Difference between revisions of "Team:UST Beijing/Experiments"

Revision as of 14:23, 31 October 2017

USTB-Beijing | Welcome

PanGu Cyclase Overview

Background

We used a composite part we constructed in 2016 iGEM competition (http://parts.igem.org/Part:BBa_K2072000:Design) for our following experiments. The plasmid pSB1C3/BBa_2072000 was transformed into E.coli BL21(DE3) cells and cultivated in 96-well microplates with following medium components: M9 salt mixture, chloramphenicol, glycerol and specific sugars, e.g. glucose, arabinose, or IPTG. PNPG was also included. The culture was shaken in 37 degrees C for up to 20 hours. The plate was analyzed with a microplate reader with wavelength at 405 and 620 nm. The data was collected in spreadsheet and analyzed using Matlab.

Strain used

Escherichia coli BL21(DE3)

Machines

CLC Sequence Viewer 6
Chimera 1.8
NCBI
Swiss Model

Methods

Protocols

Please click the link:
https://2017.igem.org/Competition/InterLab_Study>

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-Enspire 2300-001M<br/>
+CLC Sequence Viewer 6<br/>
-Sigma Laborzentrifugen<br/>
+Chimera 1.8<br/>
-Thermo Scientific Nanodrop 2000<br/>
+NCBI<br/>
-EVOS AMG microscope<br/>
+Swiss Model<br/>