Difference between revisions of "Team:Amazonas Brazil/Demonstrate"

Line 128: Line 128:
 
                
 
                
  
−
               <div>
+
               <div style="position: absolute;">
 
                 <h1 style=" margin: 0;padding-top: 0.1em;"id="Assembly"></h1>
 
                 <h1 style=" margin: 0;padding-top: 0.1em;"id="Assembly"></h1>
 
                 <h2 style="margin-top: 1.4em;">Toolbox modules assembly</h2>
 
                 <h2 style="margin-top: 1.4em;">Toolbox modules assembly</h2>
Line 136: Line 136:
 
                 <li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li>
 
                 <li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li>
 
               </div>
 
               </div>
−
               <div>
+
               <div style="position: absolute;">
 
               <h1 style=" margin: 0;padding-top: 0.1em;"id="Testing"></h1>
 
               <h1 style=" margin: 0;padding-top: 0.1em;"id="Testing"></h1>
 
                 <h2 style="margin-top: 1.4em;">CRISPeasy-out method (crOUT)</h2>
 
                 <h2 style="margin-top: 1.4em;">CRISPeasy-out method (crOUT)</h2>

Revision as of 02:11, 1 November 2017

Wiki_iGEM_Amazonas

DEMONSTRATE

Demonstrate

Erro

Toolbox modules assembly

How we constructed our circuits

core breakthroughs

  • Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;
  • Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;

    • CRISPeasy-out method (crOUT)

    core breakthroughs:

  • Characterization of the Cas9 device + optimized sgRNA (BBa_K2457006) in E. coli K12 MG1655 cells by tracking the impact on the cellular system (i.e., growth ratio);
  • Setting up a reliable data sheet of Cas9 activation machinery in a range of conditions to further expand its uses by SynBio and iGEM community;

    • Teste