Construct Model
Building off of a rapamycin induced split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream products, c-Myc and COX-2 (Peking iGEM Team 2015, 2015). These products are assembled into a construct composing of fusion proteins as well as split luciferase fragments (COX-2 - nLuc - FRB and FKBP - cLuc - c-Myc). In the presence of rapamycin, the interacting protein partners dimerize and subsequently cause luciferase to activate. In order to test this model, we created vectors of our constructs and inserted them into e-coli cells.