Difference between revisions of "Team:ASIJ TOKYO/Experiments"
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<h2 class="major">Competent Cell Check</h2> | <h2 class="major">Competent Cell Check</h2> | ||
| − | <p> | + | <p>The purpose of the competent cell check was to find the optimal concentration of the competent cells. We tested five different concentrations and found that 10pg/ul of the competent cells had the most efficient growth. </p> |
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<h2 class="major">Materials</h2> | <h2 class="major">Materials</h2> | ||
| − | <p> | + | <p>- Competent cell kit/ Transformation Efficiency Kit<br/>- 2.0m; microcentrifuge<br/>- Pipette: 1ul, 50ul, 200ul</br>- Incubator</br>- |
| + | Water bath</br>- SOC media</br>- Float rack</br>- Agar plates | ||
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 12:55, 31 October 2017
Competent Cell Check
The purpose of the competent cell check was to find the optimal concentration of the competent cells. We tested five different concentrations and found that 10pg/ul of the competent cells had the most efficient growth.
Materials
- Competent cell kit/ Transformation Efficiency Kit
- 2.0m; microcentrifuge
- Pipette: 1ul, 50ul, 200ul- Incubator-
Water bath- SOC media- Float rack- Agar plates
Method
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Gibson Assembly
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Materials
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Method
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Transformation
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Materials
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Method
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Electrophoresis
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Materials
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Method
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Restriction Digest
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Materials
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Method
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Bradford Protein Assay
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