|
|
| Line 15: |
Line 15: |
| | | | |
| | <section style="display:table;background:none;padding:0px !important;z-index:100; "> | | <section style="display:table;background:none;padding:0px !important;z-index:100; "> |
| − | <h1 style="vertical-align:bottom;display:table-cell; width:40%;font-size:40pt;letter-spacing: 0.1em;z-index:120;text-align: center;">Entrepreneurship</h1> | + | <h1 style="vertical-align:bottom;display:table-cell; width:40%;font-size:40pt;letter-spacing: 0.1em;z-index:120;text-align: center;">Experiments Overview</h1> |
| − | <img style="vertical-align:bottom;display:table-cell; width:100%;" src="https://static.igem.org/mediawiki/2017/d/d0/T--INSA-UPS_France--Entrepreneurship_croco.png" alt=""> | + | <img style="vertical-align:bottom;display:table-cell; width:100%;" src="https://static.igem.org/mediawiki/2017/0/08/T--INSA-UPS_France--Experiments_croco.png" alt=""> |
| | </section> | | </section> |
| | | | |
| | <section> | | <section> |
| − | <h1>Commercialize a novel system for saving lives</h1>
| |
| | <p> | | <p> |
| − | With such an innovative system of synthetic communication between microorganisms dedicated to fight cholera, an ongoing human health issue, it would be a nonsense not to exploit it for saving lives. | + | This Realisations section describes all our wet lab work, based on our strategy. We divided this section into the <a href="">Clonings page</a> and the <a href="">Results page</a>: the first shows how we integrated the parts of our <a href="">Design</a> into our chassis, and the second shows the experiments we did to further validate their functionnality. Both are presented for each of our eight modules: |
| | </p> | | </p> |
| − | <p>
| + | </section> |
| − | In this part of the project, our team developed an approach to commercialize our system. To achieve this goal, we first collected <b>testimonies</b> on two topics:
| + | |
| − | </p>
| + | |
| − | <ul>
| + | |
| − | <li>a reflexion on the prototype: how can we best adapt and optimize our system to the current cholera epidemics?</li>
| + | |
| − | <li>an entrepreneurship reflexion: how to set up a company? What shall be our values?</li>
| + | |
| − | </ul>
| + | |
| − | <p>
| + | |
| − | We therefore met successful entrepreneurs in the field of biotechnology and business developer (Marc Lemonnier from the start-up Antabio, Pierre Monsan from Toulouse White Biotechnology, Pierre-Alain Hoffmann from the CRITT Bio-Industries), people that developed a business to treat water, especially from V. cholerae (Christophe Campéri-Ginestet from Sunwaterlife), humanitarians from NGOs that daily face cholera (Claire Salvador from Doctors Without Borders and Alama Keita from UNICEF) and finally, more generally to Westerners. These rich discussions have helped us to deeply understand the cholera context and to adapt our positioning in a viable way for us and sustainable for our targeted users.
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | Consequently, a <b>scope statement</b> has been established, detailing the functional and technical features of our product.
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | In parallel, an <a href="https://2017.igem.org/Team:INSA-UPS_France/HP/Gold_Integrated">ethical matrix</a> was built to highlight the important features to work on and to provide a decision tool so that our system is ethically acceptable.
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | Then, we had to conceive the device following as closely as possible the scope statement: How to best contain GMMs? How to make user-friendly a device? What materials can be used to combine quality and price? Once again, the testimonies and the ethical matrix helped us to reflect about that. Besides, diffusion tests were carried out by our team to choose the best materials of the device. After answering these questions, the device containing our system was modeled and printed in 3D thanks to Jean-Jacques Dumas help, a professional in the design of industrial products.
| + | |
| | | | |
| − | </p>
| + | <section style="background:none;"> |
| − | <p> | + | <img src="" alt=""> |
| − | Finally, once our product created, our team developed a <b>business plan</b> in order to project ourselves into a business creation project, define the action plan to be implemented to exploit this opportunity and how that will result in financial terms. Here, we were assisted by Pierre-Alain Hoffmann, Deputy Director of the business incubator named CRITT Bio-Industries, who has a lot of knowledge in business creation and development.
| + | </section> |
| | | | |
| − | </p>
| + | <section> |
| − | <figure> | + | <h1> Mimicking Vibrio sp. presence with an engineered E. coli</h1> |
| − | <img src="https://static.igem.org/mediawiki/2017/f/f2/T--INSA-UPS_France--Entrepreneurship_plan.png" style="max-width: 400px;" alt="">
| + | <p>We engineered here E. coli to mimic for safety reason the presence of Vibrio species.</p> |
| − | <figcaption>Entrepreneurship approach to commercialize our project Croc’n Cholera </figcaption>
| + | |
| − | </figure>
| + | |
| | </section> | | </section> |
| | | | |
| − | <style> | + | <section> |
| − | .overview_pe{ | + | <h1> E. coli producing C8-CAI-1 molecules can be sensed by V. harveyi</h1> |
| − | text-align: center;
| + | <p>In this module, we created synthetic communication between our engineered E. coli and V. harveyi.</p> |
| − | display:table;
| + | </section> |
| − | table-layout: fixed;
| + | |
| − | } | + | |
| | | | |
| − | .pe_category_ov{
| + | <section> |
| − | display: table-cell;
| + | <h1>Modification of V. harveyi to detect both C8-CAI-1 and CAI-1</h1> |
| − | height:100%;
| + | <p>Here, we present our first effective modification of V. harveyi and its perspectives for the project.</p> |
| − | vertical-align: top;
| + | </section> |
| − | width:25%;
| + | |
| − | }
| + | |
| − | .pe-category-inside{ | + | |
| − | background:rgba(255,255,255,0.2);
| + | |
| − | border-radius: 20px;
| + | |
| − | overflow: hidden;
| + | |
| − | margin:10px;
| + | |
| − | cursor:pointer;
| + | |
| − | border:solid 3px transparent;
| + | |
| − | }
| + | |
| − | .pe-category-inside img{ | + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | .pe-category-inside p{
| + | |
| − | padding:20px;
| + | |
| − | text-align: justify;
| + | |
| − | }
| + | |
| − | .pe-category-inside.category-selected{
| + | |
| − | border: solid 3px black;
| + | |
| − | }
| + | |
| | | | |
| − | .content-right{
| + | <section> |
| − | width:80%;
| + | <h1>Establishing production of diacetyl to establish communication between prokaryotic and eukaryotic cells</h1> |
| − | margin-left:20%;
| + | <p>This module is dedicated to the production of diacetyl by bacteria, as a signal relay toward yeast.</p> |
| − | }
| + | |
| − | .summary-left{
| + | |
| − | width:18%;
| + | |
| − | float:left;
| + | |
| − | }
| + | |
| − | .summary-left{
| + | |
| − | cursor:pointer;
| + | |
| − | }
| + | |
| − | .summary-left.summary-fixed{
| + | |
| − | position:fixed;
| + | |
| − | top:140px;
| + | |
| − | width:10%;
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | | + | |
| − | | + | |
| − | <div class="overview_pe">
| + | |
| − | <div class="pe_category_ov">
| + | |
| − | <div class="pe-category-inside">
| + | |
| − | <a href=""><h2>Testimonies</h2></a>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="pe_category_ov">
| + | |
| − | <div class="pe-category-inside category-selected" data-target="1">
| + | |
| − | <h2>Scope Statement</h2>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="pe_category_ov">
| + | |
| − | <div class="pe-category-inside">
| + | |
| − | <a href=""><h2>Device Conception</h2></a>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="pe_category_ov">
| + | |
| − | <div class="pe-category-inside" data-target="2">
| + | |
| − | <h2>Business Plan</h2>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <style>
| + | |
| − | .category-content.visible{
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − | .category-content{
| + | |
| − | display: none;
| + | |
| − | }
| + | |
| − | </style>
| + | |
| − | | + | |
| − | <div class="category-content visible" id="1">
| + | |
| − | <div class="summary-left">
| + | |
| − | <ul>
| + | |
| − | <li data-number="1" style="list-style-type: none;">Scope Statement</li>
| + | |
| − | <li data-number="1">1- Project presentation</li>
| + | |
| − | <li data-number="2">2- Project requirements</li>
| + | |
| − | <li data-number="3">Conclusion</li>
| + | |
| − | <li data-number="4">References</li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | <div class="content-right">
| + | |
| − | <section id="sc1"> | + | |
| − | <h1>Chapter 1 - Project presentation</h1> | + | |
| − | <h2>Context</h2>
| + | |
| − | <p>
| + | |
| − | Cholera is a diarrhea-causing-disease that has affected the entire world through many epidemics throughout history. Today it still strikes developing countries, war-torn countries or those affected by natural disasters. It is contracted after ingestion of water contaminated with the pathogenic bacterium <i>Vibrio cholerae</i>. The main cause of a cholera epidemic is the lack of drinking water and sanitation resources in affected countries. According to the WHO, between 1.3 and 4 million cases are reported worldwide each year and 21,000 to 143,000 deaths.
| + | |
| − | </p>
| + | |
| − | <p> | + | |
| − | Rehydratation is a very efficient treatment to cure the patients, however some people can not get this treatment early enough because they live in remote areas or can not have access to drinkable water. Thus a solution to treat water in these areas has to be found. Current preventive solutions (sterilizing filtration, water treatment by chlorination, etc.) are expensive or difficult to set up.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <h2>Purpose of the project</h2>
| + | |
| − | <p>
| + | |
| − | Our team wants to make a new system of detection and purification of water contaminated with the pathogenic bacterium <i>Vibrio cholerae</i>. This device will consist of two elements:
| + | |
| − | </p>
| + | |
| − | <ul>
| + | |
| − | <li>A sensor to detect the presence of <i>V. cholerae</i> in water consisting in engineered <i>Vibrio harveyi</i>.</li>
| + | |
| − | <li>
| + | |
| − | An effector to secrete a cocktail of antimicrobial peptides (AMPs) consisting in engineered <i>Pichia pastoris</i>. In Nature, these AMPs are successfully used by the crocodile immune system against bacteria. The peptides used in our system have a broad spectrum of action and are particularly effective against <i>V. cholerae</i>.</li>
| + | |
| − | </ul>
| + | |
| − | <p>
| + | |
| − | The device will be designed for local populations. Thus, the goal is to set up a system as suitable as possible to their users for the purification of water contaminated with cholera.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <h2>Organization of the project</h2>
| + | |
| − | <p>
| + | |
| − | This project is run by a group of nine students from different universities: Paul Sabatier University (Toulouse), INSA Lyon and INSA Toulouse. It is supervised by Stéphanie Heux and Brice Enjalbert, researchers at LISBP (Toulouse), assisted by eleven other researchers. The team was formed in January 2017 and will work on this project until November 2017, date of the final restitution at the MIT, Boston.
| + | |
| − | </p>
| + | |
| | </section> | | </section> |
| | | | |
| − | <section id="sc2"> | + | <section> |
| − | <h1>Chapter 2 - Project requirements</h1> | + | <h1> Diacetyl detection by Pichia pastoris</h1> |
| − | | + | <p>This presents are modification and first assays to engineered P. pastoris for diacetyl detection.</p> |
| − | <h2>Functional specifications</h2>
| + | |
| − | <h3>Detection of <i>Vibrio cholerae</i></h3>
| + | |
| − | | + | |
| − | <h4>Description</h4>
| + | |
| − | <p> | + | |
| − | Our system must <b>detect the presence of <i>Vibrio cholerae</i></b> in water so that the antimicrobial peptides (AMPs) are spread in flow only if it contains <i>Vibrio cholerae</i>. In fact, the production of AMPs has to be controlled for two reasons. On the one hand, if water is drinkable, there is no need for AMPs production. On the other hand, other microorganisms (either from the user microbiota or the nearby environment) won’t develop peptide resistance.
| + | |
| − | </p>
| + | |
| − | <h4>Constraints</h4>
| + | |
| − | <p>
| + | |
| − | To develop the disease, the minimal quantity of <b>V. cholerae</b> cells a human being must ingest is about 10<sup>4</sup> cells, in one dose of water<sup>1, 2</sup>. The constraints are the following:
| + | |
| − | </p>
| + | |
| − | <ul>
| + | |
| − | <li>to <b>detect this concentration</b> of cells </li>
| + | |
| − | <li>to <b>activate</b> AMP production for water purification <b>only starting from this concentration</b></li>
| + | |
| − | </ul>
| + | |
| − | <h4>Priority</h4>
| + | |
| − | <p>
| + | |
| − | Detection is not the top priority of our system. Purification could work continuously but the implementation of this function is better for safety reason. Moreover, according to the testimonies, the detection function of our system is a new and strong point in the treatment of water contaminated with <i>V. cholerae</i>.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <h3>Purification of water</h3>
| + | |
| − | <h4>Description</h4>
| + | |
| − | <p>
| + | |
| − | The other aim of our project is to <b>wipe <i>V. cholerae</i> out of water</b>. The system has to produce antimicrobial peptides when water is contaminated with <i>V. cholerae</i>, i.e. when water contains amounts of microorganism superior to the minimum toxic concentration (about 10<sup>4</sup> cells in one dose of water<sup>1</sup>). The AMPs used for the project have a broad spectrum of action against microorganisms, but they are particularly efficient against <i>V. cholerae</i> (Leucrocine I : MIC = 0.156 µg/mL<sup>3</sup> (>52µg/mL<sup>4</sup>); D-NY15: MIC = 27 µg/mL<sup>4</sup>; cOT2: MIC = 29.22 µg/mL<sup>5</sup>). The goal is to produce these peptides until <i>V. cholerae</i> is wiped out and water becomes drinkable or usable without being dangerous.
| + | |
| − | </p>
| + | |
| − | <h4>Constraints</h4>
| + | |
| − | <ul>
| + | |
| − | <li>The AMPs have to be correctly <b>synthesized</b> and <b>secreted</b>.</li>
| + | |
| − | <li>They must be <b>stable</b> enough to keep their activities in muddy water for example. </li>
| + | |
| − | <li>The AMPs must properly <b>diffuse</b> to target bacteria. </li>
| + | |
| − | <li>Several <b>factors</b> have to be taken into account: temperature in affected countries (around 40°C (104°F)), the viscosity of water, its pH, etc.</li>
| + | |
| − | </ul>
| + | |
| − | <h4>Priority</h4>
| + | |
| − | <p>
| + | |
| − | Purification of water contaminated with <i>V. cholerae</i> is the central part of our project: the priority is high. Without it, which is the first goal of the project, the contract won’t be fulfilled.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <h2>Technical specifications</h2>
| + | |
| − | <h3>Safety</h3>
| + | |
| − | <h4>Ingestion of the GMMs</h4>
| + | |
| − | <p>
| + | |
| − | The exchanges of molecules between the compartment containing GMMs and water must be feasible but the GMMs must not go into water. Actually, on the one hand, the CAI-1 molecule of the <i>V. cholerae</i> quorum sensing must diffuse to the compartment containing the detecting bacteria, <i>V. harveyi.</i> On the other hand, the AMPs produced by <i>P. pastoris</i> must go from the compartment containing the GMMs to the water contaminated with <i>V. cholerae</i>. However, class I <b>GMMs must be kept out of the purified water</b>.
| + | |
| − | </p>
| + | |
| − | <h4>Spreading of the GMMs</h4>
| + | |
| − | <p>
| + | |
| − | GMMs must <b>not be spread in the environment</b>. The compartment containing them must be impact- and break-resistant. People need to be aware that GMMs should not be disseminated in the environment.
| + | |
| − | </p>
| + | |
| − | <h4>Human toxicity of the AMPs</h4>
| + | |
| − | <p>
| + | |
| − | AMPs will be produced and released in water with the aim of wiping out <i>V. cholerae</i>. The amount of AMP remaining in water should <b>not be toxic to humans</b>. Experimentations must be done to know the quantity of AMPs in the water supposed to be drunk and especially their effects on epithelial cells (esophagus, stomach, intestines, etc.).
| + | |
| − | </p>
| + | |
| − | <h4>Limiting media leaks</h4>
| + | |
| − | <p>
| + | |
| − | The proportion of nutrients should be correctly calculated so that they are all consumed during the lifetime of GMMs. An excess of nutrients in the compartment could then diffuse into the water and cause the emergence of new microorganisms.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <h3>Environmental</h3>
| + | |
| − | <h4>Waste management of the GMMs</h4>
| + | |
| − | <p>
| + | |
| − | As GMMs are used in this system, it is essential to think about their waste management. After the use of the GMMs for the detection of <i>V. cholerae</i> and the purification of water, they must be killed before throwing them away. The questions are: <b>how to kill them and where to dump the GMMs?</b>
| + | |
| − | </p>
| + | |
| − | <h4>Recyclable plastic</h4>
| + | |
| − | <p>
| + | |
| − | In order to have an eco-friendly approach, the plastic used for the device must be recyclable.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <h3>Material</h3>
| + | |
| − | <h4>GMMs storage</h4>
| + | |
| − | <p>
| + | |
| − | GMMs (<i>V. harveyi</i> and <i>P. pastoris</i>) must be <b>confined in the same compartment</b> because they need to interact with each other. The microorganisms must be kept in this compartment. If they are freeze-dried, the water to be treated must be able to rehydrate the microorganisms. Therefore, water must be able to enter into the compartment.
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | To sum up, the compartment containing the microorganisms must hold them back but also, allow exchanges of CAI-1 molecules, AMPs and water between the two compartments of the device.
| + | |
| − | </p>
| + | |
| − | <h4>Easy to use</h4>
| + | |
| − | <p>
| + | |
| − | In developing countries, war-torn countries or those affected by natural disasters, people <b>cannot possess a complex device</b> from both a scientific and technical point of view.
| + | |
| − | </p>
| + | |
| − | <h4>Transportable</h4>
| + | |
| − | <p>
| + | |
| − | The device must be <b>easy to transport</b> in order to reach the populations of remote villages or in conditions of natural disaster and armed conflict.
| + | |
| − | </p>
| + | |
| − | <h4>Treatment speed</h4>
| + | |
| − | <p>
| + | |
| − | The treatment <b>speed</b> must be reasonable compared to the volume of treated water.
| + | |
| − | </p>
| + | |
| − | <h4>Water treatment capacity</h4>
| + | |
| − | <p>
| + | |
| − | The water treatment <b>capacity</b> must be adapted to the device function and to the capacity of the microorganisms to detect and treat the water. According to Alama Keita (UNICEF), our device must be able to purify around 11,025 L of water in a week for a village.
| + | |
| − | </p>
| + | |
| − | <h4>Strength</h4>
| + | |
| − | <p>
| + | |
| − | The device has to be built with robust materials (refer to paragraph “Spreading of the GMMs”).
| + | |
| − | </p>
| + | |
| − | <h4>Water taste</h4>
| + | |
| − | <p>
| + | |
| − | Water treatment <b>must not modified its taste</b> so that the product can be easily accepted.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <h3>Economical</h3>
| + | |
| − | <p>
| + | |
| − | The <b>price</b> must be adapted to similar options offered on the market.
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | Chloramine tablets are often used to treat water. In order to be competitive on the market, it is necessary to bring our price into line with our competitors. Therefore, to give us an order of magnitude, cost of chloramine tablets to treat the same volume of water as our product can be calculated.
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | The product “Chloramine 60 Tablets from SANOFI AVENTIS BELGIUM”<sup>6</sup> contains 60 tablets of chloramine and costs 3.69€. According to the instructions, 1 to 4 tablets must be dissolved in 25L of water, depending on the water pollution degree. One tablet costs 3.69/60=0.0615€. Thus, treating 25 L of water costs up to 246cts (4 tablets) and up to 9.84cts for 1L.
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | To conclude, treating water with our system should not cost more than 10cts per L.
| + | |
| − | </p>
| + | |
| | </section> | | </section> |
| | | | |
| − | <section id="sc3"> | + | <section> |
| − | <h1>Conclusion about the scope statement</h1> | + | <h1> P. pastoris is able to produce functional antimicrobial peptides</h1> |
| − | <p> | + | <p>Finally, this module described the efficiency of a yeast-produced crocodile AMP on V. harveyi.</p> |
| − | To conclude, the system must be able to detect <i>V. cholerae</i> and release AMPs from a threshold concentration of pathogenic bacterium in water. The water treatment aspect thanks to AMPs is essential.
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | The device that we have to create must gather technical criteria from an environmental, safety, material and economic point of view that we have listed in the scope statement. These specifications were defined taking into account the advice we received from the people we met throughout our project.
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | The design of the device will have to best fit these constraints to be <b>accepted by our potential future customers.</b>
| + | |
| − | </p>
| + | |
| | </section> | | </section> |
| | | | |
| − | <section id="sc4"> | + | <section> |
| − | <h1>References</h1> | + | <h1>Co-cultivate P. pastoris and V. harveyi is possible</h1> |
| − | <ol>
| + | <p>Toward a realistic solution, we tested here how to co-culture how consortium microoganisms.</p> |
| − | <li>
| + | |
| − | Baron, S. (Ed.). (1996). <i>Medical Microbiology</i> (4th ed.). Galveston (TX): University of Texas Medical Branch at Galveston.
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | Nelson et al. (2009). <i>Modeling multiple ecosystem services, biodiversity conservation, commodity production, and tradeoffs at landscape scales.</i> Front Ecol Environ, 7(1): 4-11.
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | Pata, S., Yaraksa, N., Daduang, S., Temsiripong, Y., Svasti, J., Araki, T., & Thammasirirak, S. (2011). <i>Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts. Developmental and Comparative Immunology, 35</i>(5), 545–553. https://doi.org/10.1016/j.dci.2010.12.011
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | Prajanban, B.-O., Jangpromma, N., Araki, T., & Klaynongsruang, S. (2017). <i>Antimicrobial effects of novel peptides cOT2 and sOT2 derived from Crocodylus siamensis and Pelodiscus sinensis ovotransferrins.</i> Biochimica Et Biophysica Acta<, <i>1859</i>(5), 860–869. https://doi.org/10.1016/j.bbamem.2017.01.035
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | Yaraksa, N., Anunthawan, T., Theansungnoen, T., Daduang, S., Araki, T., Dhiravisit, A., & Thammasirirak, S. (2014). <i>Design and synthesis of cationic antibacterial peptide based on Leucrocin I sequence, antibacterial peptide from crocodile (Crocodylus siamensis) white blood cell extracts.</i> The Journal of Antibiotics, <i>67</i>(3), 205–212. https://doi.org/10.1038/ja.2013.114
| + | |
| − | </li>
| + | |
| − | <li>
| + | |
| − | <a href="http://www.newpharma.be/pharmacie/sanofi-aventis-belgium/980/chloramine-60-comprimes.html">Newpharma. <i>Chloramine 60 Comprimés de SANOFI AVENTIS BELGIUM.</i> </a>
| + | |
| − | </li>
| + | |
| − | </ol>
| + | |
| − | </section>
| + | |
| − | | + | |
| − | | + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="category-content" id="2">
| + | |
| − | <div class="summary-left">
| + | |
| − | <ul>
| + | |
| − | <li data-number="1" style="list-style-type: none;">Business Plan</li>
| + | |
| − | <li data-number="2">1- Status and organization</li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | <div class="content-right">
| + | |
| − | <section id="bp1">
| + | |
| − | <h1>Business Plan</h1>
| + | |
| − | <p> | + | |
| − | Now that we have defined in more detail the prototype of our product, we wanted to carry out the entrepreneurship approach by making a complete business plan. A prerequisite to starting a new business is to analyze the market and find the best way to get into it. We would like to warmly thank Mr. Hoffmann, deputy director of CRITT Bio-Industries who helped us in the development of this business plan. He was able to enlighten us on certain points thanks to his expertise in the creation and development of companies in the field of biotechnology processes.
| + | |
| − | </p>
| + | |
| | </section> | | </section> |
| | | | |
| − | <section id="bp2"> | + | <section> |
| − | <h1>Chapter I - Status and organization </h1> | + | <h1>Membrane permeability assay</h1> |
| − | <h2> Legal status</h2>
| + | <p>We assessed the capacity of our microorganism-impermeable materials to let AMP go through. </p> |
| − | <p> | + | |
| − | Our project would be developed at first as a start-up. Some legal statuses are more adapted to start-ups than others. The french status SARL (Société A Responsabilité Limitée, corresponding to Limited Liability Company) has some drawbacks: the capital is shared in social parts, without any distinctions of profiles. New associates cannot simply join the social parts: complex procedures are needed. The status is not enough flexible for the creation of a start-up.
| + | |
| − | </p>
| + | |
| − | <p>
| + | |
| − | In contrast, the status of SAS (Société par Actions Simplifiée, which has no english equivalent but could be translated as a “simplified limited liability company”) seems much more flexible and suitable to start-ups. Different social parts associated with different rights are available, the governance body can be modified and finally, there are lots of liberties for status definitions as well as for the arrival of new associates.
| + | |
| − | </p>
| + | |
| | </section> | | </section> |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| | | | |
| | | | |
| Line 408: |
Line 113: |
| | | | |
| | <!-- C O N T E N T --> | | <!-- C O N T E N T --> |
| − | <script type="text/javascript">
| |
| − | $(document).on("click",".pe-category-inside", function(){
| |
| − | var target = $(this).data("target");
| |
| − | $('.pe-category-inside').removeClass('category-selected');
| |
| − | $(this).addClass('category-selected');
| |
| − | $('.category-content').removeClass("visible");
| |
| − | $("#"+target).addClass("visible");
| |
| − | console.log("target: "+target+ "pos: "+($("#"+target).position().top));
| |
| − | // Smooth scroll to the category
| |
| − | var c_0 = $("#"+target).position().top-70;
| |
| − | $('.main_content').animate({scrollTop: c_0 }, 1000);
| |
| − |
| |
| − | });
| |
| − | </script>
| |
| | | | |
| − | <script type="text/javascript">
| |
| − | $('.main_content').scroll(function(){
| |
| − | var pos = $('.main_content').scrollTop();
| |
| − | // Scope Statement
| |
| − | if($('*[data-target="1"]').hasClass('category-selected')){
| |
| − | if(pos>$("#sc1").position().top-70){
| |
| − | $('.summary-left ul li').css("font-weight", "normal");
| |
| − | $('*[data-number="1"]').css("font-weight", "bold");
| |
| − | if(pos>$("#sc2").position().top-70){
| |
| − | $('.summary-left ul li').css("font-weight", "normal");
| |
| − | $('*[data-number="2"]').css("font-weight", "bold");
| |
| − | if(pos>$("#sc3").position().top-70){
| |
| − | $('.summary-left ul li').css("font-weight", "normal");
| |
| − | $('*[data-number="3"]').css("font-weight", "bold");
| |
| − | if(pos>$("#sc4").position().top-70){
| |
| − | $('.summary-left ul li').css("font-weight", "normal");
| |
| − | $('*[data-number="4"]').css("font-weight", "bold");
| |
| − | }
| |
| − | }
| |
| − | }
| |
| − | }
| |
| − | // FIXED ASIDE NAV
| |
| − | if(pos>$("#sc1").position().top){
| |
| − | $('.summary-left').addClass("summary-fixed");
| |
| − | }
| |
| − | else{
| |
| − | $('.summary-left').removeClass("summary-fixed");
| |
| − | }
| |
| − | }
| |
| − | });
| |
| − | </script>
| |
| | | | |
| | </html> | | </html> |
| | {{INSA-UPS_France/General_script}} | | {{INSA-UPS_France/General_script}} |
| | {{INSA-UPS_France/Header_script}} | | {{INSA-UPS_France/Header_script}} |
Croc'n Cholera
