Difference between revisions of "Team:UST Beijing/Basic Part"
TaoWenjing (Talk | contribs) |
TaoWenjing (Talk | contribs) |
||
| Line 177: | Line 177: | ||
</div> | </div> | ||
<br/><br/> | <br/><br/> | ||
| − | <object width="100%" height="500" data="https://static.igem.org/mediawiki/2017/ | + | <object width="100%" height="500" data="https://static.igem.org/mediawiki/2017/6/6f/T--UST_Beijing--xteam_hiuhiuhiu.pdf"> |
</object> | </object> | ||
</div> | </div> | ||
Revision as of 14:51, 31 October 2017
Wiki of InterLab by UST_Beijing
Background
We used a composite part we constructed in 2016 iGEM competition (http://parts.igem.org/Part:BBa_K2072000:Design) for our following experiments. The plasmid pSB1C3/BBa_2072000 was transformed into E.coli BL21(DE3) cells and cultivated in 96-well microplates with following medium components: M9 salt mixture, chloramphenicol, glycerol and specific sugars, e.g. glucose, arabinose, or IPTG. PNPG was also included. The culture was shaken in 37 degrees C for up to 20 hours. The plate was analyzed with a microplate reader with wavelength at 405 and 620 nm. The data was collected in spreadsheet and analyzed using Matlab.
Strain used
Escherichia coli BL21(DE3)
Machines
Enspire 2300-001M
Sigma Laborzentrifugen
Thermo Scientific Nanodrop 2000
EVOS AMG microscope
Methods
Protocols
Please click the link:
https://2017.igem.org/Competition/InterLab_Study>
