Difference between revisions of "Team:Amazonas Brazil/Project"

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         <li><a class="Link-pg" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png"  width="104.04";height="90"; class="Menu_image"></a></li>
 
         <li><a class="Link-pg" href="https://2017.igem.org/Team:Amazonas_Brazil"><img src="https://static.igem.org/mediawiki/2017/d/d4/T--Amazonas_Brazil--LogoBranco.png"  width="104.04";height="90"; class="Menu_image"></a></li>
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         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Project">Description</a></li>
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         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate">Demonstrate</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Model">Modelling</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/Model">Modelling</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/InterLab">Interlab</a></li>
 
         <li><a class="bar_text" href="https://2017.igem.org/Team:Amazonas_Brazil/InterLab">Interlab</a></li>
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  <div class="hero-full-container background-image-container white-text-container" style="background: linear-gradient(to left, #263D9E, #3F2759);">
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<div class="hero-full-container background-image-container white-text-container" style="background-image: url(https://static.igem.org/mediawiki/2017/1/11/T--Amazonas_Brazil--PROJECT.jpg); " >
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    <div class="container">
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  <div class="container">
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            <div class="text-content  text-center">
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          <div class="text-content  text-center">
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              <h2 class="Page_Title" align="middle">DEMONSTRATE</h2>
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            <h2 class="Page_Title" align="middle">PROJECT</h2>
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  </div>
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          <h2 class="Page_Subtitle" align="middle">pCRISPeasy</h2>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
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           <div class="col-xs-12";>
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             <h1 class="First"style="text-align: center;">Demonstrate</h1>
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             <h1 class="First"style="text-align: center;height: 2.3em;">Navigate into CRISPeasy roadmap</h1>
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              <div >
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            <div>
  
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                <div style='position:relative;top:0px; left:0px'>
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              <div style='position:relative;top:0px; left:0px;'> <!-- div para toda a imagem central -->
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      <!--     <div style="position: absolute;top:30% ;left:5% ">  -->
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                <img style="display: block; margin-left: auto; margin-right: auto;width: 26%;height: 20%"
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                src="https://static.igem.org/mediawiki/2017/0/02/Amazonas_Brazil_2017Demonstrate1.png">
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      <!--      </div> -->  
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                <div style='position:absolute;top:100%; left:6%;'>                 
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              <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
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                <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto; width:45%; height:45%;"
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                          src="https://static.igem.org/mediawiki/2017/a/ab/Fluxo.png"/>
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                src="https://static.igem.org/mediawiki/2017/8/87/Amazonas_Brazil_2017OPERATION1.png"/>                 
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                </div>
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                <!--
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                <div style='position:absolute;top:47%; left:45%;'> <!-- div para imagens que se sobrepoem -->
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                <div style='position:absolute;top:53%; left:4%;'>                
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                  <a href="https://2017.igem.org/Team:Amazonas_Brazil/Human_Practices">
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                <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto; width:45%; height:45%;"
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                      <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto; width:150px; height:50px;"
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                src="https://static.igem.org/mediawiki/2017/0/0d/Amazonas_Brazil_2017CROUT1.png"/>                
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                                              src="https://static.igem.org/mediawiki/2017/1/1a/1_HumanPratices.png"/>
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                  </a>
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                <div style='position:absolute;top:53%; left:48%;'>                
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                  <div style='position:absolute;top:13%; left:45%;'> <!-- div para imagens que se sobrepoem -->
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                <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto; width:45%; height:45%;"
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                  <a href="#Specifications">
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                src="https://static.igem.org/mediawiki/2017/1/12/Amazonas_Brazil_2017CRIN1.png"/>                
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                      <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
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                </div>
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                                              src="https://static.igem.org/mediawiki/2017/3/37/2_Specifications.png"/>
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                  </a>
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                <div style='position:absolute;top:26%; left:67%;'> <!-- div para imagens que se sobrepoem -->
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                src="https://static.igem.org/mediawiki/2017/0/02/Amazonas_Brazil_2017Demonstrate1.png"/>                   
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                  <a href="#Design">
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                </div>
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                      <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
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                -->
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                  </a>
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                <div style='position:absolute;top:66%; left:67%;'> <!-- div para imagens que se sobrepoem -->
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                  <a href="https://2017.igem.org/Team:Amazonas_Brazil/Model">
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                      <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
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                                              src="https://static.igem.org/mediawiki/2017/7/79/4_Modelling.png"/>
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                  </a>
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                </div>
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                <div style='position:absolute;top:77%; left:45%;'> <!-- div para imagens que se sobrepoem -->
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                  <a href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate#Assembly">
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                      <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
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                                              src="https://static.igem.org/mediawiki/2017/4/4c/5_Assembly.png"/>
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                  </a>
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                <div style='position:absolute;top:66%; left:25%;'> <!-- div para imagens que se sobrepoem -->
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                  <a href="https://2017.igem.org/Team:Amazonas_Brazil/Demonstrate#Testing">
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                      <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
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                                              src="https://static.igem.org/mediawiki/2017/a/a4/6_Testing.png"/>
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                  </a>
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                </div>
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                <div style='position:absolute;top:27%; left:22%;'> <!-- div para imagens que se sobrepoem -->
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                  <a href="https://2017.igem.org/Team:Amazonas_Brazil/Applied_Design">
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                      <img alt="Erro" style="display: block; margin-left: auto; margin-right: auto"
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                                              src="https://static.igem.org/mediawiki/2017/3/38/7_Implementation.png"/>
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                  </a>
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               </div>
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            </div>
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            <div>
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              <div>
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              <h1 style=" margin: 0;padding-top: 0.1em;"id="Specifications"></h1>
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                <h1 style=" margin: 0;padding-top: 0.1em;"id="Assembly"></h1>
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              <h2 style="margin-top: 1.4em;">Specifications</h2>
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                <h2 style="margin-top: 1.4em;">Toolbox modules assembly</h2>
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              <h3>Our proposal is to provide a bacterial genome editing approach based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR</h3>
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                <h4 style="padding: 0;">How we constructed our circuits</h4>
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              <p class="p">
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                <h3 style="padding: 0;margin-top: 0.5em;margin-bottom: 0.5em;">core breakthroughs</h3>
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                 Scientific community had achieved great results using CRISPR-Cas9 mediated genome editing techniques.
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                 <li>Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;</li>
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                 While these methods are moving forward, some basic methodological aspects related to this approach could
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                 <li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li>
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                be further improved to expand this science revolution.
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              </div>
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                </p>
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              <div>
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            </div>
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              <h1 style=" margin: 0;padding-top: 0.1em;"id="Testing"></h1>
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            <div>
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                <h2 style="margin-top: 1.4em;">CRISPeasy-out method (crOUT)</h2>
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            <h1 style=" margin: 0;padding-top: 0.1em;" id="Design" ></h1>
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                <h3 style="padding: 0;margin-top: 0.5em;margin-bottom: 0.5em;">core breakthroughs:</h3>
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            <h2 style="margin-top: 1.4em;">Design</h2>
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                <li>Characterization of the Cas9 device + optimized sgRNA (BBa_K2457006) in E. coli K12 MG1655 cells by tracking the impact on the cellular system (i.e., growth ratio);</li>
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            <h3>Check all the parts involved and our approaches with closer details!</h3>
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                <li>Setting up a reliable data sheet of Cas9 activation machinery in a range of conditions to further expand its uses by SynBio and iGEM community;</li>
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              <p class="p">
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              </div>
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                Cas9 (BBa_K2457001): Standardized Cas9 device, regulated by the AraC_pBAD regulatory module.
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                In the single guide RNA presence, it forms a catalytically active ribonucleoprotein (RNP) complex
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               <div style="background-color:#323232"> <!-- Div para imagens-->
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                which cleaves specifics target sequences from DNA, leading to double-strand breaks. Through cell repair
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                <div>
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                pathways, it will lays the road to genomic editing in living cells.
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                  <p>
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              </p>
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                    Teste
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            </div>
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                  </p>
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            <div>
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                </div>
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               <img src="https://static.igem.org/mediawiki/2017/5/5f/T--Amazonas_Brazil--infografico_project.png" style="background-size: cover;  background-repeat: no-repeat;width:80%; margin-left: 10%;">
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Revision as of 02:46, 1 November 2017

Wiki_iGEM_Amazonas

PROJECT

pCRISPeasy

Navigate into CRISPeasy roadmap

Specifications

Our proposal is to provide a bacterial genome editing approach based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR

Scientific community had achieved great results using CRISPR-Cas9 mediated genome editing techniques. While these methods are moving forward, some basic methodological aspects related to this approach could be further improved to expand this science revolution.

Design

Check all the parts involved and our approaches with closer details!

Cas9 (BBa_K2457001): Standardized Cas9 device, regulated by the AraC_pBAD regulatory module. In the single guide RNA presence, it forms a catalytically active ribonucleoprotein (RNP) complex which cleaves specifics target sequences from DNA, leading to double-strand breaks. Through cell repair pathways, it will lays the road to genomic editing in living cells.