Latest revision as of 07:48, 31 October 2017

XJTLU-CHINA 2017

Overview

S. aureus Intestinal Colonization

Human intestine is a highly complex microbial ecosystem. It accommodates commensal microflora species, various types of secretory fluids, fermentation metabolites and host defense molecules that promote colonization resistance to pathogenic bacteria. However, Staphylococcus aureus, one of the most opportunistic and invasive pathogens, is capable of disrupting this colonization resistance and infecting the intestine. Patients with S. aureus intestinal colonization suffer from fecal incontinence, diarrhea, pseudomembranous colitis and so on.

Antibiotic Overuse and Superbacteria

Due to antibiotic overuse, Staphylococcus aureus develops resistance to various antibiotics and it becomes increasingly difficult to be eliminated. As a leading cause of hospital-acquired infections, methicillin-resistant S. aureus (MRSA) strains are multi-antibiotic resistant. Even the use of vancomycin — one of the last therapeutic resorts against S. aureus, becomes futile. Worse still, community-acquired MRSA strains are much more virulent and cause more morbidity and mortality than hospital-acquired MRSA.

Antimicrobial Peptides — A Promising Alternative To Antibiotics

Antimicrobial peptides (AMPs) have a reputation for their efficient antimicrobial activity against bacteria, fungi and protozoa. Despite of some promising results in clinical trials, AMPs have difficulties to be made into drugs due to their poor metabolic stability in human body. Therefore, our project aims to genetically engineer Lactococcus lactis (Grenadier Guards) to closely detect S. aureus and throw AMPs (grenades) to eradicate them within a short time after infection.

Further Applications

Our genetically engineered Lactococcus lactis can be made into medicines, such as enteric coated tablets, together with anti-diarrheal medicines, to cure enteritis. In addition, as an important industrial microorganism, L. lactis can be used as food additives in fermented dairy products such as cheese, and yogurt. When they inhabit in the intestinal tract, not only do they regulate micro-ecological balance, but also prevent or reduce the chance of infection.

Collaborators and Supporters

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-/**************************************************************** Slider main ***************************************************************/
-jQuery(document).ready(function($){
-	var sliderContainers = $('.cd-slider-wrapper');
-	if( sliderContainers.length > 0 ) initBlockSlider(sliderContainers);
-	function initBlockSlider(sliderContainers) {
-		sliderContainers.each(function(){
-			var sliderContainer = $(this),
-				slides = sliderContainer.children('.cd-slider').children('li'),
-				sliderPagination = createSliderPagination(sliderContainer);
-			sliderPagination.on('click', function(event){
-				event.preventDefault();
-				var selected = $(this),
-					index = selected.index();
-				updateSlider(index, sliderPagination, slides);
-			});
-			sliderContainer.on('swipeleft', function(){
-				var bool = enableSwipe(sliderContainer),
-					visibleSlide = sliderContainer.find('.is-visible').last(),
-					visibleSlideIndex = visibleSlide.index();
-				if(!visibleSlide.is(':last-child') && bool) {updateSlider(visibleSlideIndex + 1, sliderPagination, slides);}
-			});
-			sliderContainer.on('swiperight', function(){
-				var bool = enableSwipe(sliderContainer),
-					visibleSlide = sliderContainer.find('.is-visible').last(),
-					visibleSlideIndex = visibleSlide.index();
-				if(!visibleSlide.is(':first-child') && bool) {updateSlider(visibleSlideIndex - 1, sliderPagination, slides);}
-			});
-		});
-	}
-	function createSliderPagination(container){
-		var wrapper = $('<ol class="cd-slider-navigation"></ol>');
-		container.children('.cd-slider').find('li').each(function(index){
-			var dotWrapper = (index == 0) ? $('<li class="selected"></li>') : $('<li></li>'),
-				dot = $('<a href="#0"></a>').appendTo(dotWrapper);
-			dotWrapper.appendTo(wrapper);
-			var dotText = ( index+1 < 10 ) ? '0'+ (index+1) : index+1;
-			dot.text(dotText);
-		});
-		wrapper.appendTo(container);
-		return wrapper.children('li');
-	}
-	function updateSlider(n, navigation, slides) {
-		navigation.removeClass('selected').eq(n).addClass('selected');
-		slides.eq(n).addClass('is-visible').removeClass('covered').prevAll('li').addClass('is-visible covered').end().nextAll('li').removeClass('is-visible covered');
-		//fixes a bug on Firefox with ul.cd-slider-navigation z-index
-		navigation.parent('ul').addClass('slider-animating').on('webkitTransitionEnd otransitionend oTransitionEnd msTransitionEnd transitionend', function(){
-			$(this).removeClass('slider-animating');
-		});
-	}
-	function enableSwipe(container) {
-		return ( container.parents('.touch').length > 0 );
-	}
-});
-        </script>
          <style>
-         /* --------------------------------
+         .comic{
+        padding-left:10%;
+        padding-right:10%;
+        padding-bottom:30px;
+        }
+        p {
+        font-size: 20px !important;
+        }
+        @media(min-width: 992px) {
+        .comic2 {
+        margin-top:30%;
+        }
+        }
-Primary style
+        </style>
+    </head>
+    <body>
+<div id="main-content" class="container-fluid">
+        <img id="homebanner" src="https://static.igem.org/mediawiki/2017/4/4a/Home_project.PNG" class="img-responsive center-block" alt="Responsive image">
+          <div>
+            <a href="#overview">
+             <p style="text-align:center;">
+             <img src="https://static.igem.org/mediawiki/2017/0/0d/Home_arrow_down.png" class="img-responsive center-block" alt="Responsive image" style="height: 80px; width: auto;">
+             </p>
+            </a>
+          </div>
+<h1 id="overview" style="font-weight:900;text-align:center;margin-bottom:5%;">Overview</h1>
--------------------------------- */
+   <div class="row comic" style="background:#d2e4ee;">
-*, *::after, *::before {
+      <div class="col-md-6">
-  box-sizing: border-box;
+      <h1 style="color:#4e7e94;"><i>S. aureus</i> Intestinal Colonization</h1>
-}
+      <p style="color:#4e7e94;">Human intestine is a highly complex microbial ecosystem. It accommodates commensal microflora species, various types of secretory fluids, fermentation metabolites and host defense molecules that promote colonization resistance to pathogenic bacteria. However, <i>Staphylococcus aureus</i>, one of the most opportunistic and invasive pathogens, is capable of disrupting this colonization resistance and infecting the intestine. Patients with <i>S. aureus</i> intestinal colonization suffer from fecal incontinence, diarrhea, pseudomembranous colitis and so on.</p>
+      </div>
+      <div class="col-md-6" >
+      <img class="img-responsive center-block comic2" src="https://static.igem.org/mediawiki/2017/8/8c/Home-1.png">
+      </div>
+   </div>
+   <div class="row comic">
+      <div class="col-md-6 col-md-push-6">
+      <h1>Antibiotic Overuse and Superbacteria</h1>
+      <p>Due to antibiotic overuse, <i>Staphylococcus aureus</i> develops resistance to various antibiotics and it becomes increasingly difficult to be eliminated. As a leading cause of hospital-acquired infections, methicillin-resistant <i>S. aureus</i> (MRSA) strains are multi-antibiotic resistant. Even the use of vancomycin — one of the last therapeutic resorts against <i>S. aureus</i>, becomes futile. Worse still, community-acquired MRSA strains are much more virulent and cause more morbidity and mortality than hospital-acquired MRSA.</p>
+      </div>
+      <div class="col-md-6 col-md-pull-6" >
+      <img class="img-responsive center-block comic2" src="https://static.igem.org/mediawiki/2017/3/37/Home-2.png">
+      </div>
+   </div>
+   <div class="row comic" style="background:#fbe1e0;">
+      <div class="col-md-6">
+      <h1 style="color:#f28884;">Antimicrobial Peptides — A Promising Alternative To Antibiotics</h1>
+      <p style="color:#f28884;">Antimicrobial peptides (AMPs) have a reputation for their efficient antimicrobial activity against bacteria, fungi and protozoa. Despite of some promising results in clinical trials, AMPs have difficulties to be made into drugs due to their poor metabolic stability in human body. Therefore, our project aims to genetically engineer <i>Lactococcus lactis</i> (Grenadier Guards) to closely detect <i>S. aureus</i> and throw AMPs (grenades) to eradicate them within a short time after infection.</p>
+      </div>
+      <div class="col-md-6" >
+      <img class="img-responsive center-block comic2" src="https://static.igem.org/mediawiki/2017/b/b5/Home-3.png">
+      </div>
+   </div>
+   <div class="row comic">
+      <div class="col-md-6 col-md-push-6">
+      <h1>Further Applications</h1>
+      <p>Our genetically engineered <i>Lactococcus lactis</i> can be made into medicines, such as enteric coated tablets, together with anti-diarrheal medicines, to cure enteritis. In addition, as an important industrial microorganism, <i>L. lactis</i> can be used as food additives in fermented dairy products such as cheese, and yogurt. When they inhabit in the intestinal tract, not only do they regulate micro-ecological balance, but also prevent or reduce the chance of infection.</p>
+      </div>
+      <div class="col-md-6 col-md-pull-6" >
+      <img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/5/55/Home-4.png">
+      </div>
+   </div>
+</div><!-- /.container -->
-html {
-  font-size: 62.5%;
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-body {
-  font-size: 1.6rem;
-  font-family: "Fira Sans", sans-serif;
-  color: #767070;
-  background-color: #ffffff;
-}
-a {
+<!-- banner -->
-  color: #ccd0c1;
+<div class="text-center" style="background:#cfe5d9;">
-   text-decoration: none;
+<h1 style="font-weight:bolder;color:#006934; ">Collaborators and Supporters</h1>
-}
+<div class="container-fluid supporters-logos">
+<div class="row">
+     <div class="col-sm-4">
+         <a href="https://www.synbio-tech.com.cn"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/3/38/Synbio_tech_logo.png"></a>
+     </div>
+     <div class="col-sm-4">
+         <a href="https://www.wx2h.com/web/index.php"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/a/ab/Wuxi_No.2_people%27s_hospital.png"></a>
+     </div>
+     <div class="col-sm-4">
+         <a href="https://www.chinapeptides.qianyan.biz"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/e/e8/Qiang_yao_sheng_wu.png"></a>
+     </div>
-/* --------------------------------
+</div>
+<div class="row">
-Slider
+    <div class="col-sm-4">
+         <a href="https://www.neb.com"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/0/06/NEB_logo.png"></a>
+     </div>
+     <div class="col-sm-4">
+         <a href="https://www.snapgene.com"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/c/cb/Snapgene_logo.png"></a>
+     </div>
+     <div class="col-sm-4">
+         <a href="https://www.genscript.com"><img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2017/9/9b/Genscript.png"></a>
+     </div>
+</div>
--------------------------------- */
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-    padding: 30px;
-  }
-}
-.cd-slider {
+<!-- footer -->
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+           <div class="col-md-4 loaction">
-.cd-slider li {
+               <h4>Location</h4>
-  position: absolute;
+               <p  style="text-align:center;">Rm 363, Science Building<br>
-  top: 0;
+               Xi'an Jiaotong-Liverpool University<br>
-  left: 0;
+Ren'ai Road, Suzhou, China<br>
-  height: 100%;
-  width: 100%;
+               </p>
-  -webkit-transform: translateX(100%);
+           </div>
-  -moz-transform: translateX(100%);
+           <div class="col-md-4 social">
-  -ms-transform: translateX(100%);
+               <h4>Social</h4>
-  -o-transform: translateX(100%);
+               <a href=""><img src="https://static.igem.org/mediawiki/2017/9/9f/XJTLU_facebook.png" alt="facebook" width=30 height=30></a>
-  transform: translateX(100%);
+               <a href=""><img src="https://static.igem.org/mediawiki/2017/7/72/XJTLU_blog.png" alt="blog" width=30 height=30></a>
-  -webkit-transition: -webkit-transform 0.6s;
+           </div>
-  -moz-transition: -moz-transform 0.6s;
+           <div class="col-md-4 contact">
-  transition: transform 0.6s;
+               <h4>Get in touch</h4>
-}
+               <img src="https://static.igem.org/mediawiki/2017/1/19/XJTLU_email.png" alt="emali" width=30 height=30>
-.cd-slider li.is-visible {
+               <p style="text-align:center;">igem@xjtlu.edu.cn</p>
-  -webkit-transform: translateX(0);
+           </div>
-  -moz-transform: translateX(0);
+        </div>
-  -ms-transform: translateX(0);
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-.cd-slider .cd-half-block {
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-.cd-slider .content {
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-.cd-slider .light-bg {
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-.cd-slider .btn {
-  display: inline-block;
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-  padding: 1em 1.6em;
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-  font-size: 1.3rem;
-  color: #ffffff;
-  border-radius: 50em;
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-  -moz-transition: background 0.3s;
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-.no-touch .cd-slider .btn:hover {
-  background: rgba(0, 0, 0, 0.6);
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-.cd-slider li:nth-of-type(2) .content {
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-.cd-slider li:nth-of-type(3) .content {
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-.cd-slider li:nth-of-type(4) .content {
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-.cd-slider h2 {
-  font-size: 2.2rem;
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-.cd-slider p {
-  font-size: 1.4rem;
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-.cd-slider h2, .cd-slider p {
-  line-height: 1.4;
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-@media only screen and (min-width: 900px) {
-  .cd-slider li {
-    pointer-events: none;
-    z-index: 1;
-    -webkit-transform: translateX(0);
-    -moz-transform: translateX(0);
-    -ms-transform: translateX(0);
-    -o-transform: translateX(0);
-    transform: translateX(0);
-    -webkit-transition: z-index 0s 0.6s;
-    -moz-transition: z-index 0s 0.6s;
-    transition: z-index 0s 0.6s;
-  }
-  .cd-slider li.is-visible {
-    pointer-events: auto;
-    z-index: 3;
-    -webkit-transition: z-index 0s 0s;
-    -moz-transition: z-index 0s 0s;
-    transition: z-index 0s 0s;
-  }
-  .cd-slider li.is-visible.covered {
-    /* list item still there, but covered by the list item entering the viewport (.is-visible) */
-    z-index: 2;
-  }
-  .cd-slider .cd-half-block {
-    height: 100%;
-    width: 50%;
-    float: right;
-  }
-  .cd-slider .cd-half-block.content {
-    -webkit-transform: translateX(200%);
-    -moz-transform: translateX(200%);
-    -ms-transform: translateX(200%);
-    -o-transform: translateX(200%);
-    transform: translateX(200%);
-    -webkit-transition: -webkit-transform 0.6s 0s ease-in-out;
-    -moz-transition: -moz-transform 0.6s 0s ease-in-out;
-    transition: transform 0.6s 0s ease-in-out;
-  }
-  .cd-slider .cd-half-block.image {
-    -webkit-transform: translateX(100%);
-    -moz-transform: translateX(100%);
-    -ms-transform: translateX(100%);
-    -o-transform: translateX(100%);
-    transform: translateX(100%);
-    -webkit-transition: -webkit-transform 0s 0.3s;
-    -moz-transition: -moz-transform 0s 0.3s;
-    transition: transform 0s 0.3s;
-  }
-  .cd-slider li.is-visible .cd-half-block.content,
-  .cd-slider li.is-visible .cd-half-block.image {
-    -webkit-transform: translateX(0%);
-    -moz-transform: translateX(0%);
-    -ms-transform: translateX(0%);
-    -o-transform: translateX(0%);
-    transform: translateX(0%);
-  }
-  .cd-slider li.is-visible .cd-half-block.content {
-    -webkit-transition: -webkit-transform 0.6s 0s ease-in-out;
-    -moz-transition: -moz-transform 0.6s 0s ease-in-out;
-    transition: transform 0.6s 0s ease-in-out;
-  }
-  .cd-slider .content {
-    /* vertically align its content */
-    display: table;
-    padding: 0 40px;
-  }
-  .cd-slider .content > div {
-    /* vertically align <div> inside div.content */
-    display: table-cell;
-    vertical-align: middle;
-  }
-  .cd-slider .btn {
-    padding: 1.4em 2em;
-    font-size: 1.4rem;
-  }
-  .cd-slider p {
-    font-size: 1.6rem;
-  }
-  .cd-slider h2 {
-    font-size: 3.5rem;
-    margin-bottom: 0;
-  }
-  .cd-slider h2, .cd-slider p {
-    line-height: 2;
-  }
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-@media only screen and (min-width: 1170px) {
-  .cd-slider .content {
-    padding: 0 90px;
-  }
-  .cd-slider h2 {
-    font-weight: 300;
-  }
-}
-/* --------------------------------
-Slider Navigation
--------------------------------- */
-.cd-slider-navigation {
-  /* you won't see this element in the html but it will be created using jQuery */
-  position: absolute;
-  z-index: 3;
-  left: 50%;
-  right: auto;
-  -webkit-transform: translateX(-50%);
-  -moz-transform: translateX(-50%);
-  -ms-transform: translateX(-50%);
-  -o-transform: translateX(-50%);
-  transform: translateX(-50%);
-  bottom: 30px;
-}
-.cd-slider-navigation li {
-  display: inline-block;
-  margin: 0 .25em;
-}
-.cd-slider-navigation li.selected a {
-  background-color: #ffffff;
-}
-.cd-slider-navigation a {
-  display: block;
-  height: 8px;
-  width: 8px;
-  border-radius: 50%;
-  color: transparent;
-  /* image replacement */
-  white-space: nowrap;
-  text-indent: 100%;
-  overflow: hidden;
-  border: 1px solid #ffffff;
-}
-@media only screen and (min-width: 900px) {
-  .cd-slider-navigation {
-    padding: 0.5em 1em;
-    background-color: rgba(0, 0, 0, 0.8);
-    /* fixes a bug on Firefox with ul.cd-slider-navigation z-index */
-    -webkit-transform: translateZ(2px) translateX(-50%);
-    -moz-transform: translateZ(2px) translateX(-50%);
-    -ms-transform: translateZ(2px) translateX(-50%);
-    -o-transform: translateZ(2px) translateX(-50%);
-    transform: translateZ(2px) translateX(-50%);
-    -webkit-transition: -webkit-transform 0.3s;
-    -moz-transition: -moz-transform 0.3s;
-    transition: transform 0.3s;
-  }
-  .cd-slider-navigation.slider-animating {
-    /* fixes a bug on Firefox with ul.cd-slider-navigation z-index */
-    -webkit-transform: translateX(-50%) scale(1);
-    -moz-transform: translateX(-50%) scale(1);
-    -ms-transform: translateX(-50%) scale(1);
-    -o-transform: translateX(-50%) scale(1);
-    transform: translateX(-50%) scale(1);
-  }
-  .cd-slider-navigation a {
-    height: 40px;
-    width: 40px;
-    line-height: 40px;
-    text-align: center;
-    /* reset style */
-    text-indent: 0;
-    border: none;
-    border-radius: 0;
-    color: #ffffff;
-    -webkit-font-smoothing: antialiased;
-    -moz-osx-font-smoothing: grayscale;
-  }
-  .cd-slider-navigation li.selected a {
-    background-color: transparent;
-    color: #f3b96c;
-  }
-}
-        </style>
-    </head>
-    <body>
-    <div id="main-content" class="container">
-    <img id="homebanner" src="https://static.igem.org/mediawiki/2017/4/4a/Home_project.PNG" style="width:100%;">
-    <div>
-    <a href="#Overview">
-    <p style="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/0/0d/Home_arrow_down.png" style="height: 80px; width: auto;"></p>
-    </a>
      </div>
-    <h1 id="Overview" style="text-align:center; font-family:'Helvetica',sans-serif; color: black;">Overview</h1>
+</div>
-    </div>
+<div class="text-center" style="background:#003b73;">
+        <p style="text-align:center; color:white;">XJTLU-CHINA iGEM 2017</p>
+</div>
-    <!-- Slider
+</footer>
-    ================================================== -->
-    <div class="cd-slider-wrapper">
-	<ul class="cd-slider">
-		<!-- First slide -->
-		<li class="is-visible">
-			<div class="cd-half-block image"></div>
-			<div class="cd-half-block content">
-				<div>
-				    <h2>S. aureus Intestinal Colonization</h2>
-					<p>Human intestine is a highly complex microbial ecosystem. It accommodates commensal microflora species, various types of secretory fluids, fermentation metabolites and host defense molecules that promote colonization resistance to pathogenic bacteria. However, Staphylococcus aureus, one of the most opportunistic and invasive pathogens, is capable of disrupting this colonization resistance and infecting the intestine. Patients with S. aureus intestinal colonization suffer from fecal incontinence, diarrhea, pseudomembranous colitis and so on.</p>
-                    <p><a class="btn btn-lg btn-primary" href="#" role="button">Learn more</a></p>
-				</div>
-			</div>
-		</li> <!-- .cd-half-block.content -->
-		<!-- Second slide -->
-		<li>
-			<div class="cd-half-block image"></div>
-			<div class="cd-half-block content light-bg">
-				<div>
-					<h2>Antibiotic Overuse and Superbacteria</h2>
-					<p>Due to antibiotic overuse, Staphylococcus aureus develops resistance to various antibiotics and it becomes increasingly difficult to be eliminated. As a leading cause of hospital-acquired infections, methicillin-resistant S. aureus (MRSA) strains are multi-antibiotic resistant. Even the use of vancomycin — one of the last therapeutic resorts against S. aureus, becomes futile. Worse still, community-acquired MRSA strains are much more virulent and cause more morbidity and mortality than hospital-acquired MRSA.</p>
-					<p><a class="btn btn-lg btn-primary" href="#" role="button">Learn more</a></p>
-				</div>
-			</div>
-		</li> <!-- .cd-half-block.content -->
-		<!-- Third slide -->
-		<li>
-			<div class="cd-half-block image"></div>
-			<div class="cd-half-block content light-bg">
-				<div>
-					<h2>Antimicrobial Peptides — A Promising Alternative To Antibiotics</h2>
-					<p>Antimicrobial peptides (AMPs) have a reputation for their efficient antimicrobial activity against bacteria, fungi and protozoa. Despite of some promising results in clinical trials, AMPs have difficulties to be made into drugs due to their poor metabolic stability in human body. Therefore, our project aims to genetically engineer Lactococcus lactis (Grenadier Guards) to closely detect S. aureus and throw AMPs (grenades) to eradicate them within a short time after infection.</p>
-					<p><a class="btn btn-lg btn-primary" href="#" role="button">Learn more</a></p>
-				</div>
-			</div>
-		</li> <!-- .cd-half-block.content -->
-		<!-- Fourth slide -->
-		<li>
-			<div class="cd-half-block image"></div>
-			<div class="cd-half-block content light-bg">
-				<div>
-					<h2>Further Applications</h2>
-					<p>Our genetically engineered Lactococcus lactis can be made into medicines, such as enteric coated tablets, together with anti-diarrheal medicines, to cure enteritis. In addition, as an important industrial microorganism, L. lactis can be used as food additives in fermented dairy products such as cheese, and yogurt. When they inhabit in the intestinal tract, not only do they regulate micro-ecological balance, but also prevent or reduce the chance of infection.</p>
-                    <p><a class="btn btn-lg btn-primary" href="#" role="button">Learn more</a></p>
-				</div>
-			</div>
-		</li> <!-- .cd-half-block.content -->
-	</ul> <!-- .cd-slider -->
-</div> <!-- .cd-slider-wrapper -->
      </body>
 </html>