Difference between revisions of "Team:Amazonas Brazil/Demonstrate"

Line 109: Line 109:
 
                 </div>
 
                 </div>
  
−
                 <div style='position:absolute;top:10%; left:48%;'>                   
+
                 <div style='position:absolute;top:5%; left:40%;'>                   
 
                 <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto; width:45%; height:45%;"
 
                 <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto; width:45%; height:45%;"
 
                 src="https://static.igem.org/mediawiki/2017/0/02/Amazonas_Brazil_2017Demonstrate1.png"/>                   
 
                 src="https://static.igem.org/mediawiki/2017/0/02/Amazonas_Brazil_2017Demonstrate1.png"/>                   

Revision as of 00:31, 1 November 2017

Wiki_iGEM_Amazonas

DEMONSTRATE

Demonstrate

Erro
Erro
Erro

Toolbox modules assembly

How we constructed our circuits

core breakthroughs

  • Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;
  • Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;

    • CRISPeasy-out method (crOUT)

    core breakthroughs:

  • Characterization of the Cas9 device + optimized sgRNA (BBa_K2457006) in E. coli K12 MG1655 cells by tracking the impact on the cellular system (i.e., growth ratio);
  • Setting up a reliable data sheet of Cas9 activation machinery in a range of conditions to further expand its uses by SynBio and iGEM community;

    • Teste