Difference between revisions of "Team:Amazonas Brazil/Demonstrate"

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               <div>
 
               <div>
 
                 <h1 style=" margin: 0;padding-top: 0.1em;"id="Assembly"></h1>
 
                 <h1 style=" margin: 0;padding-top: 0.1em;"id="Assembly"></h1>
                 <h2 style="margin-top: 1.4em;">Toolbox modules assembly</h2>
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                 <h2 >Toolbox modules assembly</h2>
 
                 <h4 style="padding: 0;">How we constructed our circuits</h4>
 
                 <h4 style="padding: 0;">How we constructed our circuits</h4>
                 <h3 style="padding: 0;margin-top: 0.5em;margin-bottom: 0.5em;">core breakthroughs</h3>
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                 <h3 style="padding: 0;margin-bottom: 0.5em;">core breakthroughs</h3>
 
                 <li>Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;</li>
 
                 <li>Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;</li>
 
                 <li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li>
 
                 <li>Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;</li>

Revision as of 04:49, 1 November 2017

Wiki_iGEM_Amazonas

DEMONSTRATE

Toolbox modules assembly

How we constructed our circuits

core breakthroughs

  • Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;
  • Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;
  • CRISPeasy-out method (crOUT)

    core breakthroughs:

  • Characterization of the Cas9 device + optimized sgRNA (BBa_K2457006) in E. coli K12 MG1655 cells by tracking the impact on the cellular system (i.e., growth ratio);
  • Setting up a reliable data sheet of Cas9 activation machinery in a range of conditions to further expand its uses by SynBio and iGEM community;
  • Teste