Difference between revisions of "Team:Lethbridge HS/Part Collection"

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<h1><b>Basic Parts:</b></h1>
 
<h1><b>Basic Parts:</b></h1>
 
<br>
 
<br>
 +
 +
 +
<h1><b>Composite Parts</b></h1>
 +
<br>
 +
<h2><b>&nbsp;Anthocyanin Constructs</b></h2>
 
<br>
 
<br>
−
<h2><b>Anthocyanin</b></h2>
+
<h3>Anthocyanin composite part 1</h3>
−
<h3><i>3gt</i></h3>
+
<p class="center">
−
<p class="center"> The gene <i>3gt</i> is from the anthocyanin synthesis pathway and converts the initial molecule Pelargonidin into Anthocyanin. This gene is from the organism <i>Petunia hybrid</i>. We have added this part to the registry as one of our new basic part submissions. Part BBa_idontknowwewillfindout
+
<a href="">BBa_K1281101</a>
−
</p>
+
 
<br>
 
<br>
−
<h3><i>yad</i>H</h3>
+
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
−
<p class="center">This Gene is an <i>Escherichia coli</i> gene that has been shown to increase the yields of anthocyanin when paired with the genes in our anthocyanin construct. It is our second original basic part submission to the registry. It is uncharacterized and is an inter-membrane transport protein. Part BBa_wowzers.
+
−
</p>
+
 
<br>
 
<br>
−
<h3><i>f3h</i></h3>
+
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
−
<p class="center">We will be useing the gene <i>f3h</i> as the first gene in our anthocyanin synthesis pathway, it comes from the organism <i>Petroselinum crispum</i>. It was added to the registry by the 2014 Darmstadt iGEM team, part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>. This gene will code for a protein that converts the initial molecule flavanone into dihydroflavonol.
+
−
</p>
+
 
<br>
 
<br>
−
<h3><i>dfr</i></h3>
+
<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>
−
<p class="center">The gene <i>dfr</i> is the second one in our anthocyanin synthesis pathway. We are using the biobrick part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>. It was added to the registry by the 2014 Darmstadt igem team. </p>
+
 
<br>
 
<br>
−
<h3><i>ans</i></h3>
+
<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>
−
<p class="center">This gene is the third gene in our pathway, it converts the molecule created by dfr into pelargonidin. It is from the organism <i>Fragaria x ananassa</i> and was added to the registry by the 2014 Darmstadt team. It is an engineered anthocyanidin synthase. Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>.
+
−
</p>
+
 
<br>
 
<br>
−
<br>
+
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
−
<h2><b>Zeaxanthin</b></h2>
+
−
<h3><i>crt</i>Y</h3>
+
−
<p class="center">This gene is from the organism <i>Pantoea ananatis</i> and is part or the carotenoid synthesis pathway. It converts the initial molecule Lycopene into the final molecule Beta-Carotene. This gene was added to the registry by Edinburgh 2007, part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I742154">BBa_I742154</a>.  
+
 
</p>
 
</p>
 
<br>
 
<br>
−
<h3><i>crtZ</i></h3>
+
 
−
<p class="center">This gene is from the organism <i>Pantoea ananatis</i> and is our final gene in the carotenoid synthesis pathway. It was added to the registry by Edinburgh 2007 and is the part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I742157">BBa_I742157</a>.This part converts the Beat-Carotene into our final product, the pigment Zeaxanthin.
+
<h3>Anthocyanin composite part 2</h3>
−
</p>
+
<p class="center">
 +
<a href="">BBa_K1281102</a>
 
<br>
 
<br>
−
<h2><b>Melanin</b></h2>
+
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
−
<h3><i>mel</i>A</h3>
+
−
<p class="center">The gene melA is from the organism <i>Rhizobium etli</i> and was added to the registry by the Cambridge 2009 iGEM team. Part <a href="http://parts.igem.org/Part:BBa_K274001">BBa_K274001</a>.
+
−
</p>
+
 
<br>
 
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<h2><b>Indigoidine</b></h2>
+
<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>
−
<h3><i>ind</i>B</h3>
+
−
<p class="center">This gene is from the organism <i>Streptomyces chrmofuscus</i>, and it is our third original basic part submission to the registry. Its is part BBa_funthings. It has been shown to increase the yeilds of Indigoidine when used with indC. It is a putative phosphatase.
+
−
</p>
+
 
<br>
 
<br>
−
<h3><i>ind</i>C</h3>
+
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
−
<p class="center">This gene is the gene that converts Glutamine into Indigoidine. It is from the organism <i>Photohabdus luminescens</i> and was added to the registry by the 2013 Heidelburg iGEM team. Part <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a>.
+
 
</p>
 
</p>
 
<br>
 
<br>
−
<br>
 
−
<h2><b>Additional  Basic parts</b></h2>
 
−
<h3>T7 Promoter</h3>
 
−
<p class="center">The Promoter we used for our Melanin Construct is  T7 promoter, This allows us to control the production of Melanin. This promoter is from the T7 bacteriophage, it is a Virus that inserts its DNA into Bacteria in order to reproduce and stay alive. This promoter works with the T7 system inside the <i>Escherichia coli</i> strain BL21(DE3), (Fig 1.)
 
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<br>
 
−
<br>
 
−
<img src="https://static.igem.org/mediawiki/2017/d/dc/T--Lethbridge_HS--T7System.png" class="img-responsive" >
 
−
<br>
 
−
(Fig 1.) This image shows the T7 Promoter system, 1.Lactose inducible promoter 2.<i>E. coli</i> Ribosomal Binding site. 3.The Gene for T7 RNA polymerase. 4.Terminator 5.T7 RNA polymerase. 6.T7 promoter. 7.<i>E. coli</i> Ribosomal Binding site. 8.The gene/genes in our construct. 9.Terminator. 10.mRNA produced by the transcription of the construct.
 
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<br>
 
−
This shows the process that occurs with the T7 System. We induce the Lactose inducible promoter(1) with IPTG as it mimics the lactose and cannot be broken down by the cell. This allows it to continually perform transcription on the construct(1-4) embedded in the Genome of the BL21(DE3) and thusly constantly produce the T7 RNA polymerase(5) encoded by the gene(3).This T7 RNA Polymerase(5) attaches to the T7 Promoter(6) and starts transcription on the construct(6-9) within our plasmid psB1C3. This then creates the mRNA(10) which will undergo translation and produce the protein encoded by the gene(8) due to the <i>E. coli</i> RBS on the construct. Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
−
</p>
 
−
<br>
 
−
<h3>Ribosomal Binding Site</h3>
 
−
<p class="center">The RBS we have chosen for our constructs is an <i>E. coli</i> Ribosomal Binding Site, Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>
 
−
</p>
 
−
<br>
 
−
<h3>Terminator</h3>
 
−
<p class="center">The terminator we have chosen for our constructs is an <i>E. coli</i> terminator. Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>, it is a double terminator made up of one <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0012">BBa_B0012</a> terminator and a <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0010">BBa_B0010</a> terminator.
 
−
</p>
 
−
<br>
 
−
<br>
 
−
<br>
 
−
<h1><b>Composite Parts:</b></h1>
 
−
 
  
−
<h3><b>Anthocyanin Construct</b></h3>
+
<h3>Anthocyanin composite part 3</h3>
−
<p class="center"> This construct is being used to convert our initial molecule Eriodictyol into our final molecule Anthocyanin. The genes are:
+
<p class="center">
 +
<a href="">BBa_K1281103</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>
+
<b><i>3gt</i></b> Part <a href="">BBa_K1281003</a>
 
<br>
 
<br>
−
<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>
+
<b><i>yad</i>H</b> Part <a href="">BBa_K1281002</a>
 
<br>
 
<br>
−
<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>
+
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
<b><i>3gt</i></b> Part BBa_waitingforsubmission
 
 
<br>
 
<br>
−
<b><i>yad</i>H</b> Part BBa_alsowaiting
+
These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted
 
<br>
 
<br>
−
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
+
 
 +
 
 +
<div style="text-align:center;"><center><img height=50px class="img-responsive"src="https://static.igem.org/mediawiki/2017/1/15/T--Lethbridge_HS--AnthocyaninPathway.png"></center></div>
 
<br>
 
<br>
−
These genes will all be used in the cell to produce Anthocyanin out of Eriodictyol. The <i>yad</i>H has been shown to increase yields even though it is not directly involved in the pathway. The <i>3gt</i> as well as <i>yad</i> H are two of our original basic part submissions. This Construct is our composite part submission BBa_anthocyanincompsitepart.
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/4/49/T--Lethbridge_HS--Anthocyanincomp1.png"></center></div>
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/1/15/T--Lethbridge_HS--AnthocyaninPathway.png"></div>
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/9/9f/T--Lethbridge_HS--Anthocyanincomp2.png"></center>
−
<br>
+
</div>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/2/24/T--Lethbridge_HS--AnthocyaninConstruct.png"></div>
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/0c/T--Lethbridge_HS--Anthocyanincomp3.png"></center>
−
<br>
+
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/b/b4/T--Lethbridge_HS--yadHconstruct.png">
+
 
</div>
 
</div>
 
</p>
 
</p>
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<br>
 
<br>
  
−
<h3><b>Zeaxanthin Construct</b></h3>
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<h2><b>&nbsp;Zeaxanthin Construct</b></h2>
−
<p class="center"> This construct is made up of the following genes:
+
<h3>Zeaxanthin composite part</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281301</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
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<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
These parts all come together in our composite part <!-- link to the part--> BBa_Zeaxanthinconstruct. This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.
 
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/8/8f/T--Lethbridge_HS--ZeaxanthinPathway.png">
+
This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.
 +
<br>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/8/8f/T--Lethbridge_HS--ZeaxanthinPathway.png"></center>
 
</div>
 
</div>
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/c/c5/T--Lethbridge_HS--ZeaxanthinConstruct.png">
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/c/c5/T--Lethbridge_HS--ZeaxanthinConstruct.png"></center>
 
</div>
 
</div>
 
</p>
 
</p>
 
<br>
 
<br>
 +
 +
<h2><b>&nbsp;Indigoidine Constructs</b></h2>
 +
<h3>Indigoidine composite part 1</h3>
 +
<p class="center">
 +
<a href="">BBa_K1281201</a>
 +
<br>
 +
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 +
<br>
 +
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 +
<br>
 +
<b><i>ind</i>B</b> Part <a href="">BBa_K1281001</a>
 +
<br>
 +
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 +
</p>
 
<br>
 
<br>
  
−
<h3><b>Indigoidine Construct</b></h3>
+
<h3>Indigoidine composite part 2</h3>
−
<p class="center">Our Indigoidine construct is made up of:
+
<p class="center">
 +
<a href="">BBa_K1281202</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<br>
 
<br>
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
−
<br>
 
−
<b><i>ind</i>B</b> Part <!--waiting for our part submission here-->BBa_waiting for actual part submission.
 
 
<br>
 
<br>
 
<b><i>ind</i>C</b> Part <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a>
 
<b><i>ind</i>C</b> Part <a href="http://parts.igem.org/Part:BBa_K1152013">BBa_K1152013</a>
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<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
These two genes come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is one of our original basic part submissions. This construct is part BBa_idkyet
 
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png">
+
These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. This construct is part <a href="">BBa_K1281201</a> and part <a href="">BBa_K1281202</a>. We had to split our genes into tow separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.
 +
<br>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png"></center>
 
</div>
 
</div>
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/2/25/T--Lethbridge_HS--IndigoidineConstruct.png">
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/2/25/T--Lethbridge_HS--IndigoidineConstruct.png"></center>
 
</div>
 
</div>
 
</p>  
 
</p>  
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−
<h3><b>Melanin Construct</b></h3>
+
<h2><b>&nbsp;Melanin Construct</b></h2>
 +
<h3>Melanin composite part</h3>
 
<p class="center">The parts used in our Melanin construct are as follows:
 
<p class="center">The parts used in our Melanin construct are as follows:
 
<br>
 
<br>
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<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<br>
 
<br>
−
This construct allows us to make Melanin out of L-Tyrosine. it is Part BBa_waitandsee
 
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/a/ad/T--Lethbridge_HS--MelaninPathway.png">
+
This construct allows us to make Melanin out of L-Tyrosine.
 +
<br>
 +
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/a/ad/T--Lethbridge_HS--MelaninPathway.png"></center>
 
</div>
 
</div>
 
<br>
 
<br>
−
<div style="text-align:center;"><img class="img-responsive"src="https://static.igem.org/mediawiki/2017/f/f2/T--Lethbridge_HS--MelaninConstruct.png">
+
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/f/f2/T--Lethbridge_HS--MelaninConstruct.png"></center>
 
</div>
 
</div>
 
</p>
 
</p>
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Revision as of 22:33, 26 October 2017





Basic Parts:


Composite Parts


 Anthocyanin Constructs


Anthocyanin composite part 1

BBa_K1281101
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
dfr Part BBa_K1497010
f3h Part BBa_K1497009
Terminator Part BBa_B0015.


Anthocyanin composite part 2

BBa_K1281102
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
ans Part BBa_K1497002
Terminator Part BBa_B0015.


Anthocyanin composite part 3

BBa_K1281103
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
3gt Part BBa_K1281003
yadH Part BBa_K1281002
Terminator Part BBa_B0015.

These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted





 Zeaxanthin Construct

Zeaxanthin composite part

BBa_K1281301
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
crtY Part BBa_I742154.
crtZ Part BBa_I742157.
Terminator Part BBa_B0015.

This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.



 Indigoidine Constructs

Indigoidine composite part 1

BBa_K1281201
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indB Part BBa_K1281001
Terminator Part BBa_B0015.


Indigoidine composite part 2

BBa_K1281202
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indC Part BBa_K1152013
Terminator Part BBa_B0015.

These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The indB has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. This construct is part BBa_K1281201 and part BBa_K1281202. We had to split our genes into tow separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.




 Melanin Construct

Melanin composite part

The parts used in our Melanin construct are as follows:
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
MelA Part BBA_K274001.
Terminator Part BBa_B0015.

This construct allows us to make Melanin out of L-Tyrosine.