Skywalkerest (Talk | contribs) |
Skywalkerest (Talk | contribs) |
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− | <h2>1. Transform <i>E.coli</i> DH5α with these following plasmids:</h2> | + | <h2>1. Transform <i>E. coli</i> DH5α with these following plasmids:</h2> |
<h3> a) Positive Control</h3> | <h3> a) Positive Control</h3> | ||
<h3> b) Negative Control</h3> | <h3> b) Negative Control</h3> | ||
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<h3> h) Test Device 6</h3> | <h3> h) Test Device 6</h3> | ||
− | <h2>2. Pick 2 colonies from each | + | <h2>2. Pick 2 colonies from each plate and inoculate it on 5 mL LB medium + Chloramphenicol. Grow the cells overnight (16 hours) at 37°C and 220 rpm.</h2> |
<h2>3. Cell growth, sampling, and assay</h2> | <h2>3. Cell growth, sampling, and assay</h2> | ||
<h3> a) Set your instrument to read OD600 (as OD calibration setting)</h3> | <h3> a) Set your instrument to read OD600 (as OD calibration setting)</h3> |
Latest revision as of 07:50, 1 November 2017