Difference between revisions of "Team:UST Beijing/InterLab"

Line 50: Line 50:
 
<body>
 
<body>
  
−
<!--Header_section-->
+
<!--Hero_Section-->
 +
<section id="hero_section" class="top_cont_outer">
 +
 
 +
<video autoplay loop style="width:100%; height:50%;">
 +
        <source src="https://static.igem.org/mediawiki/2017/5/59/UST_Beijing_MAIN_SCIEN.mp4">
 +
        </video>
 +
</section>
 +
 
 +
 
 
<header id="header_wrapper">
 
<header id="header_wrapper">
 
     <div class="header_box">
 
     <div class="header_box">
Line 61: Line 69:
 
<ul class="nav navbar-nav" id="mainNav">
 
<ul class="nav navbar-nav" id="mainNav">
  
−
  <li class="active">
+
  <li>
−
  <a id="ix0" href="https://2017.igem.org/Team:UST_Beijing/parts">
+
  <a href="https://2017.igem.org/Team:UST_Beijing/Background">
 
  <span id="ca-icon0"></span>
 
  <span id="ca-icon0"></span>
−
  <div class="scroll-link">Parts</div>
+
  <div class="scroll-link">Background</div></a></li>
−
  </a>
+
  <li><a id="cs2">
−
<ul>
+
−
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Basic_Part">basic_parts</a></li>
+
−
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Composite_Part">composite_parts</a></li>
+
−
                           
+
−
                        </ul>
+
−
  </li>
+
−
  <li><a href="https://2017.igem.org/Team:UST_Beijing/Project">
+
 
  <span id="ca-icon1"></span>
 
  <span id="ca-icon1"></span>
 
  <div class="scroll-link">Project</div></a>
 
  <div class="scroll-link">Project</div></a>
−
  <ul>
+
  <ul id="csy2">
−
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Overiew">Overiew</a></li>
+
                             <li><a href="https://2017.igem.org/Team:UST_Beijing/Pangu">Pangu</a></li>
−
                             <li><a href="https://2017.igem.org/Team:UST_Beijing/Experiments">Experiments</a></li>
+
                             <li><a href="https://2017.igem.org/Team:UST_Beijing/Experiments">Glucosidase</a></li>
−
                             <li><a href="https://2017.igem.org/Team:UST_Beijing/Results">Result</a></li>
+
 
                   <li><a href="https://2017.igem.org/Team:UST_Beijing/InterLab">InterLab</a></li>
 
                   <li><a href="https://2017.igem.org/Team:UST_Beijing/InterLab">InterLab</a></li>
 
                         </ul>
 
                         </ul>
 
  </li>
 
  </li>
−
  <li><a href="https://2017.igem.org/Team:UST_Beijing/Modeling">
+
  <li><a id="cs3" href="https://2017.igem.org/Team:UST_Beijing/Modeling">
 
  <span id="ca-icon2"></span>
 
  <span id="ca-icon2"></span>
−
  <div class="scroll-link">Modeling</div></a>
+
  <div class="scroll-link" >Modeling</div></a>
−
  <ul>
+
  <ul id="csy3">
−
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Modeling/Overiew">Overiew</a></li>
+
−
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Modeling/Doccking Protein">Doccking Protein</a></li>
+
−
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Modeling/Simmulation">Simmulation</a></li>
+
  
 
                         </ul></li>
 
                         </ul></li>
Line 94: Line 91:
 
  <span id="ca-icon3"></span>
 
  <span id="ca-icon3"></span>
 
  <div class="scroll-link">HomePage</div></a></li>
 
  <div class="scroll-link">HomePage</div></a></li>
−
  <li><a href="https://2017.igem.org/Team:UST_Beijing/Team.html">
+
  <li><a href="https://2017.igem.org/Team:UST_Beijing/Basic_Part">
 
  <span id="ca-icon4"></span>
 
  <span id="ca-icon4"></span>
−
  <div class="scroll-link">Team</div></a>
+
  <div class="scroll-link" >Parts</div></a></li>
−
  <ul>
+
  <li><a id="cs4">
−
              <li><a href="https://2017.igem.org/Team:UST_Beijing/Attributions">Attributions</a></li>
+
−
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Team">Members</a></li></ul></li>
+
−
  <li><a href="#Portfolio">
+
 
  <span id="ca-icon5"></span>
 
  <span id="ca-icon5"></span>
−
  <div class="scroll-link">Awards</div></a>
+
  <div class="scroll-link">Team</div></a>
−
<ul>
+
  <ul id="csy4">
−
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Modeling/HP/Silver">HP/Silver</a></li>
+
            <li><a href="https://2017.igem.org/Team:UST_Beijing/Attributions">Attributions</a></li>
−
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Collaborations">Collaborations</a></li>
+
                             <li><a href="https://2017.igem.org/Team:UST_Beijing/Team">Members</a></li></ul>
−
                             <li><a href="https://2017.igem.org/Team:UST_Beijing/Modeling/Simmulation">Simmulation</a></li>
+
−
 
+
−
                        </ul>
+
 
  </li>
 
  </li>
−
  <li><a href="https://2017.igem.org/Team:UST_Beijing/Note.html">
+
  <li><a id="cs5">
 
  <span id="ca-icon6"></span>
 
  <span id="ca-icon6"></span>
−
  <div class="scroll-link">Notebook</div></a>
+
  <div class="scroll-link">Awards</div></a>
−
  <ul>
+
<ul id="csy5">
−
              <li><a href="https://2017.igem.org/Team:UST_Beijing/Timeline">Timeline</a></li>
+
<li><a href="https://2017.igem.org/Team:UST_Beijing/HP/Silver">HP</a></li>
−
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Sheets">Sheets</a></li></ul></li>
+
<li><a href="https://2017.igem.org/Team:UST_Beijing/Collaborations">Collaborations</a></li>
 +
<li><a href="https://2017.igem.org/Team:UST_Beijing/Safety">Safety</a></li>
  
−
</ul>
+
                        </ul>
 +
</li>
 
       </div>
 
       </div>
 
</nav>
 
</nav>
 
     </div>
 
     </div>
 
</header>
 
</header>
−
<!--Header_section-->
+
 
 +
 
 +
 
 +
 
 +
<section id="aboutUs" style="background-image:url(https://static.igem.org/mediawiki/2017/f/f8/Ustbbjbj.png);background-position:center center;background-repeat:no-repeat;-moz-background-size: cover;background-size: cover;background-attachment:fixed;">
 +
<div class="inner_wrapper aboutUs-container fadeInLeft animated wow">
 +
 
  
  
Line 255: Line 254:
 
   </div>
 
   </div>
  
 +
</div>
 
</div>
 
</div>
  
−
<!-- footer -->
+
</section>
−
<footer class="text-center">
+
 
 +
<section class="page_section team" id="team" style="background-image:url(https://static.igem.org/mediawiki/2017/f/f8/Ustbbjbj.png);background-position:center center;background-repeat:no-repeat;-moz-background-size: cover;background-size: cover;background-attachment:fixed;">
 +
  <div class="container">
 +
    <h2 style="font-family: Arial">Sponsorship</h2>
 +
    <br />
 +
<div id="team" name="team">
 +
  <div class="container">
 +
    <div class="row centered">
 +
 
 +
      <div class="col-md-3 centered"> <img class="img img-circle lt-box" src="https://static.igem.org/mediawiki/2017/d/da/T--UST_Beijing--xteam01.jpg" height="120px" width="120px" alt="">
 +
        <div class="rt-box"><h4><strong>USTB</strong></h4>
 +
        <p style="text-align: center">University of Science & Technology Beijing</p><br/>
 +
</div>
 +
      </div>
 +
        <div class="col-md-3 centered"> <img class="img img-circle lt-box" src="https://static.igem.org/mediawiki/2017/3/30/USTB_huasheng_logo.png" height="120px" width="120px" alt="">
 +
        <div class="rt-box"><h4><strong>USTB-CBE</strong></h4>
 +
        <p style="text-align: center">University of Science & Technology Beijing<br />School of Chemistry and Biological Engineering</p><br/>
 +
</div>
 +
      </div>
 +
      <div class="col-md-3 centered"> <img class="img img-circle lt-box" src="https://static.igem.org/mediawiki/2017/9/95/T--UST_Beijing--xteam03.jpg" height="120px" width="120px" alt="">
 +
        <div class="rt-box"><h4><strong>BCU</strong></h4>
 +
        <p style="text-align: center">Beijing City University</p><br/>
 +
</div>
 +
      </div>
 +
      <div class="col-md-3 centered"> <img class="img img-circle lt-box" src="https://static.igem.org/mediawiki/2017/8/81/T--UST_Beijing--xteam02.jpg" height="120px" width="120px" alt="">
 +
        <div class="rt-box"><h4><strong>YUNNAN NOTOGINSENG</strong></h4>
 +
        <p style="text-align: center">An enterprise sponsor.</p><br/>
 +
</div>
 +
      </div>
 +
 
 +
    </div>
 +
  </div>
 +
</div>
 
     <div class="container" align="center">
 
     <div class="container" align="center">
 
         <p align="center" style="font-size: 5px"><br><h5>Copyright © 2017 UST_Beijing iGEM. All rights reserved.</h5></p>
 
         <p align="center" style="font-size: 5px"><br><h5>Copyright © 2017 UST_Beijing iGEM. All rights reserved.</h5></p>
 
     </div>
 
     </div>
−
</footer>
+
</div>
 +
</section>
 
</body>
 
</body>
  
−
 
−
<meta http-equiv="X-UA-Compatible" content="IE=edge">
 
−
<meta name="viewport" content="width=device-width, initial-scale=1.0">
 
 
<style>
 
<style>
−
#sideMenu
 
−
{display:none; /* Disable the display of the annoying side main menu*/}
 
−
#top_title
 
−
{display:none; /* Disable the annoying title*/}
 
−
#content
 
−
{padding:0px; width:90%; margin-left:5%; margin-right:5%;background-color: rgba(255,255,255,0);}
 
−
body
 
−
{margin: 0;
 
−
 
−
font-size: 24px;
 
−
color: #333333;
 
−
background-color: #fafafa;
 
−
background-image:url(https://static.igem.org/mediawiki/2017/9/9e/Ustb_kk.jpg);
 
−
background-position:center center;
 
−
background-repeat:no-repeat;
 
−
-moz-background-size: cover;
 
−
background-size: cover;
 
−
background-attachment:fixed;}
 
−
.container
 
−
{background-color: rgba(255,255,255,0.4);
 
−
padding-top:80px;}
 
−
p {font-size: 16px;}
 
−
h1,h2,h3,h4,h5,h6 {color: #282828;}
 
−
h1 {font-size:72px;} h2 {font-size:48px;} h3 {font-size:36px;}
 
−
h4 {font-size:32px;} h5 {font-size:28px;} h6 {font-size:24px;}
 
−
.panel-body{background-color:rgba(255, 255, 255, 0) !important;}
 
−
.panel-heading{background-color:rgba(255, 255, 255, 0.7)  !important;}
 
−
.panel-group{background-color:rgba(255, 255, 255, 0)  !important;}
 
−
.panel-transparent{background-color:rgba(255, 255, 255, 0) !important;}
 
−
.nav-pills>li.active>a{background-color:#93d66b !important;}
 
−
.nav-pills>li>a:hover{background-color:#b2dd75 !important; color:#FFFFFF!important;}
 
−
.nav-pills>li.active>a:hover{background-color:#93d66b !important;}
 
 
   ul ul {
 
   ul ul {
 
     background:#E4EAF1;
 
     background:#E4EAF1;
Line 307: Line 305:
 
     top:100%;
 
     top:100%;
 
     padding:0;
 
     padding:0;
−
display: none;
 
  
 
}
 
}
Line 316: Line 313:
  
  
 +
}
 +
body{
 +
font-family:Arial;
 +
}
 +
.scroll-link{
 +
font-size: 14px;
 
}
 
}
 
  ul ul li a {
 
  ul ul li a {
Line 321: Line 324:
  
 
}
 
}
−
  ul:hover ul {
+
 
−
    display: block;
+
−
}
+
 
  ul ul li a:hover {
 
  ul ul li a:hover {
 
     background:#FFFFFF;
 
     background:#FFFFFF;
  
 
}
 
}
−
ul ul ul {
 
−
    width:100%;
 
−
    position:absolute;
 
−
    left:100%;
 
−
    top:50%;
 
  
 +
ul ul ul {
 +
width: 100%;
 +
position: absolute;
 +
left: 100%;
 +
top: 50%;
 +
}
 
</style>
 
</style>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy1').hide(); //打开页面隐藏下拉列表
 +
$('#cs1').hover( //鼠标滑过导航栏目时
 +
function(){
 +
$('#csy1').show(); //显示下拉列表
 +
 +
},
 +
function(){
 +
$('#csy1').hide(); //鼠标移开后隐藏下拉列表
 +
}
 +
);
 +
$('#csy1').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
function(){
 +
$('#csy1').show();
 +
},
 +
function(){
 +
$('#csy1').hide();
 +
 +
}
 +
);
 +
$('cs1').hover( //鼠标滑过下拉列表是改变当前栏目样式
 +
);
 +
});
 +
</script>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy2').hide(); //打开页面隐藏下拉列表
 +
$('#cs2').hover( //鼠标滑过导航栏目时
 +
function(){
 +
$('#csy2').show(); //显示下拉列表
 +
 +
},
 +
function(){
 +
$('#csy2').hide(); //鼠标移开后隐藏下拉列表
 +
}
 +
);
 +
$('#csy2').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
function(){
 +
$('#csy2').show();
 +
},
 +
function(){
 +
$('#csy2').hide();
 +
 +
}
 +
);
 +
$('cs2').hover( //鼠标滑过下拉列表是改变当前栏目样式
 +
);
 +
});
 +
</script>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy3').hide(); //打开页面隐藏下拉列表
 +
$('#cs3').hover( //鼠标滑过导航栏目时
 +
function(){
 +
$('#csy3').show(); //显示下拉列表
 +
 +
},
 +
function(){
 +
$('#csy3').hide(); //鼠标移开后隐藏下拉列表
 +
}
 +
);
 +
$('#csy3').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
function(){
 +
$('#csy3').show();
 +
},
 +
function(){
 +
$('#csy3').hide();
 +
 +
}
 +
);
 +
$('cs3').hover( //鼠标滑过下拉列表是改变当前栏目样式
 +
);
 +
});
 +
</script>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy4').hide(); //打开页面隐藏下拉列表
 +
$('#cs4').hover( //鼠标滑过导航栏目时
 +
function(){
 +
$('#csy4').show(); //显示下拉列表
 +
 +
},
 +
function(){
 +
$('#csy4').hide(); //鼠标移开后隐藏下拉列表
 +
}
 +
);
 +
$('#csy4').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
function(){
 +
$('#csy4').show();
 +
},
 +
function(){
 +
$('#csy4').hide();
 +
 +
}
 +
);
 +
$('cs4').hover( //鼠标滑过下拉列表是改变当前栏目样式
 +
);
 +
});
 +
</script>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy5').hide(); //打开页面隐藏下拉列表
 +
$('#cs5').hover( //鼠标滑过导航栏目时
 +
function(){
 +
$('#csy5').show(); //显示下拉列表
 +
 +
},
 +
function(){
 +
$('#csy5').hide(); //鼠标移开后隐藏下拉列表
 +
}
 +
);
 +
$('#csy5').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
function(){
 +
$('#csy5').show();
 +
},
 +
function(){
 +
$('#csy5').hide();
 +
 +
}
 +
);
 +
$('cs5').hover( //鼠标滑过下拉列表是改变当前栏目样式
 +
);
 +
});
 +
</script>
 +
  
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/bootstrapmin?action=raw&amp;ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/bootstrapmin?action=raw&amp;ctype=text/javascript"></script>
Line 348: Line 476:
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/main?action=raw&amp;ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/main?action=raw&amp;ctype=text/javascript"></script>
  
−
</body>
 
−
<!--<link rel="stylesheet" href="css/danmuplayer.css">
 
−
<script src="js/danmuplayer.js"></script>
 
−
<script src="js/lib/jquery-1.11.1.min.js"></script>
 
−
<script>
 
−
  $("#danmup").DanmuPlayer({
 
−
   
 
−
    height: "480px", //区域的高度
 
−
    width: "800px" //区域的宽度
 
−
    ,urlToGetDanmu:"query.php"
 
−
    ,urlToPostDanmu:"stone.php"
 
−
  });
 
−
 
−
  $("#danmup .danmu-div").danmu("addDanmu",[
 
−
    { "text":"这是滚动弹幕" ,color:"white",size:1,position:0,time:2}
 
−
    ,{ "text":"我是帽子绿" ,color:"green",size:1,position:0,time:3}
 
−
    ,{ "text":"哈哈哈啊哈" ,color:"black",size:1,position:0,time:10}
 
−
    ,{ "text":"这是顶部弹幕" ,color:"yellow" ,size:1,position:1,time:3}
 
−
    ,{ "text":"这是底部弹幕" , color:"red" ,size:1,position:2,time:9}
 
−
    ,{ "text":"大家好，我是伊藤橙" ,color:"orange",size:1,position:1,time:3}
 
−
 
−
  ])
 
−
</script>-->
 
  
 
</html>
 
</html>

Revision as of 19:49, 31 October 2017

USTB-Beijing | Welcome

Wiki of InterLab by UST_Beijing


“All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader.

Materials & Methods

Plasmids used

Kit Plate 7
Positive Control (BBa_I20270): well 21B
Negative Control (BBa_R0040): well 21D
Test Device 1 (BBa_J364000): well 21F
Test Device 2 (BBa_J364001): well 21H
Test Device 3 (BBa_J364002): well 21J
Test Device 4 (BBa_J364003): well 21L
Test Device 5 (BBa_J364004): well 21N
Test Device 6 (BBa_J364005): well 21P

Strain used

Escherichia coli DH5α

Materials

FITC Standard: one tube with dried down FITC for creating a FITC standard
• LUDOX: one tube with 30% colloidal silica suspended in 1mL of water
• 1xPBS (phosphate buffered saline)
• LB (Luria Bertani) media
• Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH)
• 50 ml Falcon tube (or equivalent) or 250 ml shake flask for cell growth
• 1.5 ml eppendorf tubes for sample storage
• Ice bucket with ice
• Pipettes
• 96 well plate

Machines

Enspire 2300-001M
Sigma Laborzentrifugen
Thermo Scientific Nanodrop 2000
EVOS AMG microscope

Software

Microsoft Excel

Methods

Calibration
OD600 Reference point
FITC fluorescence standard curve
Test Device 1 (BBa_J364000): well 21F
Test Device 2 (BBa_J364001): well 21H
Test Device 3 (BBa_J364002): well 21J
Test Device 4 (BBa_J364003): well 21L
Test Device 5 (BBa_J364004): well 21N
Test Device 6 (BBa_J364005): well 21P

Cell measurement

Transformation
Measurements

Protocols

Please click the link:
https://2017.igem.org/Competition/InterLab_Study>






Sponsorship


USTB

University of Science & Technology Beijing


USTB-CBE

University of Science & Technology Beijing
School of Chemistry and Biological Engineering


BCU

Beijing City University


YUNNAN NOTOGINSENG

An enterprise sponsor.



Copyright © 2017 UST_Beijing iGEM. All rights reserved.