Difference between revisions of "Team:TU Darmstadt"

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{{TU_Darmstadt}}
 
 
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<h1> Welcome to iGEM 2017! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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        <title> iGEM TU Darmstadt </title>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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        <center><img src="https://static.igem.org/mediawiki/2017/d/dc/TUDarmstadtPDheader.png" width="50%"  ></center>
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        <h4>Chitosan is a biopolymer with both antibacterial and wound-healing properties. By linking fluorophores to chitosan oligomeres smart plasters can be produced, able to detect pathogenic bacteria via proteolytic activity. Therefore, production of designed chitosan for medical purpose is of special interest.  
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        </h4><br>
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            <img src="https://static.igem.org/mediawiki/2017/3/36/TUDarmstadtPDplaster.png" alt="chitosan hydrogel" width="100%">
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        <h3 id="red">Engineering <i>E.&nbsp;coli</i> for specific synthesis of designer chitosan
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<h5> Wiki template information </h5>
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            Our primary wet-lab goal is to engineer a synthetic biological circuit for the specific synthesis of chitosans in <i>E.&nbsp;coli</i>. The chemical properties as well as the bioactivity of chitosans mainly depend on three variables:  the length of the oligomers, their level of deacetylation and their patterns of deacetylation. 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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            Our enzymatic approach includes three enzymes. A chitin synthase (<i>Rhizobium&nbsp;leguminosarum bv.&nbsp;viciae</i>) catalyzes the oligomerization of N-acetylglucosamine-UDP monomers to chitin oligomers (tetramers and pentamers). 
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            Furthermore, two chitin deacetylases that differ in their regioselectivity (<i>Sinorhizobium&nbsp;meliloti</i>  (nodB) and <i>Puccina&nbsp;graminis f.&nbsp;sp.&nbsp;tritici</i>) are regulated orthogonally, making it possible to choose between two different types of deacetylation patterns.
  
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        </p>
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            <img src="https://static.igem.org/mediawiki/2017/2/2d/TUDarmstadtPDchitosansynthesis.png" alt="pathway chitosan" width="100%">
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        <h3 id="red">Application of chitosan oligomers:
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<h5> Editing your wiki </h5>
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            chitosan hydrogel for bacterial enzyme detection
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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            Downstream of the synthetic biological circuit for the synthesis of chitosan pentamers, we want to give an example for an explicit application of chitosan oligomers. This shall be accomplished by building a plaster for wounds carrying the chitosan hydrogel with chitosan oligomers linked to a fluorophore via a peptide chain. When the plaster is applied to a wound it can detect bacterial protease activities and thus diagnose wound infection. Proteases will cleave the peptide linker and release the fluorophore, that is then detectable via UV-light.
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            <img src="https://static.igem.org/mediawiki/2017/4/40/TUDarmstadtPDpeptidelinker.png" alt="peptide linker" width="100%">
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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UPLOAD FILES
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Revision as of 21:42, 29 June 2017

iGEM TU Darmstadt

Chitosan is a biopolymer with both antibacterial and wound-healing properties. By linking fluorophores to chitosan oligomeres smart plasters can be produced, able to detect pathogenic bacteria via proteolytic activity. Therefore, production of designed chitosan for medical purpose is of special interest.


chitosan hydrogel

Engineering E. coli for specific synthesis of designer chitosan

Our primary wet-lab goal is to engineer a synthetic biological circuit for the specific synthesis of chitosans in E. coli. The chemical properties as well as the bioactivity of chitosans mainly depend on three variables:  the length of the oligomers, their level of deacetylation and their patterns of deacetylation. 

Our enzymatic approach includes three enzymes. A chitin synthase (Rhizobium leguminosarum bv. viciae) catalyzes the oligomerization of N-acetylglucosamine-UDP monomers to chitin oligomers (tetramers and pentamers).  Furthermore, two chitin deacetylases that differ in their regioselectivity (Sinorhizobium meliloti (nodB) and Puccina graminis f. sp. tritici) are regulated orthogonally, making it possible to choose between two different types of deacetylation patterns.

pathway chitosan

Application of chitosan oligomers: chitosan hydrogel for bacterial enzyme detection

Downstream of the synthetic biological circuit for the synthesis of chitosan pentamers, we want to give an example for an explicit application of chitosan oligomers. This shall be accomplished by building a plaster for wounds carrying the chitosan hydrogel with chitosan oligomers linked to a fluorophore via a peptide chain. When the plaster is applied to a wound it can detect bacterial protease activities and thus diagnose wound infection. Proteases will cleave the peptide linker and release the fluorophore, that is then detectable via UV-light.

peptide linker