Difference between revisions of "Team:XJTLU-CHINA/Protocols PCR purification"
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Latest revision as of 08:07, 31 October 2017
PCR purification using QIAquick PCR purification kit
- Add 500 μl Qiagen buffer PB
- Spin through a column twice, discard flow-through
- Wash 1x with 700 μl buffer PB
- Wash 2x with 760 μl buffer PE
- Discard liquid, spin dry at 17000g for 3 min
- Elute into a new tube twice with 50 μl of TE (100 μl total)
(Can be also referred to from iGEM protocol)
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