Difference between revisions of "Team:TCFSH Taiwan/Composite Part"

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<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.1.0/jquery.min.js"></script>
 
<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.1.0/jquery.min.js"></script>
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<link href="https://2016.igem.org/Team:NCTU_Formosa/wiki_reset?action=raw&ctype=text/css" rel="stylesheet">
 
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300,400" rel="stylesheet">
 
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300,400" rel="stylesheet">
  
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     text-indent:2em;
 
     text-indent:2em;
 
     color:#F3F7F7;
 
     color:#F3F7F7;
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    padding-left:40px;
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    padding-top:15px;
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    padding-bottom:15px;
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}
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.content-1{
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    font-size:10pt !important;
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    text-align:justify;
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    text-indent:2em;
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    color:#993366;
 
     padding-left:40px;
 
     padding-left:40px;
 
     padding-top:15px;
 
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<img src="https://static.igem.org/mediawiki/2017/b/b8/Composite1.jpeg" class="bigphoto" width=70%>
 
<img src="https://static.igem.org/mediawiki/2017/b/b8/Composite1.jpeg" class="bigphoto" width=70%>
  
<p class="content">R0011 (Promoter) + B0034 (RBS) +BBa_k2383001 + B0034 (RBS) +BBa_K1725420 (UirR) + BBa_K1725410 (UirS)</p>
+
<p class="content-1">R0011 (Promoter) + B0034 (RBS) +BBa_k2383001 + B0034 (RBS) +BBa_K1725420 (UirR) + BBa_K1725410 (UirS)</p>
  
 
<!--compositepart 2-->
 
<!--compositepart 2-->
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<img src="https://static.igem.org/mediawiki/2017/0/0a/Composite2.jpeg" class="bigphoto" width=70%>
 
<img src="https://static.igem.org/mediawiki/2017/0/0a/Composite2.jpeg" class="bigphoto" width=70%>
  
<p class="content">J23101 (Promoter) + BBa_K115002 (RBS) + BBa_K115002 + BBa_C0171 (RhlR) + BBa_K1541017 (RhlI)</p>
+
<p class="content-1">J23101 (Promoter) + BBa_K115002 (RBS) + BBa_K115002 + BBa_C0171 (RhlR) + BBa_K1541017 (RhlI)</p>
  
 
<!--compositepart 3-->
 
<!--compositepart 3-->
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<img src="https://static.igem.org/mediawiki/2017/3/38/Composite3.jpeg" class="bigphoto" width=70%>
 
<img src="https://static.igem.org/mediawiki/2017/3/38/Composite3.jpeg" class="bigphoto" width=70%>
  
<p class="content">B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)</p>
+
<p class="content-1">B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)</p>
  
 
<!--compositepart 4-->
 
<!--compositepart 4-->
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<p class="content">This is a Report system of UV with LacI. The biobrick will be like:</p>
 
<p class="content">This is a Report system of UV with LacI. The biobrick will be like:</p>
  
<img src="https://static.igem.org/mediawiki/2017/3/38/Composite4.jpeg" class="bigphoto" width=70%>
+
<img src="https://static.igem.org/mediawiki/2017/a/a1/Composite4.jpeg" class="bigphoto" width=70%>
  
<p class="content">B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)</p>
+
<p class="content-1">B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)</p>
  
  

Revision as of 15:59, 28 October 2017

Composite Parts

We have know the function of two basic parts, the cjBlue-LVA and LacI without LVA. Hence, we create biobrick using the basic parts we mentioned and other different parts which will be mentioned below.

Composite BBa_K2383100

We us the cjBlue_LVA in this part, and add UirR, UirS into it. The biobrick will be like:

R0011 (Promoter) + B0034 (RBS) +BBa_k2383001 + B0034 (RBS) +BBa_K1725420 (UirR) + BBa_K1725410 (UirS)

Composite BBa_K2383200

This is a RhlR Regulating system with RNA thermometer. The biobrick will be like:

J23101 (Promoter) + BBa_K115002 (RBS) + BBa_K115002 + BBa_C0171 (RhlR) + BBa_K1541017 (RhlI)

Composite BBa_K2383300

This is a Report system of UV and Temperature with LacI. The biobrick will be like:

B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)

Composite BBa_K2383301

This is a Report system of UV with LacI. The biobrick will be like:

B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)