Difference between revisions of "Team:ColumbiaNYC/HP/Silver"

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       <div class="row">
 
       <div class="row">
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          <h2>Listeria monocytogenes(hlyA gene)</h2>
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          <p>The hlyA gene codes for Listeriolysin O. When human cells have engulfed Listeria cells by phagocytosis, Listeriolysin
 +
            O becomes active and breaks open the phagosome, allowing the bacteria to live in the cytoplasm. Bacteria transformed
 +
            with Listeriolysin O are invasive microorganisms with the ability to hide from the immune system. With Listeriolysin,
 +
            bacteria can invade macrophages and then break open the phagosome, and the bacteria are free to live inside the
 +
            cytoplasm. Genetically engineered bacteria that express both listeriolysin and invasin are able to invade any
 +
            beta-1-integrin expressing cell type, and they can penetrate to the cytoplasm at high efficiencies.</p>
 +
          <br>
 +
          <p>
 +
            The hlyA gene (coding for Listerolysin O) will be part of the transkingdom RNAi plasmid (TRIP) that the team will use to
 +
            determine how well transkingdom RNA interference will work on different types of cancer cells and different oncogenes.
 +
            We aim to design the bacteria so that it only targets cancer cells.
 +
          </p>
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 +
        </div>
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        <div class="col-lg-12">
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          <h2>Yersinia enterocolitica(Inv gene)</h2>
 +
          <p>The inv gene codes for the invasin protein. The invasin protein on the surface of a Yersinia cell interacts with
 +
            beta-1-integrin receptors on the surface of eukaryotic cells. This triggers a signal transduction pathway, leading
 +
            to endocytosis of the whole bacterium. Bacteria transformed with invasin are invasive microorganisms with the
 +
            ability to hide from the immune system. Genetically engineered bacteria that express both listeriolysin and invasin
 +
            are able to invade any beta-1-integrin expressing cell type, and they can penetrate to the cytoplasm at high
 +
            efficiencies.
 +
          </p>
 +
          <br>
 +
          <p>
 +
            The inv gene (coding for invasin) will be part of the transkingdom RNAi plasmid (TRIP) that the team will use to determine
 +
            how well transkingdom RNA interference will work on different types of cancer cells and different oncogenes.
 +
            We aim to design the bacteria so that it only targets cancer cells.
 +
          </p>
 +
          <h2>Precautions taken with hlyA and Inv genes</h3>
 +
            <p>The host strain is auxotrophic nonpathegenic E. coli, the DapA- mutant, whose growth is inhibited in the absence
 +
              of lysine. We wore full personal protective equipment and worked only in specific areas designated only for
 +
              handling these specimen. We were under the supervision of researchers who are experienced and qualified in
 +
              working with BSL2 organisms.</p>
 +
        </div>
 +
  
 
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Revision as of 06:17, 31 October 2017

Safety - HP Silver

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Sint, explicabo dolores ipsam aliquam inventore corrupti.

Listeria monocytogenes(hlyA gene)

The hlyA gene codes for Listeriolysin O. When human cells have engulfed Listeria cells by phagocytosis, Listeriolysin O becomes active and breaks open the phagosome, allowing the bacteria to live in the cytoplasm. Bacteria transformed with Listeriolysin O are invasive microorganisms with the ability to hide from the immune system. With Listeriolysin, bacteria can invade macrophages and then break open the phagosome, and the bacteria are free to live inside the cytoplasm. Genetically engineered bacteria that express both listeriolysin and invasin are able to invade any beta-1-integrin expressing cell type, and they can penetrate to the cytoplasm at high efficiencies.


The hlyA gene (coding for Listerolysin O) will be part of the transkingdom RNAi plasmid (TRIP) that the team will use to determine how well transkingdom RNA interference will work on different types of cancer cells and different oncogenes. We aim to design the bacteria so that it only targets cancer cells.

Yersinia enterocolitica(Inv gene)

The inv gene codes for the invasin protein. The invasin protein on the surface of a Yersinia cell interacts with beta-1-integrin receptors on the surface of eukaryotic cells. This triggers a signal transduction pathway, leading to endocytosis of the whole bacterium. Bacteria transformed with invasin are invasive microorganisms with the ability to hide from the immune system. Genetically engineered bacteria that express both listeriolysin and invasin are able to invade any beta-1-integrin expressing cell type, and they can penetrate to the cytoplasm at high efficiencies.


The inv gene (coding for invasin) will be part of the transkingdom RNAi plasmid (TRIP) that the team will use to determine how well transkingdom RNA interference will work on different types of cancer cells and different oncogenes. We aim to design the bacteria so that it only targets cancer cells.

Precautions taken with hlyA and Inv genes

The host strain is auxotrophic nonpathegenic E. coli, the DapA- mutant, whose growth is inhibited in the absence of lysine. We wore full personal protective equipment and worked only in specific areas designated only for handling these specimen. We were under the supervision of researchers who are experienced and qualified in working with BSL2 organisms.

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This is where we do polymerase chain reaction

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This is where we do polymerase chain reaction

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This is where we do polymerase chain reaction