Mctastolfi (Talk | contribs) |
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<div> | <div> | ||
<h1 style=" margin: 0;padding-top: 0.1em;"id="Assembly"></h1> | <h1 style=" margin: 0;padding-top: 0.1em;"id="Assembly"></h1> | ||
− | <h2 | + | <h2 >Toolbox modules assembly</h2> |
<h4 style="padding: 0;">How we constructed our circuits</h4> | <h4 style="padding: 0;">How we constructed our circuits</h4> | ||
− | <h3 style="padding: 0 | + | <h3 style="padding: 0;margin-bottom: 0.5em;">core breakthroughs</h3> |
<li>Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;</li> | <li>Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;</li> | ||
<li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li> | <li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li> |
Revision as of 04:49, 1 November 2017
Toolbox modules assembly
How we constructed our circuits
core breakthroughs
CRISPeasy-out method (crOUT)
core breakthroughs:
Teste