatagcagc

After construction

After the PCR product was harvested then cleavage：

Cutsmart® buffer 5μl

XbaI 1μl

SacI 1μl

PCR product 43μl

After overnight digestion with the linearized plasmid pEX18 for 1 hr Transformation of the large intestine

PxylA-xylR was cleaved by SacI on plasmid pAX01

The pEX18-lys, which has been added with lys, was linearized by SacI single digestion

The two are connected after 1hr

Transform into E.coli

E.coli after transformation

E.coli after transformation

Helped us to determine the fluorescence intensity of pRS416-CUP1p-RFP without copper induction in Saccharomyces cerevisiae BY4742 and BY4741.

Cultures were incubated overnight in SC-URA medium

Take the bacteria to the new SC-URA to adjust the OD600 value to 0.1 for 24 hours

Fluorescence was measured

Excitation 472nm

Radiation 532nm

In the case of

the data provided to iGEM-Tianjin for reference

the data provided to iGEM-Tianjin for reference

Test the mini system for OUC

Mini system is a system composed of a mini promoter (about 100bp) and a mini terminator, and it is applicable for yeast. Because of the similarity of our biological chassis, they reached out to us and wanted us to run some control experiments for them. Using the same protocol and their mini system, we need to transform the system into our Saccharomyces cerevisiae in order to test if the plasmid will still function the same.

Protocol

2. Protocol: initial OD600 = 0.01 Test OD600 and threshold fluorescence every other 5-6 hours Exciting light λ=502/Emissive light λ=532

Result

This is our testing result for team OUC, turns out that our results reflects the same conclution. Thanks OUC for thiet trust and we are so glad that we helped.

Sent a Collaboration Request and construct an alliance to build a worldwide database.

We came up with the idea that we could gather all the iGEM team whose project is about water pollution treatment together and build an alliance to unite all our social impact, knowledge and geographical advantages.

2017.08.12

2. In 12th of August, we had a voice conferencing with SJTU/SCUT/XMU/UCAS/JLU/FAFU, we discussed about how we want to use this alliance and came up with 2 conclusion: 1. Build a worldwide database containing contents of heavy metals in soil 2. Mutual improve our social impact

2017.08.23

In 23th of August, we sent a Collaboration Request to iGEM official website, the next day Ana Sifuentes replied our message and posted our request in the iGEM official website,

and we received the response of many teams like team EXETER and team CSMU NCHU TAIWAN, with their information, we construct a database which contains the worldwide data of contents of Cu2+/Cd2+ in soil or water. And we build it based on map of the world. We received team CSMU NCHU TAIWAN’s kindly help, they offered us information about major metal pollution incidents in Taiwan as well as the real time monitoring data of places that have the potential to outbreak serious pollution incidents oneday.

Helping CQU construct their team and sign up for their first year

We offered team CQU China many constructing advice including finding team advisor and allocating funds. Next yeat will be their first year joining iGEM, and we will continue helping them until they are ready for their Gamboree. Hope their result in next year will be great!

Filmed a biosafety video together with other 12 teams

12 teams gather together to film a biosafety video, every team took different topics, but all based on Yale biosafety manual.

This one goes deeper

A good rule for rocket experimenters to follow is this: always assume that it will explode. The vehicle explodes, literally explodes, off the pad. The simulator shakes you a little bit, but the actual liftoff shakes your entire body and soul.

Even deeper

The path of the righteous man is beset on all sides by the iniquities of the selfish and the tyranny of evil men. Blessed is he who, in the name of charity and good will, shepherds the weak through the valley of darkness, for he is truly his brother's keeper and the finder of lost children.