Difference between revisions of "Team:Exeter/Basic Part"

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           <p>The sfGFP was inserted in three different places in the FimH protein to act as a reporter on the level of expression of the protein and thus giving an indication of its efficiency. The three places of insertion were 22, which is the place where the signal peptide ends in the mannose binding domain, the 225 and 258, which are present in the domain that interacts with FimG</p>
 
           <p>The sfGFP was inserted in three different places in the FimH protein to act as a reporter on the level of expression of the protein and thus giving an indication of its efficiency. The three places of insertion were 22, which is the place where the signal peptide ends in the mannose binding domain, the 225 and 258, which are present in the domain that interacts with FimG</p>
  
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<table style="width:90%; margin:0 auto; border-collapse: collapse;  border: 2px solid black" border="1" >
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  <tr>
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<th>Name</th>
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<th>Description</th>
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<th>Base Pairs</th>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2324002">BBa_K2324002</a></td>
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<td>FimH_22_sfGFP</td>
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<td>1617</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2324001">BBa_K2324001</a></td>
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<td>FimH_225_sfGFP</td>
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<td>1617</td>
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  </tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2324003">BBa_K2324003</td>
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<td>FimH_258_sfGFP</td>
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<td>1617</td>
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  </tr>
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</table>
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</div >
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<div style="width:90%;
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height:auto ;margin: 30px auto; clear:left">
 
         <h4 id="h2">FimH Fusion Proteins</h4>
 
         <h4 id="h2">FimH Fusion Proteins</h4>
 
           <p>The metal binding proteins were inserted at the 22nd residue of the FimH protein, because that is where the siganl peptide of FimH ends and it is part of the mannose binding domain. Whereas, the 225 and 258 inserts are in the domain that interacts with FimG.</p>  
 
           <p>The metal binding proteins were inserted at the 22nd residue of the FimH protein, because that is where the siganl peptide of FimH ends and it is part of the mannose binding domain. Whereas, the 225 and 258 inserts are in the domain that interacts with FimG.</p>  

Revision as of 17:28, 27 October 2017

FimH sfGFP

The sfGFP was inserted in three different places in the FimH protein to act as a reporter on the level of expression of the protein and thus giving an indication of its efficiency. The three places of insertion were 22, which is the place where the signal peptide ends in the mannose binding domain, the 225 and 258, which are present in the domain that interacts with FimG

Name Description Base Pairs
BBa_K2324002 FimH_22_sfGFP 1617
BBa_K2324001 FimH_225_sfGFP 1617
BBa_K2324003 FimH_258_sfGFP 1617

FimH Fusion Proteins

The metal binding proteins were inserted at the 22nd residue of the FimH protein, because that is where the siganl peptide of FimH ends and it is part of the mannose binding domain. Whereas, the 225 and 258 inserts are in the domain that interacts with FimG.

Fim Operon

The fim operon consists of six proteins with a native RBS.