Difference between revisions of "Team:Vilnius-Lithuania/Design"

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         <p>Transcript of RNA II forms a RNA-DNA duplex and acts as a primer for DNA polymerase and for that reason is often called replication initiator.</p>
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         <p>Transcript of <b>RNA II</b> forms a RNA-DNA duplex and acts as a primer for DNA polymerase and for that reason is often called <b>replication initiator</b>.</p>
 
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         <p>During the transcription of RNA II several different secondary structures can form. Part of the structures are susceptible to the binding of RNA I – a shorter antisense version of RNA II. The interaction between RNA I and RNA II start upon formation of kissing-loop pairs between their anti-complementary secondary structures. If the kissing complex persists 3’ end of RNA I starts forming a zipper-like duplex with complementary single strand RNA II region. This results in replication inhibition, because primer cannot be formed anymore, which is why RNA I is often called replication inhibitor.</p>
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         <p>During the transcription of RNA II several different secondary structures can form. Part of the structures are susceptible to the binding of RNA I – a shorter antisense version of RNA II. The interaction between RNA I and RNA II start upon formation of kissing-loop pairs between their anti-complementary secondary structures. If the kissing complex persists 3’ end of RNA I starts forming a zipper-like duplex with complementary single strand RNA II region. This results in replication inhibition, because primer cannot be formed anymore, which is why <b>RNA I is often called replication inhibitor</b>.</p>
  
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         <h2>H2 title</h2>
 
         <h2>H2 title</h2>

Revision as of 20:03, 30 October 2017

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Plasmid copy number control

Design

Flexible copy number control is the core of our framework, which is based on re-engineered ColE1 origin of replication.

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