Difference between revisions of "Team:ASIJ TOKYO/Description"

 
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      <a href="https://2017.igem.org/Team:ASIJ_TOKYO/Collaborations">Collaborations</a>
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       <a href="https://2017.igem.org/Team:ASIJ_TOKYO/Description">Description</a>
 
       <a href="https://2017.igem.org/Team:ASIJ_TOKYO/Description">Description</a>
 
       <a href="https://2017.igem.org/Team:ASIJ_TOKYO/Design">Design</a>
 
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       <a href="https://2017.igem.org/Team:ASIJ_TOKYO/Notebook">Notebook</a>
 
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       <a href="https://2017.igem.org/Team:ASIJ_TOKYO/Results">Results</a>
 
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   <a href="https://2017.igem.org/Team:ASIJ_TOKYO/Safety">Safety</a>
 
   <a href="https://2017.igem.org/Team:ASIJ_TOKYO/Safety">Safety</a>
 
   <a href="https://2017.igem.org/Team:ASIJ_TOKYO/Engagement">Human Practices</a>
 
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<h2 class="major"style="color:#ffffff;">Description</h2>
 
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<h2 class="major">Construct Model</h2>
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<p>Building off of a rapamycin induced split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream products, c-Myc and COX-2 (Peking iGEM Team 2015, 2015). These products are assembled into a construct composed of fusion proteins as well as split luciferase fragments (COX-2 - FRB - nLuc and c-Myc - FKBP - cLuc). In the presence of rapamycin, the interacting protein partners dimerize and subsequently cause luciferase to activate. In order to test this model, we created vectors of our constructs and inserted them into E. coli cells. </p>
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<h2 class="major">What is CRC?</h2>
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<p>Colorectal cancer (CRC), or colon/bowel cancer, is a common form of cancer that most frequently develops starting with polyps. In 2014, an estimated 1.3 million people lived with colon and rectum cancer in the United States. In fact, about 1 in 20 men and women are expected to have colon cancer at some point in their life.  
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CRC is caused by the APC gene, or activating mutation in β-catenin, which results in the accumulation of β-catenin and subsequent complex formation with TCF/LEF transcription factors. Excessive β-catenin can interact with TCF to activate the transcription of proliferative genes, such as c-MYC and cyclin D1, in the colon.</p>
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<h3 class="major">Figure 1</h3>
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<p>Our gene production construct is modeled to produce COX-2 and c-Myc, which we used to simulate conditions in human bodies when these proteins are overproduced, in the case of CRC. The construct consists of an Anderson promoter, a ribosomal binding site, a COX-2 gene/c-Myc gene, and a terminator.</p>
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<h3 class="major">Figure 2</h3>
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<p>Our promoter-reporter construct is built so that only when there is both COX-2 and c-Myc will the binding sites be able to come together and glow with the addition of rapamycin. The construct built to bind with COX-2 consists of a COX-2 promoter, FRB, n-Luc, and terminator. The construct built to bind with c-Myc consists of a c-Myc promoter, FKBP, c-Luc, and terminator.</p>
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<h3 class="major">Figure 3</h3>
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<p>Our promoter-reporter system consists of two separate constructs each with a gene-specific promoter attached to a non-specific binding site fused to a domain of split luciferase. One construct consists of a COX-2 promoter, a FRB domain, and n-Luc, and the other consists of a c-Myc promoter, a FKBP domain, and c-Luc. The split system is built so that only when the presence of both COX-2 and c-Myc is detected will the binding sites be able to come together with the addition of rapamycin and cause glowing.</p>
  
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<h3 class="major">Figure 4</h3>
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<p>In solution, our two gene constructs are able to simulate the conditions in the human body by producing proteins COX-2 and c-Myc. These proteins then interact with the promoter-reporter constructs by connecting to the non- specific binding sites, allowing the dimerization of the FKBP-Rapamycin-FRB complex. This then orients the split- luciferase so they can come together.</p>
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<h2 class="major">Project Motivations</h2>
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<p>After speaking to Minako Abe, an immunologist, we became aware of the immense prevalence and impact--especially in Japan--of colorectal cancer. In Japan alone, in 2015, there were almost 50,000 deaths from colorectal cancer, marking a sudden, sharp spike in the number of young adults that were diagnosed with this cancer. Finding out that two faculty members at our school were survivors of this disease further cemented our belief in the importance of addressing it at its root.
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The issue is especially prevalent in Japan mostly because the younger generation is not fully aware on how common colorectal cancer is becoming and because they are not checking up on their bodies as frequently as they should be. This motivated us to not limit our project on only addressing early detection of CRC, but also raising awareness.</p>
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<p>Our goal was to create a biosensor that would be able to diagnose CRC at an earlier stage and have it fit for a home-kit where use would be accessible and convenient. To do so, we looked upstream fill in. The activation of the Wnt pathway inhibits the degradation of beta-catenin, a protein that triggers the mutation of oncogenes and tumor suppressor genes. Building off of a rapamycin induced split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream products, C-myc and COX-2.
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As for community outreach, we wanted to take advantage of the fact that we live in the most metropolitan area in Japan. We did so by carrying out our project mainly in Harajuku, which is one of the most popular areas in Tokyo. As ASIJ is also central to many international communities, we’ve also promoted our project at family events held at ASIJ, as well as through our virtual pre-medical club where we were able to interact with schools around the globe.</p>
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box-shadow: inset 0 -1px 0 0 #f2efe8, 0 1px 0 0 #f2efe8;
 +
}
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 +
.wrapper.style3 {
 +
background-color: #f2efe8;
 +
}
 +
 
 +
.wrapper.style3:before, .wrapper.style3:after {
 +
 
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}
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 +
.wrapper.style3:before {
 +
box-shadow: inset 0 -1px 0 0 #f2efe8, 0 1px 0 0 #f2efe8;
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 +
.wrapper.style3:after {
 +
box-shadow: inset 0 -1px 0 0 #f2efe8, 0 1px 0 0 #f2efe8;
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}
 +
 
 +
.wrapper.style4 {
 +
background-color: #dbe9d8;
 +
}
 +
 
 +
.wrapper.style4:before, .wrapper.style4:after {
 +
 
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}
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.wrapper.style4:before {
 +
box-shadow: inset 0 -1px 0 0 #dbe9d8, 0 1px 0 0 #dbe9d8;
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.wrapper.style4:after {
 +
box-shadow: inset 0 -1px 0 0 #dbe9d8, 0 1px 0 0 #dbe9d8;
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}
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 +
.wrapper.style5 {
 +
background-color: #dbe9d8;
 +
}
 +
 
 +
.wrapper.style5:before, .wrapper.style5:after {
 +
 
 +
}
 +
 
 +
.wrapper.style5:before {
 +
box-shadow: inset 0 -1px 0 0 #dbe9d8, 0 1px 0 0 #dbe9d8;
 +
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 +
.wrapper.style5:after {
 +
box-shadow: inset 0 -1px 0 0 #dbe9d8, 0 1px 0 0 #dbe9d8;
 +
}
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 +
.wrapper.style6 {
 +
background-color: #555867;
 +
}
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 +
.wrapper.style6:before, .wrapper.style6:after {
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}
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 +
.wrapper.style6:before {
 +
box-shadow: inset 0 -1px 0 0 #555867, 0 1px 0 0 #555867;
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 +
.wrapper.style6:after {
 +
box-shadow: inset 0 -1px 0 0 #555867, 0 1px 0 0 #555867;
 +
}
 +
 
 +
.wrapper.spotlight {
 +
background-color: #dbe9d8;
 +
}
 +
 
 +
.wrapper.spotlight:before, .wrapper.spotlight:after {
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}
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 +
.wrapper.spotlight:before {
 +
box-shadow: inset 0 -1px 0 0 #dbe9d8, 0 1px 0 0 #dbe9d8;
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}
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 +
.wrapper.spotlight:after {
 +
box-shadow: inset 0 -1px 0 0 #dbe9d8, 0 1px 0 0 #dbe9d8;
 +
}
 +
 
 +
.wrapper.spotlight .inner {
 +
display: -moz-flex;
 +
display: -webkit-flex;
 +
display: -ms-flex;
 +
display: flex;
 +
-moz-align-items: center;
 +
-webkit-align-items: center;
 +
-ms-align-items: center;
 +
align-items: center;
 +
-moz-flex-direction: row;
 +
-webkit-flex-direction: row;
 +
-ms-flex-direction: row;
 +
flex-direction: row;
 +
}
 +
 
 +
.wrapper.spotlight .image {
 +
border-radius: 100%;
 +
margin: 0 3em 2em 0;
 +
width: 22em;
 +
overflow: hidden;
 +
-ms-flex: 1;
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}
 +
 
 +
.wrapper.spotlight .image img {
 +
border-radius: 100%;
 +
width: 100%;
 +
}
 +
 
 +
.wrapper.spotlight .content {
 +
width: 100%;
 +
-ms-flex: 2;
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}
 +
 
 +
.wrapper.spotlight:nth-child(2n - 1) .inner {
 +
-moz-flex-direction: row-reverse;
 +
-webkit-flex-direction: row-reverse;
 +
-ms-flex-direction: row-reverse;
 +
flex-direction: row-reverse;
 +
text-align: right;
 +
}
 +
 
 +
.wrapper.spotlight:nth-child(2n - 1) .image {
 +
margin: 0 0 2em 3em;
 +
}
 +
 
 +
.wrapper.spotlight.style2 {
 +
background-color: #dbe9d8;
 +
}
 +
 
 +
.wrapper.spotlight.style2:before, .wrapper.spotlight.style2:after {
 +
 
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}
 +
 
 +
.wrapper.spotlight.style2:before {
 +
box-shadow: inset 0 -1px 0 0 #dbe9d8, 0 1px 0 0 #dbe9d8;
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 +
.wrapper.spotlight.style2:after {
 +
box-shadow: inset 0 -1px 0 0 #dbe9d8, 0 1px 0 0 #dbe9d8;
 +
}
 +
 
 +
.wrapper.spotlight.style3 {
 +
background-color: #f2efe8;
 +
}
 +
 
 +
.wrapper.spotlight.style3:before, .wrapper.spotlight.style3:after {
 +
 
 +
}
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 +
.wrapper.spotlight.style3:before {
 +
box-shadow: inset 0 -1px 0 0 #f2efe8, 0 1px 0 0 #f2efe8;
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 +
.wrapper.spotlight.style3:after {
 +
box-shadow: inset 0 -1px 0 0 #f2efe8, 0 1px 0 0 #f2efe8;
 +
}
 +
 
 +
.wrapper.spotlight.style4 {
 +
background-color: #f2efe8;
 +
}
 +
 
 +
.wrapper.spotlight.style4:before, .wrapper.spotlight.style4:after {
 +
 
 +
}
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 +
.wrapper.spotlight.style4:before {
 +
box-shadow: inset 0 -1px 0 0 #f2efe8, 0 1px 0 0 #f2efe8;
 +
}
 +
 
 +
.wrapper.spotlight.style4:after {
 +
box-shadow: inset 0 -1px 0 0 #f2efe8, 0 1px 0 0 #f2efe8;
 +
}
 +
 
 +
.wrapper.spotlight.style5 {
 +
background-color: #f2efe8;
 +
}
 +
 
 +
.wrapper.spotlight.style5:before, .wrapper.spotlight.style5:after {
 +
 
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}
 +
 
 +
.wrapper.spotlight.style5:before {
 +
box-shadow: inset 0 -1px 0 0 #f2efe8, 0 1px 0 0 #f2efe8;
 +
}
 +
 
 +
.wrapper.spotlight.style5:after {
 +
box-shadow: inset 0 -1px 0 0 #f2efe8, 0 1px 0 0 #f2efe8;
 +
}
 +
 
 +
.wrapper.spotlight.style6 {
 +
background-color: #2d3a69;
 +
}
 +
 
 +
.wrapper.spotlight.style6:before, .wrapper.spotlight.style6:after {
 +
 
 +
}
 +
 
 +
.wrapper.spotlight.style6:before {
 +
box-shadow: inset 0 -1px 0 0 #2d3a69, 0 1px 0 0 #2d3a69;
 +
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 +
 
 +
.wrapper.spotlight.style6:after {
 +
box-shadow: inset 0 -1px 0 0 #2d3a69, 0 1px 0 0 #2d3a69;
 +
}
 +
 
 +
@media screen and (max-width: 980px) {
 +
 
 +
.wrapper {
 +
margin: 4.75em 0;
 +
}
 +
 
 +
.wrapper:before, .wrapper:after {
 +
height: 4.75em;
 +
}
 +
 
 +
.wrapper:before {
 +
top: -4.75em;
 +
}
 +
 
 +
.wrapper:after {
 +
bottom: -4.75em;
 +
left: 0;
 +
}
 +
 
 +
.wrapper .inner {
 +
padding: 3em 3em 1em 3em ;
 +
width: 100%;
 +
}
 +
 
 +
.wrapper.spotlight .image {
 +
margin: 0 2em 2em 0;
 +
width: 32em;
 +
}
 +
 
 +
.wrapper.spotlight:nth-child(2n - 1) .image {
 +
margin: 0 0 2em 2em;
 +
}
 +
 
 +
}
 +
 
 +
@media screen and (max-width: 736px) {
 +
 
 +
.wrapper {
 +
margin: 2.5em 0;
 +
}
 +
 
 +
.wrapper:before, .wrapper:after {
 +
height: 2.5em;
 +
}
 +
 
 +
.wrapper:before {
 +
top: -2.5em;
 +
}
 +
 
 +
.wrapper:after {
 +
bottom: -2.5em;
 +
left: 0;
 +
}
 +
 
 +
.wrapper .inner {
 +
padding: 2em 2em 0.1em 2em ;
 +
}
 +
 
 +
.wrapper.spotlight .inner {
 +
-moz-align-items: -moz-flex-start;
 +
-webkit-align-items: -webkit-flex-start;
 +
-ms-align-items: -ms-flex-start;
 +
align-items: flex-start;
 +
}
 +
 
 +
.wrapper.spotlight .image {
 +
width: 19em;
 +
margin: 0 1.75em 2em 0;
 +
}
 +
 
 +
.wrapper.spotlight:nth-child(2n - 1) .image {
 +
margin: 0 0 2em 1.75em;
 +
}
 +
 
 +
}
 +
 
 +
@media screen and (max-width: 640px) {
 +
 
 +
.wrapper.spotlight .inner {
 +
display: block;
 +
}
 +
 
 +
.wrapper.spotlight .image {
 +
margin: 0 0 1em 0 !important;
 +
max-width: 85%;
 +
width: 12em;
 +
}
 +
 
 +
}
 +
 
 +
@media screen and (max-width: 360px) {
 +
 
 +
.wrapper .inner {
 +
padding: 2em 1.5em 0.1em 1.5em ;
 +
}
 +
 
 +
}
 +
 
 +
/* Footer */
 +
 
 +
#footer .inner {
 +
padding: 5em 0 3em 0 ;
 +
display: -moz-flex;
 +
display: -webkit-flex;
 +
display: -ms-flex;
 +
display: flex;
 +
-moz-flex-direction: row;
 +
-webkit-flex-direction: row;
 +
-ms-flex-direction: row;
 +
flex-direction: row;
 +
-moz-flex-wrap: wrap;
 +
-webkit-flex-wrap: wrap;
 +
-ms-flex-wrap: wrap;
 +
flex-wrap: wrap;
 +
margin: 0 auto;
 +
width: 55em;
 +
}
 +
 
 +
#footer .inner > * {
 +
width: 100%;
 +
}
 +
 
 +
#footer .inner form {
 +
margin: 0 3em 0 0;
 +
width: calc(50% - 1.5em);
 +
}
 +
 
 +
#footer .inner .contact {
 +
width: calc(50% - 1.5em);
 +
}
 +
 
 +
#footer .inner .copyright {
 +
border-top: solid 2px rgba(255, 255, 255, 0.125);
 +
list-style: none;
 +
margin: 4em 0 2em 0;
 +
padding: 2em 0 0 0;
 +
width: 100%;
 +
}
 +
 
 +
#footer .inner .copyright li {
 +
border-left: solid 2px rgba(255, 255, 255, 0.125);
 +
color: rgba(255, 255, 255, 0.35);
 +
display: inline-block;
 +
font-size: 0.9em;
 +
line-height: 1;
 +
margin-left: 1em;
 +
padding: 0;
 +
padding-left: 1em;
 +
}
 +
 
 +
#footer .inner .copyright li:first-child {
 +
border-left: 0;
 +
margin-left: 0;
 +
padding-left: 0;
 +
}
 +
 
 +
#footer .inner .copyright li a {
 +
color: inherit;
 +
}
 +
 
 +
@media screen and (max-width: 1280px) {
 +
 
 +
#footer {
 +
background-color: #dbe9d8;
 +
background-image: linear-gradient(to top, rgba(46, 49, 65, 0.8), rgba(46, 49, 65, 0.8)), url("../../images/bg.jpg");
 +
background-size: auto, cover;
 +
background-position: center, center;
 +
margin-top: -6.5em;
 +
padding-top: 6.5em;
 +
}
 +
 
 +
}
 +
 
 +
@media screen and (max-width: 980px) {
 +
 
 +
#footer {
 +
margin-top: -4.75em;
 +
padding-top: 4.75em;
 +
}
 +
 
 +
#footer .inner {
 +
padding: 3em 3em 1em 3em ;
 +
display: block;
 +
width: 100%;
 +
}
 +
 
 +
#footer .inner form {
 +
width: 100%;
 +
margin: 0 0 4em 0;
 +
}
 +
 
 +
#footer .inner .contact {
 +
width: 100%;
 +
margin: 0 0 4em 0;
 +
}
 +
 
 +
#footer .inner .copyright {
 +
margin: 4em 0 2em 0;
 +
}
 +
 
 +
}
 +
 
 +
@media screen and (max-width: 736px) {
 +
 
 +
#footer {
 +
margin-top: -2.5em;
 +
padding-top: 2.5em;
 +
}
 +
 
 +
#footer .inner {
 +
padding: 2em 2em 0.1em 2em ;
 +
}
 +
 
 +
#footer .inner form {
 +
margin: 0 0 3em 0;
 +
}
 +
 
 +
#footer .inner .contact {
 +
margin: 0 0 3em 0;
 +
}
 +
 
 +
}
 +
 
 +
@media screen and (max-width: 640px) {
 +
 
 +
#footer .inner .copyright li {
 +
border-left: 0;
 +
display: block;
 +
margin: 1em 0 0 0;
 +
padding-left: 0;
 +
}
 +
 
 +
#footer .inner .copyright li:first-child {
 +
margin-top: 0;
 +
}
 +
 
 +
}
 +
 
 +
@media screen and (max-width: 360px) {
 +
 
 +
#footer .inner {
 +
padding: 2em 1.5em 0.1em 1.5em ;
 +
}
 +
 
 +
}
 +
</style>
 +
<style>
 +
/*
 +
Solid State by HTML5 UP
 +
html5up.net | @ajlkn
 +
Free for personal and commercial use under the CCA 3.0 license (html5up.net/license)
 +
*/
 +
 
 +
/* Basic */
 +
 
 +
body {
 +
background-color: #dbe9d8;
 +
background-image: url("../../images/bg.jpg");
 +
background-size: cover;
 +
background-attachment: fixed;
 +
background-position: center;
 +
}
 +
 
 +
body:before {
 +
background: rgba(46, 49, 65, 0.8);
 +
content: '';
 +
display: block;
 +
height: 100%;
 +
left: 0;
 +
position: fixed;
 +
top: 0;
 +
width: 100%;
 +
}
 +
 
 +
body > * {
 +
position: relative;
 +
z-index: 1;
 +
}
 +
 
 +
/* Features */
 +
 
 +
.features article {
 +
display: inline-block;
 +
width: 45%;
 +
}
 +
 
 +
/* Menu */
 +
 
 +
#menu .inner {
 +
margin: 4em auto;
 +
}
 +
 
 +
/* Wrapper */
 +
 
 +
#wrapper > header {
 +
background: none !important;
 +
}
 +
 
 +
.wrapper.spotlight .inner {
 +
text-align: left !important;
 +
}
 +
 
 +
.wrapper.spotlight .image {
 +
display: inline-block;
 +
margin: 0 3em 2em 0 !important;
 +
vertical-align: middle;
 +
width: 24%;
 +
}
 +
 
 +
.wrapper.spotlight .content {
 +
display: inline-block;
 +
vertical-align: middle;
 +
width: 70%;
 +
}
 +
 
 +
/* Banner */
 +
 
 +
#banner {
 +
background: none !important;
 +
}
 +
 
 +
/* Footer */
 +
 
 +
#footer {
 +
background: none !important;
 +
}
 +
 
 +
#footer .inner form {
 +
display: inline-block;
 +
width: 45%;
 +
}
 +
 
 +
#footer .inner .contact {
 +
display: inline-block;
 +
width: 45%;
 +
}
 +
</style>
 +
<style>
 +
/*
 +
Solid State by HTML5 UP
 +
html5up.net | @ajlkn
 +
Free for personal and commercial use under the CCA 3.0 license (html5up.net/license)
 +
*/
 +
 
 +
/* Basic */
 +
 
 +
body {
 +
-ms-behavior: url("assets/js/backgroundsize.min.htc");
 +
}
 +
 
 +
/* Type */
 +
 
 +
h1.major, h2.major, h3.major, h4.major, h5.major, h6.major {
 +
border-bottom: solid 2px #ffffff;
 +
}
 +
 
 +
blockquote {
 +
border-left: solid 4px #ffffff;
 +
}
 +
 
 +
code {
 +
border: solid 2px #ffffff;
 +
}
 +
 
 +
hr {
 +
border-bottom: solid 2px #ffffff;
 +
}
 +
 
 +
/* Button */
 +
 
 +
input[type="submit"],
 +
input[type="reset"],
 +
input[type="button"],
 +
button,
 +
.button {
 +
position: relative;
 +
-ms-behavior: url("assets/js/ie/PIE.htc");
 +
border: solid 2px #ffffff;
 +
}
 +
 
 +
input[type="submit"].special,
 +
input[type="reset"].special,
 +
input[type="button"].special,
 +
button.special,
 +
.button.special {
 +
border: 0;
 +
}
 +
 
 +
/* Form */
 +
 
 +
input[type="text"],
 +
input[type="password"],
 +
input[type="email"],
 +
input[type="tel"],
 +
select,
 +
textarea {
 +
position: relative;
 +
-ms-behavior: url("assets/js/ie/PIE.htc");
 +
background: transparent;
 +
border: solid 2px #ffffff;
 +
}
 +
 
 +
/* Table */
 +
 
 +
table tbody tr {
 +
border: solid 1px #ffffff;
 +
border-left: 0;
 +
border-right: 0;
 +
}
 +
 
 +
table.alt tbody tr td {
 +
border: solid 1px #ffffff;
 +
border-left-width: 0;
 +
border-top-width: 0;
 +
}
 +
 
 +
table.alt tbody tr td:first-child {
 +
border-left-width: 1px;
 +
}
 +
 
 +
table.alt tbody tr:first-child td {
 +
border-top-width: 1px;
 +
}
 +
 
 +
table.alt thead {
 +
border-bottom: 0;
 +
}
 +
 
 +
table.alt tfoot {
 +
border-top: 0;
 +
}
 +
 
 +
/* Features */
 +
 
 +
.features article {
 +
-ms-behavior: url("assets/js/ie/PIE.htc");
 +
position: relative;
 +
width: 44%;
 +
}
 +
 
 +
.features article .image {
 +
margin-top: 0;
 +
margin-left: 0;
 +
width: 100%;
 +
}
 +
 
 +
/* Menu */
 +
 
 +
#menu {
 +
background: #dbe9d8;
 +
}
 +
 
 +
#menu h2 {
 +
border-bottom: solid 2px #ffffff;
 +
}
 +
 
 +
/* Header */
 +
 
 +
#header {
 +
background-color: #f2efe8;
 +
}
 +
 
 +
/* Wrapper */
 +
 
 +
.wrapper {
 +
margin: 0;
 +
}
 +
 
 +
.wrapper:before, .wrapper:after {
 +
display: none;
 +
}
 +
 
 +
.wrapper.spotlight .image {
 +
-ms-behavior: url("assets/js/ie/PIE.htc");
 +
}
 +
 
 +
.wrapper.spotlight .image img {
 +
position: relative;
 +
-ms-behavior: url("assets/js/ie/PIE.htc");
 +
}
 +
 
 +
/* Banner */
 +
 
 +
#banner .logo .icon {
 +
border: solid 2px #ffffff;
 +
-ms-behavior: url("assets/js/ie/PIE.htc");
 +
}
 +
 
 +
#banner h2 {
 +
border-bottom: solid 2px #ffffff;
 +
}
 +
 
 +
/* Footer */
 +
 
 +
#footer .inner .copyright {
 +
border-top: solid 2px #ffffff;
 +
}
 +
 
 +
#footer .inner .copyright li {
 +
border-left: solid 2px #ffffff;
 +
}
 +
</style>
 +
 
 +
 
 +
 
 +
</body>
 +
</html>
 +
p

Latest revision as of 15:53, 1 November 2017

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Parts Safety Human Practices

Description

Construct Model

Building off of a rapamycin induced split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream products, c-Myc and COX-2 (Peking iGEM Team 2015, 2015). These products are assembled into a construct composed of fusion proteins as well as split luciferase fragments (COX-2 - FRB - nLuc and c-Myc - FKBP - cLuc). In the presence of rapamycin, the interacting protein partners dimerize and subsequently cause luciferase to activate. In order to test this model, we created vectors of our constructs and inserted them into E. coli cells.

Figure 1

Our gene production construct is modeled to produce COX-2 and c-Myc, which we used to simulate conditions in human bodies when these proteins are overproduced, in the case of CRC. The construct consists of an Anderson promoter, a ribosomal binding site, a COX-2 gene/c-Myc gene, and a terminator.

Figure 2

Our promoter-reporter construct is built so that only when there is both COX-2 and c-Myc will the binding sites be able to come together and glow with the addition of rapamycin. The construct built to bind with COX-2 consists of a COX-2 promoter, FRB, n-Luc, and terminator. The construct built to bind with c-Myc consists of a c-Myc promoter, FKBP, c-Luc, and terminator.

Figure 3

Our promoter-reporter system consists of two separate constructs each with a gene-specific promoter attached to a non-specific binding site fused to a domain of split luciferase. One construct consists of a COX-2 promoter, a FRB domain, and n-Luc, and the other consists of a c-Myc promoter, a FKBP domain, and c-Luc. The split system is built so that only when the presence of both COX-2 and c-Myc is detected will the binding sites be able to come together with the addition of rapamycin and cause glowing.

Figure 4

In solution, our two gene constructs are able to simulate the conditions in the human body by producing proteins COX-2 and c-Myc. These proteins then interact with the promoter-reporter constructs by connecting to the non- specific binding sites, allowing the dimerization of the FKBP-Rapamycin-FRB complex. This then orients the split- luciferase so they can come together.

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