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Nevertheless, we were afraid that the time under 37 degree Celsius or above is too short for the bacteria to produce enough amount of BFP. So we decide to use an irreversible inhibitor, and then we found the Rhl promoter. When the protein RhlI (K1541017)and RhlR (C0171) both exist, Prhl will continuously work without consuming the proteins, and thus will have enough time to produce BFP. Eventually, we designed this biobrick: Pcon RBS RhlI RBS RhlR Ter Ter + Prhl RBS BFP Ter Ter | Nevertheless, we were afraid that the time under 37 degree Celsius or above is too short for the bacteria to produce enough amount of BFP. So we decide to use an irreversible inhibitor, and then we found the Rhl promoter. When the protein RhlI (K1541017)and RhlR (C0171) both exist, Prhl will continuously work without consuming the proteins, and thus will have enough time to produce BFP. Eventually, we designed this biobrick: Pcon RBS RhlI RBS RhlR Ter Ter + Prhl RBS BFP Ter Ter | ||
Considering that our products might be damaged, causing the bacteria inside dead, we designed a mechanism to guarantee that our product is able to use. We found that if we put an LVA tag behind the chromoprotein, it will degrade much faster. So our concept is to make the bacteria produce the chromoprotein constantly, and it will be colorful when it is working. Nonetheless, when the bacteria weren’t alive anymore, the remain color will degrade fast and eventually become colorless. At the end, we designed this biobrick: Pcon RBS cj-Blue-lva Ter Ter (note that cj-Blue looks green) | Considering that our products might be damaged, causing the bacteria inside dead, we designed a mechanism to guarantee that our product is able to use. We found that if we put an LVA tag behind the chromoprotein, it will degrade much faster. So our concept is to make the bacteria produce the chromoprotein constantly, and it will be colorful when it is working. Nonetheless, when the bacteria weren’t alive anymore, the remain color will degrade fast and eventually become colorless. At the end, we designed this biobrick: Pcon RBS cj-Blue-lva Ter Ter (note that cj-Blue looks green) | ||
− | Lastly, since it would be difficult to transform more than three plasmid into the bacteria, we combined two of them with one of them in the reverse direction (we are afraid of that the gene behind will express poorly), and try to make the sequence as short as possible. So the final biobrick is | + | Lastly, since it would be difficult to transform more than three plasmid into the bacteria, we combined two of them with one of them in the reverse direction (we are afraid of that the gene behind will express poorly), and try to make the sequence as short as possible. So the final biobrick is show as follow.</div> |
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</section> | </section> | ||
</h1> | </h1> |
Revision as of 04:23, 26 October 2017