|
|
(39 intermediate revisions by 6 users not shown) |
Line 8: |
Line 8: |
| <head> | | <head> |
| | | |
− | <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css">
| + | |
| | | |
| <style> | | <style> |
Line 28: |
Line 28: |
| float: left; | | float: left; |
| color: #858687; | | color: #858687; |
− | text-align: center; | + | text-align: left; |
| padding: 10px 32px; | | padding: 10px 32px; |
| text-decoration: none; | | text-decoration: none; |
Line 131: |
Line 131: |
| | | |
| | | |
− | | + | <!-- Three --> |
− | | + | <section id="three" class="wrapper spotlight style2"> |
− | | + | |
− | <!-- Two -->
| + | |
− | <section id=“three” class="wrapper spotlight style3”> | + | |
| <div class="inner"> | | <div class="inner"> |
− | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/1/18/Asij-ttpres1.jpg" alt="" /></a> | + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/3/31/Anchiegg.png" alt="" /></a> |
| <div class="content"> | | <div class="content"> |
− | <h2 class="major">Description</h2> | + | <h2 class="major"style="color:#ffffff;">Description</h2> |
| </div> | | </div> |
| </div> | | </div> |
| </section> | | </section> |
− |
| |
| | | |
| <!-- Three --> | | <!-- Three --> |
− | <section id="two" class="wrapper spotlight style4"> | + | <section id="four" class="wrapper spotlight style1"> |
| <div class="inner"> | | <div class="inner"> |
| <div class="content"> | | <div class="content"> |
| <h2 class="major">Construct Model</h2> | | <h2 class="major">Construct Model</h2> |
− | <p>Building off of a rapamycin induced split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream products, c-Myc and COX-2 (Peking iGEM Team 2015, 2015). These products are assembled into a construct composing of fusion proteins as well as split luciferase fragments (COX-2 - nLuc - FRB and FKBP - cLuc - c-Myc). In the presence of rapamycin, the interacting protein partners dimerize and subsequently cause luciferase to activate. In order to test this model, we created vectors of our constructs and inserted them into e-coli cells. </p> | + | <p>Building off of a rapamycin induced split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream products, c-Myc and COX-2 (Peking iGEM Team 2015, 2015). These products are assembled into a construct composed of fusion proteins as well as split luciferase fragments (COX-2 - FRB - nLuc and c-Myc - FKBP - cLuc). In the presence of rapamycin, the interacting protein partners dimerize and subsequently cause luciferase to activate. In order to test this model, we created vectors of our constructs and inserted them into E. coli cells. </p> |
| </div> | | </div> |
| </div> | | </div> |
Line 158: |
Line 154: |
| <!-- Four --> | | <!-- Four --> |
| <section id="four" class="wrapper alt style1"> | | <section id="four" class="wrapper alt style1"> |
− | <div class="inner">
| + | <div class="inner"> |
− | <h2 class="major">Composite Parts</h2>
| + | |
| <section class="features"> | | <section class="features"> |
| <article> | | <article> |
− | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/0/03/Diagram_Models_%283%29.jpg" title="Click to return back to the top." /></a> | + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/c/cc/Diagram_Models_%283%29-1.jpg" /></a> |
| <h3 class="major">Figure 1</h3> | | <h3 class="major">Figure 1</h3> |
| <p>Our gene production construct is modeled to produce COX-2 and c-Myc, which we used to simulate conditions in human bodies when these proteins are overproduced, in the case of CRC. The construct consists of an Anderson promoter, a ribosomal binding site, a COX-2 gene/c-Myc gene, and a terminator.</p> | | <p>Our gene production construct is modeled to produce COX-2 and c-Myc, which we used to simulate conditions in human bodies when these proteins are overproduced, in the case of CRC. The construct consists of an Anderson promoter, a ribosomal binding site, a COX-2 gene/c-Myc gene, and a terminator.</p> |
| </article> | | </article> |
| <article> | | <article> |
− | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/8/85/Diagram_Models_%281%29.jpg" title="Click to return back to the top." /></a> | + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/9/90/P-Rsystemanchiegg.jpg" title="Click to return back to the top." /></a> |
| <h3 class="major">Figure 2</h3> | | <h3 class="major">Figure 2</h3> |
− | <p>Our promoter-reporter construct is built so that only when there is both COX-2 and c-Myc will the binding sites be able to come together and glow with the addition of rapamycin. The construct built to bind with COX-2 consists of a COX-2 promoter, FKBP, c-Luc, and terminator. The construct built to bind with c-Myc consists of a c-Myc promoter, FRB, n-Luc, and terminator.</p> | + | <p>Our promoter-reporter construct is built so that only when there is both COX-2 and c-Myc will the binding sites be able to come together and glow with the addition of rapamycin. The construct built to bind with COX-2 consists of a COX-2 promoter, FRB, n-Luc, and terminator. The construct built to bind with c-Myc consists of a c-Myc promoter, FKBP, c-Luc, and terminator.</p> |
| </article> | | </article> |
| <article> | | <article> |
− | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/d/dc/Diagram_Models_.jpg" title="Click to return back to the top." /></a> | + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/a/a5/P-Rsystemanchiegg2.jpg" title="Click to return back to the top." /></a> |
| <h3 class="major">Figure 3</h3> | | <h3 class="major">Figure 3</h3> |
− | <p>Our promoter-reporter construct is built so that only when there is both COX-2 and c-Myc will the binding sites be able to come together and glow with the addition of rapamycin. The construct built to bind with COX-2 consists of a COX-2 promoter, FKBP, c-Luc, and terminator. The construct built to bind with c-Myc consists of a c-Myc promoter, FRB, n-Luc, and terminator.</p> | + | <p>Our promoter-reporter system consists of two separate constructs each with a gene-specific promoter attached to a non-specific binding site fused to a domain of split luciferase. One construct consists of a COX-2 promoter, a FRB domain, and n-Luc, and the other consists of a c-Myc promoter, a FKBP domain, and c-Luc. The split system is built so that only when the presence of both COX-2 and c-Myc is detected will the binding sites be able to come together with the addition of rapamycin and cause glowing.</p> |
| | | |
| </article> | | </article> |
| <article> | | <article> |
| | | |
− | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/3/3a/Diagram_Models_%282%29.jpg" title="Click to return back to the top." /></a> | + | <a href="#" class="image"><img src="https://static.igem.org/mediawiki/2017/e/ec/Insolutionanchiegg.jpg" title="Click to return back to the top." /></a> |
| <h3 class="major">Figure 4</h3> | | <h3 class="major">Figure 4</h3> |
| <p>In solution, our two gene constructs are able to simulate the conditions in the human body by producing proteins COX-2 and c-Myc. These proteins then interact with the promoter-reporter constructs by connecting to the non- specific binding sites, allowing the dimerization of the FKBP-Rapamycin-FRB complex. This then orients the split- luciferase so they can come together.</p> | | <p>In solution, our two gene constructs are able to simulate the conditions in the human body by producing proteins COX-2 and c-Myc. These proteins then interact with the promoter-reporter constructs by connecting to the non- specific binding sites, allowing the dimerization of the FKBP-Rapamycin-FRB complex. This then orients the split- luciferase so they can come together.</p> |
Line 189: |
Line 185: |
| | | |
| | | |
− |
| + | |
− | <!-- Scripts --> | + | |
− | <script src="assets/js/skel.min.js"></script>
| + | |
− | <script src="assets/js/jquery.min.js"></script>
| + | |
− | <script src="assets/js/jquery.scrollex.min.js"></script>
| + | |
− | <script src="assets/js/util.js"></script>
| + | |
− | <!--[if lte IE 8]><script src="assets/js/ie/respond.min.js"></script><![endif]-->
| + | |
− | <script src="assets/js/main.js"></script>
| + | |
− | | + | |
− | | + | |
| <style> | | <style> |
| @import url(font-awesome.min.css); | | @import url(font-awesome.min.css); |
− | @import url("https://fonts.googleapis.com/css?family=Raleway:200,700|Source+Sans+Pro:300,600,300italic,600italic");
| + | |
| | | |
| /* | | /* |
Line 211: |
Line 198: |
| /* Reset */ | | /* Reset */ |
| | | |
− | html, body, div, span, applet, object, iframe, h1, h2, h3, h4, h5, h6, p, blockquote, pre, a, abbr, acronym, address, big, cite, code, del, dfn, em, img, ins, kbd, q, s, samp, small, strike, strong, sub, sup, tt, var, b, u, i, center, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td, article, aside, canvas, details, embed, figure, figcaption, footer, header, hgroup, menu, nav, output, ruby, section, summary, time, mark, audio, video { | + | html, body, div, span, applet, object, h1, h2, h3, h4, h5, h6, p, blockquote, pre, a, abbr, acronym, address, big, cite, code, del, dfn, em, img, ins, kbd, q, s, samp, small, strike, strong, sub, sup, tt, var, b, u, i, center, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td, article, aside, canvas, details, embed, figure, figcaption, footer, header, hgroup, menu, nav, output, ruby, section, summary, time, mark, audio, video { |
| margin: 0; | | margin: 0; |
| padding: 0; | | padding: 0; |
Line 2,086: |
Line 2,073: |
| | | |
| .align-right { | | .align-right { |
− | text-align: right; | + | text-align: left; |
| } | | } |
| | | |
Line 2,679: |
Line 2,666: |
| ul.pagination li:last-child { | | ul.pagination li:last-child { |
| width: calc(50% - 2px); | | width: calc(50% - 2px); |
− | text-align: right; | + | text-align: left; |
| padding-left: 0.325em; | | padding-left: 0.325em; |
| } | | } |
Line 3,135: |
Line 3,122: |
| left: 0; | | left: 0; |
| text-indent: 0; | | text-indent: 0; |
− | text-align: right; | + | text-align: left; |
| margin-left: 0; | | margin-left: 0; |
| padding-right: 1.25em; | | padding-right: 1.25em; |
Line 3,546: |
Line 3,533: |
| | | |
| .wrapper:before, .wrapper:after { | | .wrapper:before, .wrapper:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%232e3141%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,603: |
Line 3,590: |
| .wrapper.style2 { | | .wrapper.style2 { |
| background-color: #f2efe8; | | background-color: #f2efe8; |
| + | background-image: url("https://static.igem.org/mediawiki/2017/3/32/Descriptionthingasij.png") |
| } | | } |
| | | |
| .wrapper.style2:before, .wrapper.style2:after { | | .wrapper.style2:before, .wrapper.style2:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%23353849%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,622: |
Line 3,610: |
| | | |
| .wrapper.style3:before, .wrapper.style3:after { | | .wrapper.style3:before, .wrapper.style3:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%233d4051%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,638: |
Line 3,626: |
| | | |
| .wrapper.style4:before, .wrapper.style4:after { | | .wrapper.style4:before, .wrapper.style4:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%23454858%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,654: |
Line 3,642: |
| | | |
| .wrapper.style5:before, .wrapper.style5:after { | | .wrapper.style5:before, .wrapper.style5:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%234d5060%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,670: |
Line 3,658: |
| | | |
| .wrapper.style6:before, .wrapper.style6:after { | | .wrapper.style6:before, .wrapper.style6:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%23555867%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,686: |
Line 3,674: |
| | | |
| .wrapper.spotlight:before, .wrapper.spotlight:after { | | .wrapper.spotlight:before, .wrapper.spotlight:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%234c5c96%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,747: |
Line 3,735: |
| | | |
| .wrapper.spotlight.style2:before, .wrapper.spotlight.style2:after { | | .wrapper.spotlight.style2:before, .wrapper.spotlight.style2:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%2345558d%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,763: |
Line 3,751: |
| | | |
| .wrapper.spotlight.style3:before, .wrapper.spotlight.style3:after { | | .wrapper.spotlight.style3:before, .wrapper.spotlight.style3:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%233f4e85%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,779: |
Line 3,767: |
| | | |
| .wrapper.spotlight.style4:before, .wrapper.spotlight.style4:after { | | .wrapper.spotlight.style4:before, .wrapper.spotlight.style4:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%2339477c%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,795: |
Line 3,783: |
| | | |
| .wrapper.spotlight.style5:before, .wrapper.spotlight.style5:after { | | .wrapper.spotlight.style5:before, .wrapper.spotlight.style5:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%23324072%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 3,811: |
Line 3,799: |
| | | |
| .wrapper.spotlight.style6:before, .wrapper.spotlight.style6:after { | | .wrapper.spotlight.style6:before, .wrapper.spotlight.style6:after { |
− | background-image: url("data:image/svg+xml;charset=utf8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='100' height='100' viewBox='0 0 100 100' preserveAspectRatio='none'%3E%3Cpolygon points='0,100 100,0 100,100' style='fill:%232d3a69%3B' /%3E%3C/svg%3E");
| + | |
| } | | } |
| | | |
Line 4,329: |
Line 4,317: |
| </body> | | </body> |
| </html> | | </html> |
| + | p |