Difference between revisions of "Team:CIEI-China"

 
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<div id="my-container">
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<h1> E. Coli </h1>
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<section class="banner" role="banner">
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<p>Your team has been approved and you are ready to start the iGEM season!</p>
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                    <h1 class="animated slideInLeft">Terminator of food waste</h1>
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                    <p class="animated slideInRight"> </p>
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<h5>Before you start: </h5>
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                    <a href="#a1" class="btn btn-large"><i class="fa fa-angle-double-down" aria-hidden="true"></i>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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UPLOAD FILES
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        <div class="first-level" id="a1">
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            Terminator of food waste
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        <p class="my-content">
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            Disposal of food waste is a huge concern in the society. Improper disposal brings serious pollution and causes tremendous nutrition loss. Considering the low osmotic pressure which is caused by high concentration of salt and sugar in food waste, survivability of the microbes and their efficiency during decomposition of the waste are significantly affected by this hypertonic environment. Our team aims to improve the survivability of the microbes and their decomposition efficiency in lower osmotic pressure conditions. Thus, we planned to transfer salt resistant genes into the functional microbes. In this research, we modified the yeast, specifically saccharomycetes cerevisiae, by inserting the <i>E. coli</i> glutamate synthase (<i>gltB</i>) gene and trehalose synthesis related genes (<i>ScTPS1</i> and <i>SpTPS1</i>) into it, to make the yeast not only can decompose the food waste efficiently but is able to resist low osmotic pressure. These genes help produce small molecular substances (glutamate and trehalose), which enable the cells to automatically adjust the osmotic pressure in high concentration environments. Based on the survivability test of yeast under different salt concentrations which was set according to our salinity test results of the collected food waste, we assured the effectiveness of our method in helping yeast tolerate low osmotic pressure environments and improving their decomposition efficiency. In this way, we can better take care of the troublesome food waste in a more cost-effective, energy saving, and environmentally friendly way.
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Latest revision as of 13:07, 1 November 2017

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Terminator of food waste

Disposal of food waste is a huge concern in the society. Improper disposal brings serious pollution and causes tremendous nutrition loss. Considering the low osmotic pressure which is caused by high concentration of salt and sugar in food waste, survivability of the microbes and their efficiency during decomposition of the waste are significantly affected by this hypertonic environment. Our team aims to improve the survivability of the microbes and their decomposition efficiency in lower osmotic pressure conditions. Thus, we planned to transfer salt resistant genes into the functional microbes. In this research, we modified the yeast, specifically saccharomycetes cerevisiae, by inserting the E. coli glutamate synthase (gltB) gene and trehalose synthesis related genes (ScTPS1 and SpTPS1) into it, to make the yeast not only can decompose the food waste efficiently but is able to resist low osmotic pressure. These genes help produce small molecular substances (glutamate and trehalose), which enable the cells to automatically adjust the osmotic pressure in high concentration environments. Based on the survivability test of yeast under different salt concentrations which was set according to our salinity test results of the collected food waste, we assured the effectiveness of our method in helping yeast tolerate low osmotic pressure environments and improving their decomposition efficiency. In this way, we can better take care of the troublesome food waste in a more cost-effective, energy saving, and environmentally friendly way.