Difference between revisions of "Team:Lethbridge HS/Composite Part"

Line 79: Line 79:
 
<h3>Anthocyanin composite part 1</h3>
 
<h3>Anthocyanin composite part 1</h3>
 
<p class="center">
 
<p class="center">
−
<a href="">BBa_K1281101</a>
+
<a href="">BBa_K2481113</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 85: Line 85:
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497010">BBa_K1497010</a>
+
<b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481110">BBa_K2481110</a>
 
<br>
 
<br>
−
<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497009">BBa_K1497009</a>
+
<b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481111">BBa_K2481111</a>
 
<br>
 
<br>
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
Line 95: Line 95:
 
<h3>Anthocyanin composite part 2</h3>
 
<h3>Anthocyanin composite part 2</h3>
 
<p class="center">
 
<p class="center">
−
<a href="">BBa_K1281102</a>
+
<a href="">BBa_K2481114</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 101: Line 101:
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K1497002">BBa_K1497002</a>
+
<b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481112">BBa_K2481112</a>
 
<br>
 
<br>
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
Line 109: Line 109:
 
<h3>Anthocyanin composite part 3</h3>
 
<h3>Anthocyanin composite part 3</h3>
 
<p class="center">
 
<p class="center">
−
<a href="">BBa_K1281103</a>
+
<a href="">BBa_K2481105</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 115: Line 115:
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>3gt</i></b> Part <a href="">BBa_K1281003</a>
+
<b><i>3gt</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481002">BBa_K2481002</a>
 
<br>
 
<br>
−
<b><i>yad</i>H</b> Part <a href="">BBa_K1281002</a>
+
<b><i>yad</i>H</b> Part <a href="http://parts.igem.org/Part:BBa_K2481004">BBa_K2481004</a>
 
<br>
 
<br>
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
Line 141: Line 141:
 
<h3>Zeaxanthin composite part</h3>
 
<h3>Zeaxanthin composite part</h3>
 
<p class="center">
 
<p class="center">
−
<a href="">BBa_K1281301</a>
+
<a href="http://parts.igem.org/Part:BBa_K2481107">BBa_K2481107</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 167: Line 167:
 
<h3>Indigoidine composite part 1</h3>
 
<h3>Indigoidine composite part 1</h3>
 
<p class="center">
 
<p class="center">
−
<a href="">BBa_K1281201</a>
+
<a href="http://parts.igem.org/Part:BBa_K2481106">BBa_K2481106</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 173: Line 173:
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>.
 
<br>
 
<br>
−
<b><i>ind</i>B</b> Part <a href="">BBa_K1281001</a>
+
<b><i>ind</i>B</b> Part <a href="http://parts.igem.org/Part:BBa_K2481001">BBa_K2481001</a>
 
<br>
 
<br>
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
 
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>.
Line 181: Line 181:
 
<h3>Indigoidine composite part 2</h3>
 
<h3>Indigoidine composite part 2</h3>
 
<p class="center">
 
<p class="center">
−
<a href="">BBa_K1281202</a>
+
<a href="http://parts.igem.org/Part:BBa_K2481109">BBa_K2481109</a>
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
Line 206: Line 206:
 
<h2><b>&nbsp;Melanin Construct</b></h2>
 
<h2><b>&nbsp;Melanin Construct</b></h2>
 
<h3>Melanin composite part</h3>
 
<h3>Melanin composite part</h3>
−
<p class="center">The parts used in our Melanin construct are as follows:
+
<p class="center">
 +
<a href="http://parts.igem.org/Part:BBa_K2481108">BBa_K2481108</a.
 
<br>
 
<br>
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.
 
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>.

Revision as of 17:50, 28 October 2017





 Anthocyanin Constructs


Anthocyanin composite part 1

BBa_K2481113
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
dfr Part BBa_K2481110
f3h Part BBa_K2481111
Terminator Part BBa_B0015.


Anthocyanin composite part 2

BBa_K2481114
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
ans Part BBa_K2481112
Terminator Part BBa_B0015.


Anthocyanin composite part 3

BBa_K2481105
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
3gt Part BBa_K2481002
yadH Part BBa_K2481004
Terminator Part BBa_B0015.

These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted





 Zeaxanthin Construct

Zeaxanthin composite part

BBa_K2481107
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
crtY Part BBa_I742154.
crtZ Part BBa_I742157.
Terminator Part BBa_B0015.

This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.



 Indigoidine Constructs

Indigoidine composite part 1

BBa_K2481106
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indB Part BBa_K2481001
Terminator Part BBa_B0015.


Indigoidine composite part 2

BBa_K2481109
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indC Part BBa_K1152013
Terminator Part BBa_B0015.

These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The indB has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. This construct is part BBa_K1281201 and part BBa_K1281202. We had to split our genes into tow separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.




 Melanin Construct

Melanin composite part

BBa_K2481108 T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
MelA Part BBA_K274001.
Terminator Part BBa_B0015.

This construct allows us to make Melanin out of L-Tyrosine.