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<h3 style="color: #00a98f;">Introduction:</h3> | <h3 style="color: #00a98f;">Introduction:</h3> | ||
<p>In order to quantify the peptide concentration accumulation with <i>S. aureus</i> proliferation under different conditions, we regarded the each <i>S. aureus</i> as isolated unit. This prediction will be incorporated into the equation to calculate(simulate) the total protein amount at the community level.</p> | <p>In order to quantify the peptide concentration accumulation with <i>S. aureus</i> proliferation under different conditions, we regarded the each <i>S. aureus</i> as isolated unit. This prediction will be incorporated into the equation to calculate(simulate) the total protein amount at the community level.</p> | ||
− | <img src="https://static.igem.org/mediawiki/2017/7/72/T--TMMU-China--model1.png"> | + | <img width="55%" src="https://static.igem.org/mediawiki/2017/7/72/T--TMMU-China--model1.png"> |
<p style="text-align: center;font-family:'Open Sans', sans-serif;font-size: 13px;">Figure 1. The agr-Quorum Sensing Sender System of S.aureus</p> | <p style="text-align: center;font-family:'Open Sans', sans-serif;font-size: 13px;">Figure 1. The agr-Quorum Sensing Sender System of S.aureus</p> | ||
<p>The Agr-QS system is a quorum-sensing system in <i>S. aureus</i> and typical for Gram-positive bacteria. As shown in figure 1, when the transcription factor from AgrA phosphorylation binds with P32 promoter, agrB and agrD were continuously promoted to express AgrB and AgrD respectively[2]. In the Agr system, the protein of interest production process is briefly shown as below:</p> | <p>The Agr-QS system is a quorum-sensing system in <i>S. aureus</i> and typical for Gram-positive bacteria. As shown in figure 1, when the transcription factor from AgrA phosphorylation binds with P32 promoter, agrB and agrD were continuously promoted to express AgrB and AgrD respectively[2]. In the Agr system, the protein of interest production process is briefly shown as below:</p> | ||
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<h3 style="color: #00a98f;">Introduction:</h3> | <h3 style="color: #00a98f;">Introduction:</h3> | ||
<p>In order to predict the gene expression pattern of the four types of Agr system under different concentration of AIP, we regarded the each <i>S. aureus</i> as isolated unit. We would like to have the receiver part of the Agr-QS system in form of a biobrick. It becomes useful to think about inputs and outputs to and from such a biobrick. We shall use the concentration of AIP as a universal signal to "connect" biobricks to each other. The function of AIP to receiver was shown as following in detail.</p> | <p>In order to predict the gene expression pattern of the four types of Agr system under different concentration of AIP, we regarded the each <i>S. aureus</i> as isolated unit. We would like to have the receiver part of the Agr-QS system in form of a biobrick. It becomes useful to think about inputs and outputs to and from such a biobrick. We shall use the concentration of AIP as a universal signal to "connect" biobricks to each other. The function of AIP to receiver was shown as following in detail.</p> | ||
− | <img width=" | + | <img width="55%" src="https://static.igem.org/mediawiki/2017/f/fc/T--TMMU-China--model7.png"> |
<p style="text-align: center;font-family:'Open Sans', sans-serif;font-size: 13px;">Figure 3. the gene expression model of agr system.</p> | <p style="text-align: center;font-family:'Open Sans', sans-serif;font-size: 13px;">Figure 3. the gene expression model of agr system.</p> | ||
Latest revision as of 09:35, 1 November 2017
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