Difference between revisions of "Team:Lethbridge HS/Contribution"
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<br> <br> <br> | <br> <br> <br> | ||
<div class="container-fluid" style="margin-left:10%; margin-right:10%;"> | <div class="container-fluid" style="margin-left:10%; margin-right:10%;"> | ||
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<h1><b> Basic Parts</b></h1> | <h1><b> Basic Parts</b></h1> | ||
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<br> | <br> | ||
<h3><i>ind</i>B</h3> | <h3><i>ind</i>B</h3> | ||
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<h1><b> Composite Parts</b></h1> | <h1><b> Composite Parts</b></h1> | ||
<br> | <br> | ||
| − | + | <br> | |
| − | <h2><b> Anthocyanin Constructs</b></h2> | + | <div class="container-fluid" style="margin-left:10%; margin-right:10%;"> <h2><b> Anthocyanin Constructs</b></h2> |
<br> | <br> | ||
<h3>Anthocyanin composite part 1</h3> | <h3>Anthocyanin composite part 1</h3> | ||
<p class="center"> | <p class="center"> | ||
| − | <a href=""> | + | <a href="">BBa_K2481113</a> |
<br> | <br> | ||
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | <b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | ||
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<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. | <b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. | ||
<br> | <br> | ||
| − | <b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part: | + | <b><i>dfr</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481110">BBa_K2481110</a> |
<br> | <br> | ||
| − | <b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part: | + | <b><i>f3h</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481111">BBa_K2481111</a> |
<br> | <br> | ||
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>. | <b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>. | ||
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<h3>Anthocyanin composite part 2</h3> | <h3>Anthocyanin composite part 2</h3> | ||
<p class="center"> | <p class="center"> | ||
| − | <a href=""> | + | <a href="">BBa_K2481114</a> |
<br> | <br> | ||
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | <b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | ||
| Line 134: | Line 137: | ||
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. | <b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. | ||
<br> | <br> | ||
| − | <b><i>ans</i></b> Part <a href="http://parts.igem.org/Part: | + | <b><i>ans</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481112">BBa_K2481112</a> |
<br> | <br> | ||
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>. | <b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>. | ||
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<h3>Anthocyanin composite part 3</h3> | <h3>Anthocyanin composite part 3</h3> | ||
<p class="center"> | <p class="center"> | ||
| − | <a href=""> | + | <a href="http://parts.igem.org/Part:BBa_K2481105">BBa_K2481105</a> |
<br> | <br> | ||
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | <b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | ||
| Line 148: | Line 151: | ||
<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. | <b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. | ||
<br> | <br> | ||
| − | <b><i>3gt</i></b> Part <a href=""> | + | <b><i>3gt</i></b> Part <a href="http://parts.igem.org/Part:BBa_K2481002">BBa_K2481002</a> |
<br> | <br> | ||
| − | <b><i>yad</i>H</b> Part <a href=""> | + | <b><i>yad</i>H</b> Part <a href="http://parts.igem.org/Part:BBa_K2481004">BBa_K2481004</a> |
<br> | <br> | ||
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>. | <b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>. | ||
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<h3>Zeaxanthin composite part</h3> | <h3>Zeaxanthin composite part</h3> | ||
<p class="center"> | <p class="center"> | ||
| − | <a href=""> | + | <a href="http://parts.igem.org/Part:BBa_K2481107">BBa_K2481107</a> |
<br> | <br> | ||
<b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | <b>T7 Promoter</b> Part <a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | ||
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<h3>Indigoidine composite part 1</h3> | <h3>Indigoidine composite part 1</h3> | ||
<p class="center"> | <p class="center"> | ||
| − | <a href=""> | + | <a href="http://parts.igem.org/Part:BBa_K2481106">BBa_K2481106</a> |
<br> | <br> | ||
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | <b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | ||
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<b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. | <b>RBS</b> Part <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. | ||
<br> | <br> | ||
| − | <b><i>ind</i>B</b> Part <a href=""> | + | <b><i>ind</i>B</b> Part <a href="http://parts.igem.org/Part:BBa_K2481001">BBa_K2481001</a> |
<br> | <br> | ||
<b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>. | <b>Terminator</b> Part <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>. | ||
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<h3>Indigoidine composite part 2</h3> | <h3>Indigoidine composite part 2</h3> | ||
<p class="center"> | <p class="center"> | ||
| − | <a href=""> | + | <a href="http://parts.igem.org/Part:BBa_K2481109">BBa_K2481109</a> |
<br> | <br> | ||
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | <b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | ||
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<br> | <br> | ||
<br> | <br> | ||
| − | These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions | + | These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The <i>ind</i>B has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. We had to split our genes into two separate composite part submissions as the size of each was too large to allow for them to be in one plasmid. |
<br> | <br> | ||
<div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png"></center> | <div style="text-align:center;"><center><img height="150px"class="img-responsive"src="https://static.igem.org/mediawiki/2017/0/02/T--Lethbridge_HS--IndigoidinePathway.png"></center> | ||
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<h2><b> Melanin Construct</b></h2> | <h2><b> Melanin Construct</b></h2> | ||
<h3>Melanin composite part</h3> | <h3>Melanin composite part</h3> | ||
| − | <p class="center"> | + | <p class="center"> |
| + | <a href="http://parts.igem.org/Part:BBa_K2481108">BBa_K2481108</a> | ||
<br> | <br> | ||
<b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | <b>T7 Promoter</b> Part<a href="http://parts.igem.org/Part:BBa_I712074">BBa_I712074</a>. | ||
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Revision as of 18:01, 28 October 2017
Basic Parts
indB
BBa_K1281001
This Gene is from the organism Petunia hybrid. It has been shwo to increase the yeilds of Indigoidine when paired with the gene indC. We could not separate this gene from its composite part so we have submitted the nucleotide sequence to the registry.
3gt
BBa_K281003
The gene 3gt is from the anthocyanin synthesis pathway and converts the initial molecule Pelargonidin into Anthocyanin. This gene is from the organism Petunia hybrid. We have added this part to the registry as one of our new basic part submissions. We could not get this gene out of its composite part so we added the sequence to the registry.
yadH
BBa_K1281002
This Gene is an Escherichia coli gene that has been shown to increase the yields of anthocyanin when paired with the genes in our anthocyanin construct. It is our second original basic part submission to the registry. It is uncharacterized and is an inter-membrane transport protein. We could not separate this gene form its composite part so we have added the nucleotide sequence to the registry
Composite Parts
Anthocyanin Constructs
Anthocyanin composite part 1
BBa_K2481113
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
dfr Part BBa_K2481110
f3h Part BBa_K2481111
Terminator Part BBa_B0015.
Anthocyanin composite part 2
BBa_K2481114
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
ans Part BBa_K2481112
Terminator Part BBa_B0015.
Anthocyanin composite part 3
BBa_K2481105
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
3gt Part BBa_K2481002
yadH Part BBa_K2481004
Terminator Part BBa_B0015.
These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted
Zeaxanthin Construct
Zeaxanthin composite part
BBa_K2481107
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
crtY Part BBa_I742154.
crtZ Part BBa_I742157.
Terminator Part BBa_B0015.
This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.
Indigoidine Constructs
Indigoidine composite part 1
BBa_K2481106
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indB Part BBa_K2481001
Terminator Part BBa_B0015.
Indigoidine composite part 2
BBa_K2481109
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indC Part BBa_K1152013
Terminator Part BBa_B0015.
These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The indB has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. We had to split our genes into two separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.
Melanin Construct
Melanin composite part
BBa_K2481108
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
MelA Part BBA_K274001.
Terminator Part BBa_B0015.
This construct allows us to make Melanin out of L-Tyrosine.
