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− | <p>The simulation results show that we can indeed reach a steady state concentration in the root area, and that this concentration depends on the enzyme concentration. This relationship is sigmoidal; on a semi-log graph, the linear region ranges between 10-7 mM and 10-6 mM of catalase concentration (Figure 1,2). The time required to approach steady state is reasonably short, at around 45 minutes (Figure 3). </p> | + | <p>The simulation results show that we can indeed reach a steady state concentration in the root area, and that this concentration depends on the enzyme concentration. This relationship is sigmoidal; on a semi-log graph, the linear region ranges between 10<sup>-7</sup> mM and 10<sup>-6</sup> mM of catalase concentration (Figure 1,2). The time required to approach steady state is reasonably short, at around 45 minutes (Figure 3). </p> |
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<img class = "img-responsive" src="https://static.igem.org/mediawiki/2017/f/fd/T--Cornell--transientstate.png" alt= "modelfigure3"> | <img class = "img-responsive" src="https://static.igem.org/mediawiki/2017/f/fd/T--Cornell--transientstate.png" alt= "modelfigure3"> | ||
− | <p>Figure 3: Mean peroxide concentration around the plant vs. time for 6 *10-10 M of catalase</p> | + | <p>Figure 3: Mean peroxide concentration around the plant vs. time for 6 *10<sup>-10</sup> M of catalase</p> |
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<img class = "img-responsive" src="https://static.igem.org/mediawiki/2017/6/67/SSPeroxideConcVaryingCatalase.png" alt= "modelfigure4"> | <img class = "img-responsive" src="https://static.igem.org/mediawiki/2017/6/67/SSPeroxideConcVaryingCatalase.png" alt= "modelfigure4"> | ||
− | <p>Figure 4: Distribution of steady state peroxide concentration across bacteria-plant system, varied by amount of catalase enzyme present (A: | + | <p>Figure 4: Distribution of steady state peroxide concentration across bacteria-plant system, varied by amount of catalase enzyme present (A: 10<sup>-8</sup> mM, B: 10<sup>-9</sup>mM, C: 10<sup>-10</sup> mM, D: 6*10<sup>-10</sup> mM, E: 6*10<sup>-11</sup> mM)</p> |
<div class="center"><embed width="38%" height="264" src="https://static.igem.org/mediawiki/2017/2/27/T--Cornell--transientmp4.mp4"></div> | <div class="center"><embed width="38%" height="264" src="https://static.igem.org/mediawiki/2017/2/27/T--Cornell--transientmp4.mp4"></div> | ||
− | <p>Figure 5: Distribution of peroxide concentration across bacteria-plant system over time for a steady-state catalase concentration of 6 *10<sup>-10 | + | <p>Figure 5: Distribution of peroxide concentration across bacteria-plant system over time for a steady-state catalase concentration of 6 *10<sup>-10 </sup> M.</p> |
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Latest revision as of 21:45, 30 October 2017
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