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| − | <h2>COMMON Page</h2> | + | <h2>InterLab</h2> |
| | </div> | | </div> |
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| − | <div class="nj-box3">
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| − | <img class="img-responsive" alt="" src="https://static.igem.org/mediawiki/2017/0/03/T-worldshaper-XSHS-00004.jpeg" />
| + | |
| − | </div>
| + | |
| | <div class="nj-box4"> | | <div class="nj-box4"> |
| | <p> | | <p> |
| − | Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quas sit expedita, iusto repellendus cumque, officia architecto consequatur illo fuga eum sed ut autem eos voluptas. Nemo, a, rem! Atque quisquam aperiam eaque tenetur autem, soluta itaque omnis. Minus nesciunt, sint, animi illum quo ab voluptate esse delectus unde maiores iure, quasi a suscipit ipsam aliquid voluptatem. Perspiciatis eveniet, pariatur illum aut cum dolor neque consequatur error aliquid facilis in quasi temporibus assumenda tempore, doloremque autem saepe enim nihil. Voluptates asperiores ullam voluptate quas similique ratione quia hic, eum distinctio laboriosam, consectetur tempora voluptatibus optio natus cumque est necessitatibus dolore alias. | + | Aiming to help develop a reliable and detailed protocol to enable direct florescence data comparison between labs, we decided to collaborate with The Measurement Committee as a Interlab study participant. |
| | + | </p> |
| | + | <p> |
| | + | The Interlab study required us to follow a standard protocol including transformation, incubation, plate reader calibration, florescence standard curve obtainment, cell culture florescence measurement, etc ( Detailed protocol information can be found at https://2017.igem.org/Competition/InterLab_Study ). During the whole Interlab study process, everyone, instructed by the team PI, studied the protocol carefully and worked on providing accurate and reliable experiment data to The Measurement Committee. |
| | + | </p> |
| | + | <p>1.Transformation of the test devices and +/- control</p> |
| | + | <p> |
| | + | Unfortunately, we had an unexplained problem while attempting to transform cells with the extracted DNA from the Distribution Kit Plates. Luckily, we heard from iGEM team Worldshaper-XSHS that they have successfully obtained the transformed colony but was unable to continue with the protocol due to lack of a plate reader in their lab. We invited representatives from Worldshaper-XSHS to join our Interlab project, providing transformed colony and they accepted our request. (To learn more about our cooperation, check out our |
| | + | <a href="https://2017.igem.org/Team:Worldshaper-Nanjing/Collaborations">Collaboration page</a> |
| | + | .) |
| | + | </p> |
| | + | <p>2.OD600 reference point measurement</p> |
| | + | <p> |
| | + | We strictly followed the standard protocols provided by the Measurement Committee and turned off pathlength correction accordingly. |
| | + | </p> |
| | + | <div class="nj-box3"> |
| | + | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/3/39/T-ASTWS-China-c001.jpg" alt="" /> |
| | + | </div> |
| | + | <p>3.Fluorescein standard curve obtainment</p> |
| | + | <p> |
| | + | We measured the fluorescence of 4 replicates of dilution series of fluorescein in PBS buffer. The results are as shown below. |
| | </p> | | </p> |
| | + | <div class="nj-box3"> |
| | + | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/a/ab/T-ASTWS-China-c002.jpg" alt="" /> |
| | + | </div> |
| | + | <p>4.Cell measurement</p> |
| | <p> | | <p> |
| − | Lorem ipsum dolor sit amet, consectetur adipisicing elit. Veritatis expedita repellendus laboriosam aliquid. Neque doloribus ea, id reprehenderit alias saepe debitis eligendi molestias odit, nesciunt rem. Dolorem saepe, provident dolore nesciunt laudantium nostrum enim natus veritatis harum maxime et iure ratione, nulla. Minus excepturi commodi tempore voluptate. Blanditiis similique dolor asperiores ex excepturi perspiciatis, dolores id esse. Voluptate beatae nesciunt cum esse ratione officiis necessitatibus blanditiis ea, laboriosam fugit vero maxime? Voluptatum illo dolorum autem pariatur quisquam. Voluptates soluta culpa necessitatibus veritatis tempora incidunt doloribus placeat repellat et facilis eum sapiente fugit numquam aut, laboriosam aspernatur, esse, magnam excepturi repudiandae amet voluptas nulla quidem. Veritatis nisi consequuntur saepe qui quisquam dignissimos assumenda, iusto odio. Dignissimos reprehenderit esse iusto cupiditate nisi enim, animi similique itaque, perspiciatis error qui. Aperiam, architecto provident. | + | We had correctly diluted our overnight transformed cell culture to a target OD600 of 0.02. Then we incubated the diluted culture and took samples at 0,2,4,6 hours after dilution and held them on ice. Then we measured the florescence of these samples and the results are as shown below. |
| | </p> | | </p> |
| | + | <div class="nj-box3"> |
| | + | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/8/8a/T-ASTWS-China-c003.jpg" alt="" /> |
| | + | </div> |
| | + | <p>Raw plate readings</p> |
| | + | <div class="nj-box3"> |
| | + | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/2/21/T-ASTWS-China-c004.jpg" alt="" /> |
| | + | </div> |
| | + | <p>Adjusted results</p> |
| | </div> | | </div> |
| | + | |
| | </div> | | </div> |
| | </div> | | </div> |
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| | </article> | | </article> |
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| | <footer id="footer"> | | <footer id="footer"> |
