Difference between revisions of "Team:XJTLU-CHINA"

 
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         <title>XJTLU-CHINA 2017</title>
 
         <title>XJTLU-CHINA 2017</title>
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        <h1>Project description</h1>
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        <img id="homebanner" src="https://static.igem.org/mediawiki/2017/4/4a/Home_project.PNG" class="img-responsive center-block" alt="Responsive image">
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             <h2>1. Overview- Gastrointestinal Disease</h2>
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          <div>
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                <p>Brief introduction of the disease, how wide and how severe(e.g.death rate), usually not fatal, but there are rare cases existing, and our goal is to develop a quick and effective therapy to fight against one of the special cases.etc.</p>
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             <a href="#overview">
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            <p style="text-align:center;">
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            <h2>2. Special Case- S.aureus colonization</h2>
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                <h3><i>1) Description:</i></h3>
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                    <p>Apart from the enteritis caused by S.aureus toxin, there is another possible s.aureus infection, of which S.aureus would colonize in human intestine and form strong biofilms. The symptom can be severe: Fever, diarrhea, dehydration or even shock.</p>
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                <h3><i>2) Target Groups:</i></h3>
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                    <p>All population has the chance to get infected, but IBD patients seem to have higher risks due to their immunocompromising and the hospitalization environment.</p>
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<h1 id="overview" style="font-weight:900;text-align:center;margin-bottom:5%;">Overview</h1>
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                <h3><i>3) Current Situation of the Case:</i></h3>
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                    <p>Abuse of antibiotics which may result in MRSA development; Antibiotics may not be very efficient; Cost a lot and patients suffer a lot.etc.</p>
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      <h1 style="color:#4e7e94;"><i>S. aureus</i> Intestinal Colonization</h1>
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            <h2>3. Newly-discovered Anti-microbial Peptide</h2>
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      <p style="color:#4e7e94;">Human intestine is a highly complex microbial ecosystem. It accommodates commensal microflora species, various types of secretory fluids, fermentation metabolites and host defense molecules that promote colonization resistance to pathogenic bacteria. However, <i>Staphylococcus aureus</i>, one of the most opportunistic and invasive pathogens, is capable of disrupting this colonization resistance and infecting the intestine. Patients with <i>S. aureus</i> intestinal colonization suffer from fecal incontinence, diarrhea, pseudomembranous colitis and so on.</p>
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                <p>Brief introduction of amps, their advantages towards antibiotics, the mechanisms when killing pathogens. Introduction of DRGN1, by whom, where and when, which species, structure and features, effectiveness towards S.aureus, not yet been synthesized in bacteria.etc.</p>
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      <img class="img-responsive center-block comic2" src="https://static.igem.org/mediawiki/2017/8/8c/Home-1.png">
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            <h2>4. Probiotics</h2>
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                <p>Introduction of probiotics, the reason why using it as a host.etc.</p>
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            <h2>5. Delivery</h2>
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                <p>Enteric capsules or enteric micropelets, to avoid </p>
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      <h1>Antibiotic Overuse and Superbacteria</h1>
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        </div>
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      <p>Due to antibiotic overuse, <i>Staphylococcus aureus</i> develops resistance to various antibiotics and it becomes increasingly difficult to be eliminated. As a leading cause of hospital-acquired infections, methicillin-resistant <i>S. aureus</i> (MRSA) strains are multi-antibiotic resistant. Even the use of vancomycin — one of the last therapeutic resorts against <i>S. aureus</i>, becomes futile. Worse still, community-acquired MRSA strains are much more virulent and cause more morbidity and mortality than hospital-acquired MRSA.</p>
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            <h2>6. Our New-born Therapy</h2>
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                <p>Combination of 345, qs and anti-biofilm enzymes.etc.</p>
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      <img class="img-responsive center-block comic2" src="https://static.igem.org/mediawiki/2017/3/37/Home-2.png">
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            <h2>7. Future Application</h2>
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                <p>Together with anti-diarrheal medicine, produced in pharmaceutical industry
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                Together with intestinal nanobots, for detecting, medicine delivery and real-time effectiveness monitoring.etc.</p>
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      <h1 style="color:#f28884;">Antimicrobial Peptides — A Promising Alternative To Antibiotics</h1>
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      <p style="color:#f28884;">Antimicrobial peptides (AMPs) have a reputation for their efficient antimicrobial activity against bacteria, fungi and protozoa. Despite of some promising results in clinical trials, AMPs have difficulties to be made into drugs due to their poor metabolic stability in human body. Therefore, our project aims to genetically engineer <i>Lactococcus lactis</i> (Grenadier Guards) to closely detect <i>S. aureus</i> and throw AMPs (grenades) to eradicate them within a short time after infection.</p>
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            <h2>8. Reference</h2>
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      <h1>Further Applications</h1>
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      <p>Our genetically engineered <i>Lactococcus lactis</i> can be made into medicines, such as enteric coated tablets, together with anti-diarrheal medicines, to cure enteritis. In addition, as an important industrial microorganism, <i>L. lactis</i> can be used as food additives in fermented dairy products such as cheese, and yogurt. When they inhabit in the intestinal tract, not only do they regulate micro-ecological balance, but also prevent or reduce the chance of infection.</p>
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<h1 style="font-weight:bolder;color:#006934; ">Collaborators and Supporters</h1>
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<div class="container-fluid supporters-logos">
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        <a href="https://www.synbio-tech.com.cn"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/3/38/Synbio_tech_logo.png"></a>
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        <a href="https://www.wx2h.com/web/index.php"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/a/ab/Wuxi_No.2_people%27s_hospital.png"></a>
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    <div class="col-sm-4"> 
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        <a href="https://www.chinapeptides.qianyan.biz"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/e/e8/Qiang_yao_sheng_wu.png"></a>
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        <a href="https://www.neb.com"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/0/06/NEB_logo.png"></a>
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    </div>
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    <div class="col-sm-4">
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        <a href="https://www.snapgene.com"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/c/cb/Snapgene_logo.png"></a>
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    </div>
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    <div class="col-sm-4">
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        <a href="https://www.genscript.com"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/9/9b/Genscript.png"></a>
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              <h4>Location</h4>
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              <p  style="text-align:center;">Rm 363, Science Building<br>
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              Xi'an Jiaotong-Liverpool University<br>
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              111 Ren'ai Road, Suzhou, China<br>
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              215123
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              </p>
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          <div class="col-md-4 social">
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              <h4>Social</h4>
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              <a href=""><img src="https://static.igem.org/mediawiki/2017/9/9f/XJTLU_facebook.png" alt="facebook" width=30 height=30></a>
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              <a href=""><img src="https://static.igem.org/mediawiki/2017/7/72/XJTLU_blog.png" alt="blog" width=30 height=30></a>
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              <h4>Get in touch</h4>
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              <img src="https://static.igem.org/mediawiki/2017/1/19/XJTLU_email.png" alt="emali" width=30 height=30>
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              <p style="text-align:center;">igem@xjtlu.edu.cn</p>
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<div class="text-center" style="background:#003b73;">
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        <p style="text-align:center; color:white;">XJTLU-CHINA iGEM 2017</p>
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Latest revision as of 07:48, 31 October 2017

XJTLU-CHINA 2017
Responsive image

Overview

S. aureus Intestinal Colonization

Human intestine is a highly complex microbial ecosystem. It accommodates commensal microflora species, various types of secretory fluids, fermentation metabolites and host defense molecules that promote colonization resistance to pathogenic bacteria. However, Staphylococcus aureus, one of the most opportunistic and invasive pathogens, is capable of disrupting this colonization resistance and infecting the intestine. Patients with S. aureus intestinal colonization suffer from fecal incontinence, diarrhea, pseudomembranous colitis and so on.

Antibiotic Overuse and Superbacteria

Due to antibiotic overuse, Staphylococcus aureus develops resistance to various antibiotics and it becomes increasingly difficult to be eliminated. As a leading cause of hospital-acquired infections, methicillin-resistant S. aureus (MRSA) strains are multi-antibiotic resistant. Even the use of vancomycin — one of the last therapeutic resorts against S. aureus, becomes futile. Worse still, community-acquired MRSA strains are much more virulent and cause more morbidity and mortality than hospital-acquired MRSA.

Antimicrobial Peptides — A Promising Alternative To Antibiotics

Antimicrobial peptides (AMPs) have a reputation for their efficient antimicrobial activity against bacteria, fungi and protozoa. Despite of some promising results in clinical trials, AMPs have difficulties to be made into drugs due to their poor metabolic stability in human body. Therefore, our project aims to genetically engineer Lactococcus lactis (Grenadier Guards) to closely detect S. aureus and throw AMPs (grenades) to eradicate them within a short time after infection.

Further Applications

Our genetically engineered Lactococcus lactis can be made into medicines, such as enteric coated tablets, together with anti-diarrheal medicines, to cure enteritis. In addition, as an important industrial microorganism, L. lactis can be used as food additives in fermented dairy products such as cheese, and yogurt. When they inhabit in the intestinal tract, not only do they regulate micro-ecological balance, but also prevent or reduce the chance of infection.

Collaborators and Supporters

Location

Rm 363, Science Building
Xi'an Jiaotong-Liverpool University
111 Ren'ai Road, Suzhou, China
215123

Get in touch

emali

igem@xjtlu.edu.cn

XJTLU-CHINA iGEM 2017