Difference between revisions of "Team:CIEI-BJ/Safety"

 
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<div id="my-container">
 
<div id="my-container">
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<div id="my-sidebar">
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<div class="side-top">
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<img src="https://static.igem.org/mediawiki/2017/8/88/T--CIEI-BJ--sidebar--top.jpg" alt="side_top">
 +
</div>
 +
<ul class="page-anchors">
 +
<li><a href="#a1">Researcher Safety</a>
 +
<li><a href="#a2">Public Safety</a>
 +
<li><a href="#a3">Lab Instruments</a>
 +
<ul>
 +
<li><a href="#a4">Centrifuge</a>
 +
</li>
 +
<li><a href="#a5">Incubator</a>
 +
</li>
 +
<li><a href="#a6">Autoclave</a>
 +
</li>
 +
<li><a href="#a7">Laminar Flow</a>
 +
</li>
 +
<li><a href="#a8">Weighing Scale</a>
 +
</li>
 +
<li><a href="#a9">Gel Documentation System</a>
 +
</li>
 +
<li><a href="#a10">Gel Electrophoresis Equipment</a>
 +
</li>
 +
<li><a href="#a11">Water bath</a>
 +
</li>
 +
</ul>
 +
</ul>
 +
</div>
 +
</div>
 +
<div id="my-adjust-content">
 +
<div class="first-level" id="a1"  >Researcher Safety</div>
 +
<p class="my-content" >Everyone has to wear lab coat, rubber gloves, goggles, surgery mask when carrying out experiments.</p>
 +
<p class="my-content" >Before every experiment, our teachers have informed us about the procedure and details of every step to ensure everybody is prepared for the experiment.</p>
 +
<p class="my-content" >Everybody has to wash their hands completely before and after each experiment. In addition, everyone should use 70% alcohol to sterilize hands before entering every laminar flow.</p>
 +
<p class="my-content" >Ensuring a sterile environment for experiments: keep Eppendorf tubes and pipettes free from unneeded bacteria or impurity.</p>
 +
<p class="my-content" >Food and beverages are not allowed in labs.</p>
 +
<p class="my-content" >Power off the equipment after the experiment, avoid fire.</p>
 +
<p class="my-content" >Emergency equipment is well prepared and all of us understand clearly how to use them when we need.</p>
 +
<div class="first-level" id="a2"  >Public Safety</div>
 +
<p class="my-content" >Solid wastes should be put in solid waste cylinder, liquid wastes should be put in liquid waste cylinder.</p>
 +
<p class="my-content" >E.coli used in our experiments is harmless to human body.</p>
 +
<div class="first-level" id="a3"  >Lab Instruments</div>
 +
<div class="second-level" id="a4" >Centrifuge</div>
 +
<div class="content-wrapper">
 +
<img align="left" class="my-img" src="https://static.igem.org/mediawiki/2017/7/72/T--CIEI-BJ--Safety--fingure1.jpg" />
 +
<p class="my-content" >A centrifuge is an equipment that separates substance with different density in tubes by spinning them very quickly. It rotates its tubes in a fixed axis which is perpendicular to the ground. For example, we use it to separate plasmid and liquid, in which plasmid is heavier. When spinning in a high speed, heavier substance goes to the bottom of the tube and lighter ones go to the top. To ensure safety under a very high speed, we must place the tubes in a symmetrical position and close the lid before starting the machine.</p>
 +
</div>
 +
<div class="second-level" id="a5">Incubator</div>
 +
<div class="content-wrapper">
 +
<img class="my-img" src="https://static.igem.org/mediawiki/2017/0/01/T--CIEI-BJ--Safety--fingure2.jpg" />
 +
<p class="my-content" >Incubator can serve a best situation for the bacteria, to make bacteria grow up faster and safer. We put the E.coli which in the solid LB broth into the incubator to produce the bacteria colony, and save the E.coli sample or plasmid.</p>
 +
</div>
 +
<div class="second-level" id="a6">Autoclave</div>
 +
<div class="content-wrapper">
 +
<img class="my-img" src="https://static.igem.org/mediawiki/2017/1/1e/T--CIEI-BJ--Safety--fingure3.jpg" />
 +
<p class="my-content" >Autoclave is a big chamber with high heat and pressure to sterilize equipment. Autoclave kills bacteria and denatures or destroys proteins by subjecting them to high-temp steam. Before turning on an autoclave, the user must close the lid tightly. Although the sterilizing process only takes about 15~20 minutes, the whole heating and cooling down process takes about 1 hour.</p>
  
−
    <div class="first-level" >Researcher Safety</div>
+
</div>
−
    <p class="my-content" >Everyone has to wear lab coat, rubber gloves, goggles, surgery mask when carrying out experiments.</p>
+
<div class="second-level" id="a7">Laminar Flow</div>
−
    <p class="my-content" >Before every experiment, our teachers have informed us about the procedure and details of every step to ensure everybody is prepared for the experiment.</p>
+
<div class="content-wrapper">
−
    <p class="my-content" >Everybody has to wash their hands completely before and after each experiment. In addition, everyone should use 70% alcohol to sterilize hands before entering every laminar flow.</p>
+
<img class="my-img" src="https://static.igem.org/mediawiki/2017/b/bc/T--CIEI-BJ--Safety--fingure4.jpg" />
−
    <p class="my-content" >Ensuring a sterile environment for experiments: keep Eppendorf tubes and pipettes free from unneeded bacteria or impurity.</p>
+
<p class="my-content" >A laminar flow is an enclosed, ventilated chamber which contains UV light to kill the bacteria when not using it. Common rules about using it includes: completely sterilize your glove using 70% alcohol, turn off the UV lights when putting your hands in and avoid letting your unsterilized sleeves or watches touch the samples. Everyone must be familiar with the rules before they operate in a laminar flow.</p>
−
    <p class="my-content" >Food and beverages are not allowed in labs.</p>
+
−
    <p class="my-content" >Power off the equipment after the experiment, avoid fire.</p>
+
−
    <p class="my-content" >Emergency equipment is well prepared and all of us understand clearly how to use them when we need.</p>
+
−
    <div class="first-level" >Public Safety</div>
+
−
    <p class="my-content" >Solid wastes should be put in solid waste cylinder, liquid wastes should be put in liquid waste cylinder.</p>
+
−
    <p class="my-content" >E.coli used in our experiments is harmless to human body.</p>
+
−
    <div class="first-level" >Lab Instruments</div>
+
−
    <div class="second-level" >Centrifuge</div>
+
−
    <div class="content-wrapper">
+
−
        <img align="left" class="my-img" src="https://static.igem.org/mediawiki/2017/7/72/T--CIEI-BJ--Safety--fingure1.jpg" />
+
−
        <p class="my-content" >A centrifuge is an equipment that separates substance with different density in tubes by spinning them very quickly. It rotates its tubes in a fixed axis which is perpendicular to the ground. For example, we use it to separate plasmid and liquid, in which plasmid is heavier. When spinning in a high speed, heavier substance goes to the bottom of the tube and lighter ones go to the top. To ensure safety under a very high speed, we must place the tubes in a symmetrical position and close the lid before starting the machine.</p>
+
−
    </div>
+
−
    <div class="second-level" >Incubator</div>
+
−
    <div class="content-wrapper">
+
−
        <img class="my-img" src="https://static.igem.org/mediawiki/2017/0/01/T--CIEI-BJ--Safety--fingure2.jpg" />
+
−
        <p class="my-content" >Incubator can serve a best situation for the bacteria, to make bacteria grow up faster and safer. We put the E.coli which in the solid LB broth into the incubator to produce the bacteria colony, and save the E.coli sample or plasmid.</p>
+
−
    </div>
+
−
    <div class="second-level" >Autoclave</div>
+
−
    <div class="content-wrapper">
+
−
        <img class="my-img" src="https://static.igem.org/mediawiki/2017/1/1e/T--CIEI-BJ--Safety--fingure3.jpg" />
+
−
        <p class="my-content" >Autoclave is a big chamber with high heat and pressure to sterilize equipment. Autoclave kills bacteria and denatures or destroys proteins by subjecting them to high-temp steam. Before turning on an autoclave, the user must close the lid tightly. Although the sterilizing process only takes about 15~20 minutes, the whole heating and cooling down process takes about 1 hour.</p>
+
−
 
+
−
    </div>
+
−
    <div class="second-level" >Laminar Flow</div>
+
−
    <div class="content-wrapper">
+
−
        <img class="my-img" src="https://static.igem.org/mediawiki/2017/b/bc/T--CIEI-BJ--Safety--fingure4.jpg" />
+
−
        <p class="my-content" >A laminar flow is an enclosed, ventilated chamber which contains UV light to kill the bacteria when not using it. Common rules about using it includes: completely sterilize your glove using 70% alcohol, turn off the UV lights when putting your hands in and avoid letting your unsterilized sleeves or watches touch the samples. Everyone must be familiar with the rules before they operate in a laminar flow.</p>
+
  
−
    </div>
+
</div>
−
    <div class="second-level" >Weighing Scale</div>
+
<div class="second-level" id="a8">Weighing Scale</div>
−
    <div class="content-wrapper">
+
<div class="content-wrapper">
−
        <img class="my-img" src="https://static.igem.org/mediawiki/2017/5/51/T--CIEI-BJ--Safety--fingure5.jpg" />
+
<img class="my-img" src="https://static.igem.org/mediawiki/2017/5/51/T--CIEI-BJ--Safety--fingure5.jpg" />
−
        <p class="my-content" >Weighing scale are devices which are used to weigh the mass of some matters. It can correct the measurement to0.01g. And before we measure the weight, we need pave a paper firstly, and then place the matter on the paper,because it can protect the matter from the wind or touching.</p>
+
<p class="my-content" >Weighing scale are devices which are used to weigh the mass of some matters. It can correct the measurement to0.01g. And before we measure the weight, we need pave a paper firstly, and then place the matter on the paper,because it can protect the matter from the wind or touching.</p>
  
−
    </div>
+
</div>
−
    <div class="second-level" >Gel Documentation System</div>
+
<div class="second-level" id="a9">Gel Documentation System</div>
−
    <div class="content-wrapper">
+
<div class="content-wrapper">
−
        <img class="my-img" src="https://static.igem.org/mediawiki/2017/5/5a/T--CIEI-BJ--Safety--fingure6.jpg" />
+
<img class="my-img" src="https://static.igem.org/mediawiki/2017/5/5a/T--CIEI-BJ--Safety--fingure6.jpg" />
−
        <p class="my-content" >The gel documentation system is a huge machine that can test the trace of nucleic acid left in gel electrophoresis by exposing the whole gel to UV light. The samples are often mixed with SYBR (color).</p>
+
<p class="my-content" >The gel documentation system is a huge machine that can test the trace of nucleic acid left in gel electrophoresis by exposing the whole gel to UV light. The samples are often mixed with SYBR (color).</p>
  
−
    </div>
+
</div>
−
    <div class="second-level" >Gel Electrophoresis Equipment</div>
+
<div class="second-level" id="a10">Gel Electrophoresis Equipment</div>
−
    <div class="content-wrapper">
+
<div class="content-wrapper">
−
        <img class="my-img" src="https://static.igem.org/mediawiki/2017/3/3c/T--CIEI-BJ--Safety--fingure7.jpg" />
+
<img class="my-img" src="https://static.igem.org/mediawiki/2017/3/3c/T--CIEI-BJ--Safety--fingure7.jpg" />
−
        <p class="my-content" >Gel Electrophoresis Equipment is a machine which processes the Gel Electrophoresis. We put the gel which take the DNA and add the 1xTEA. Then we power on it. The DNA start moving from negative charge to positive charge. Because of that, we can check out the DNA whether we need. Used safe florescence dye instead of the toxic EB, wear two layers of glove, processed in specialized area, all of the waste produced were treated individually.</p>
+
<p class="my-content" >Gel Electrophoresis Equipment is a machine which processes the Gel Electrophoresis. We put the gel which take the DNA and add the 1xTEA. Then we power on it. The DNA start moving from negative charge to positive charge. Because of that, we can check out the DNA whether we need. Used safe florescence dye instead of the toxic EB, wear two layers of glove, processed in specialized area, all of the waste produced were treated individually.</p>
  
−
    </div>
+
</div>
−
    <div class="second-level" >Water bath</div>
+
<div class="second-level" id="a11">Water bath</div>
−
    <div class="content-wrapper">
+
<div class="content-wrapper">
−
        <img class="my-img" src="https://static.igem.org/mediawiki/2017/a/a3/T--CIEI-BJ--Safety--fingure8.jpg" />
+
<img class="my-img" src="https://static.igem.org/mediawiki/2017/a/a3/T--CIEI-BJ--Safety--fingure8.jpg" />
−
        <p class="my-content" >Water bath can keep a constant temperature for incubation or E.coli the water. So we can do some work which need at a high constant temperature. For example, the transformation, we put the vector and fragment on the buoy and drop into the water, then they can start transform in the best constant temperature. Furthermore, if we want to save a matter recently in a constant temperature, we also can use it. Make sure there is always water in the water bath, avoid dry heating and burning.</p>
+
<p class="my-content" >Water bath can keep a constant temperature for incubation or E.coli the water. So we can do some work which need at a high constant temperature. For example, the transformation, we put the vector and fragment on the buoy and drop into the water, then they can start transform in the best constant temperature. Furthermore, if we want to save a matter recently in a constant temperature, we also can use it. Make sure there is always water in the water bath, avoid dry heating and burning.</p>
  
−
    </div>
+
</div>
 +
</div>
 
</div>
 
</div>
 +
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{{CIEI-BJ/footer}}

Latest revision as of 13:55, 1 November 2017

backtop
Backtop
Researcher Safety

Everyone has to wear lab coat, rubber gloves, goggles, surgery mask when carrying out experiments.

Before every experiment, our teachers have informed us about the procedure and details of every step to ensure everybody is prepared for the experiment.

Everybody has to wash their hands completely before and after each experiment. In addition, everyone should use 70% alcohol to sterilize hands before entering every laminar flow.

Ensuring a sterile environment for experiments: keep Eppendorf tubes and pipettes free from unneeded bacteria or impurity.

Food and beverages are not allowed in labs.

Power off the equipment after the experiment, avoid fire.

Emergency equipment is well prepared and all of us understand clearly how to use them when we need.

Public Safety

Solid wastes should be put in solid waste cylinder, liquid wastes should be put in liquid waste cylinder.

E.coli used in our experiments is harmless to human body.

Lab Instruments
Centrifuge

A centrifuge is an equipment that separates substance with different density in tubes by spinning them very quickly. It rotates its tubes in a fixed axis which is perpendicular to the ground. For example, we use it to separate plasmid and liquid, in which plasmid is heavier. When spinning in a high speed, heavier substance goes to the bottom of the tube and lighter ones go to the top. To ensure safety under a very high speed, we must place the tubes in a symmetrical position and close the lid before starting the machine.

Incubator

Incubator can serve a best situation for the bacteria, to make bacteria grow up faster and safer. We put the E.coli which in the solid LB broth into the incubator to produce the bacteria colony, and save the E.coli sample or plasmid.

Autoclave

Autoclave is a big chamber with high heat and pressure to sterilize equipment. Autoclave kills bacteria and denatures or destroys proteins by subjecting them to high-temp steam. Before turning on an autoclave, the user must close the lid tightly. Although the sterilizing process only takes about 15~20 minutes, the whole heating and cooling down process takes about 1 hour.

Laminar Flow

A laminar flow is an enclosed, ventilated chamber which contains UV light to kill the bacteria when not using it. Common rules about using it includes: completely sterilize your glove using 70% alcohol, turn off the UV lights when putting your hands in and avoid letting your unsterilized sleeves or watches touch the samples. Everyone must be familiar with the rules before they operate in a laminar flow.

Weighing Scale

Weighing scale are devices which are used to weigh the mass of some matters. It can correct the measurement to0.01g. And before we measure the weight, we need pave a paper firstly, and then place the matter on the paper,because it can protect the matter from the wind or touching.

Gel Documentation System

The gel documentation system is a huge machine that can test the trace of nucleic acid left in gel electrophoresis by exposing the whole gel to UV light. The samples are often mixed with SYBR (color).

Gel Electrophoresis Equipment

Gel Electrophoresis Equipment is a machine which processes the Gel Electrophoresis. We put the gel which take the DNA and add the 1xTEA. Then we power on it. The DNA start moving from negative charge to positive charge. Because of that, we can check out the DNA whether we need. Used safe florescence dye instead of the toxic EB, wear two layers of glove, processed in specialized area, all of the waste produced were treated individually.

Water bath

Water bath can keep a constant temperature for incubation or E.coli the water. So we can do some work which need at a high constant temperature. For example, the transformation, we put the vector and fragment on the buoy and drop into the water, then they can start transform in the best constant temperature. Furthermore, if we want to save a matter recently in a constant temperature, we also can use it. Make sure there is always water in the water bath, avoid dry heating and burning.