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<div> | <div> | ||
<h2 id="Assembly">Toolbox modules assembly</h2> | <h2 id="Assembly">Toolbox modules assembly</h2> | ||
− | <h4>How we constructed our circuits</h4> | + | <h4 style="padding: 0;">How we constructed our circuits</h4> |
− | <h3>core breakthroughs</h3> | + | <h3 style="padding: 0;margin-top: 0.5em;margin-bottom: 0.5em;">core breakthroughs</h3> |
<li>Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;</li> | <li>Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;</li> | ||
<li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li> | <li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li> | ||
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<div> | <div> | ||
<h2 id="Testing">CRISPeasy-out method (crOUT)</h2> | <h2 id="Testing">CRISPeasy-out method (crOUT)</h2> | ||
− | <h3>core breakthroughs</h3> | + | <h3 style="padding: 0;margin-top: 0.5em;margin-bottom: 0.5em;">core breakthroughs:</h3> |
<li>Characterization of the Cas9 device + optimized sgRNA (BBa_K2457006) in E. coli K12 MG1655 cells by tracking the impact on the cellular system (i.e., growth ratio);</li> | <li>Characterization of the Cas9 device + optimized sgRNA (BBa_K2457006) in E. coli K12 MG1655 cells by tracking the impact on the cellular system (i.e., growth ratio);</li> | ||
<li>Setting up a reliable data sheet of Cas9 activation machinery in a range of conditions to further expand its uses by SynBio and iGEM community;</li> | <li>Setting up a reliable data sheet of Cas9 activation machinery in a range of conditions to further expand its uses by SynBio and iGEM community;</li> |
Revision as of 04:18, 31 October 2017