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Revision as of 00:02, 29 October 2017
Results
Test Kit Assessment
To begin designing and experimenting with a new device for E. coli detection, the WLC iGEM team wanted to test already marketed water test kits. Using both laboratory strain and wild type E. coli, the WLC iGEM team evaluated the reliability of six test kits while assessing other features of the kits. We found that while the kits were relatively accurate, they could not detect lab strain E. coli, and most required 48 hours for results. Below is a table summarizing our findings:
pETail4 and Lambda phage tail proteins
To continue with our new device, we obtained the pETail4 plasmid, which contains the genes for the Lambda Phage-tail proteins. The pETail4 plasmid has been used in past research to produce Lambda phage tails for determining the distribution of the LamB protein on the outer membrane of E. coli. The tail itself is composed of several smaller proteins. It has been proposed that the portion of the tail specific to LamB is the J-chain. Thus, we wanted to isolate this portion, or at least the tip of this portion. We verified the expression of the J-chain with an SDS-PAGE (below)
