Difference between revisions of "Team:UCLouvain/Basic Part"
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<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | <span> | + | <span>Parts we created</span> |
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| + | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| + | <p class="montserrat-text uppercase">ComR (BBa_K2341000)</p> | ||
| + | </div> | ||
<p>Protein originally found in <i>Streptococcus thermophilius</i> where it regulates natural competence. The protein activation and binding to specific promoters (ComR Box; BBa_K2341001) is only possible in presence of a short peptide, ComS (LPYFAGCL). Binding to the promoter will induce the downstream sequence expression.</p> | <p>Protein originally found in <i>Streptococcus thermophilius</i> where it regulates natural competence. The protein activation and binding to specific promoters (ComR Box; BBa_K2341001) is only possible in presence of a short peptide, ComS (LPYFAGCL). Binding to the promoter will induce the downstream sequence expression.</p> | ||
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<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | < | + | <p class="montserrat-text uppercase">P1655 promoter (BBa_K2341001)</p> |
</div> | </div> | ||
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<p>Promote expression of the sequence downstream in presence of ComR (BBa_K2341000) and ComS (LPYFAGCL). Sequence containing the palindrome motif needed to bind ComR (= ComR box).</p> | <p>Promote expression of the sequence downstream in presence of ComR (BBa_K2341000) and ComS (LPYFAGCL). Sequence containing the palindrome motif needed to bind ComR (= ComR box).</p> | ||
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<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | < | + | <p class="montserrat-text uppercase">sgRNA Tyrosin (Cas9 guide) (BBa_K2341100)</p> |
</div> | </div> | ||
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<p>RNA guide to use in combination with CRISPR/CAS9. Cut inside the tyrA gene so that deletion by homologous recombination can occur if a homologous DNA strand is also added. RNA guide under constitutive expression by BBa_J23119.</p> | <p>RNA guide to use in combination with CRISPR/CAS9. Cut inside the tyrA gene so that deletion by homologous recombination can occur if a homologous DNA strand is also added. RNA guide under constitutive expression by BBa_J23119.</p> | ||
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<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | <span> | + | <span>Parts we used</span> |
</div> | </div> | ||
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| + | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| + | <p class="montserrat-text uppercase">pBAD/araC (I0500)</p> | ||
| + | </div> | ||
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<p>The pBAD promoter is a very common inducible one that is activated by L-arabinose and repressed by araC.</p> | <p>The pBAD promoter is a very common inducible one that is activated by L-arabinose and repressed by araC.</p> | ||
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<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | < | + | <p class="montserrat-text uppercase">Terminator (B0010)</p> |
</div> | </div> | ||
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<p>Terminators are necessary to stop the transcription at the end of a coding sequence.</p> | <p>Terminators are necessary to stop the transcription at the end of a coding sequence.</p> | ||
</div> | </div> | ||
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<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | < | + | <p class="montserrat-text uppercase">RBS (B0034)</p> |
</div> | </div> | ||
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<p>Efficient ribosome binding site characterized by IIT Madras in iGEM 2016.</p> | <p>Efficient ribosome binding site characterized by IIT Madras in iGEM 2016.</p> | ||
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<div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | <div class="section-title" style="text-align:left;float:left;width:100%;margin-bottom:0"> | ||
| − | < | + | <p class="montserrat-text uppercase">RFP (E1010)</p> |
</div> | </div> | ||
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<BR><BR> | <BR><BR> | ||
<p>Fluorescent proteins are very common reporter genes. This particular one comes from <i>Discosoma striata</i> and was engineered to be monomeric. The excitation peak is at 584 nm, and the emission peak at 607 nm.</p> | <p>Fluorescent proteins are very common reporter genes. This particular one comes from <i>Discosoma striata</i> and was engineered to be monomeric. The excitation peak is at 584 nm, and the emission peak at 607 nm.</p> | ||
Revision as of 16:26, 1 November 2017
In the lab
Parts we created
ComR (BBa_K2341000)
Protein originally found in Streptococcus thermophilius where it regulates natural competence. The protein activation and binding to specific promoters (ComR Box; BBa_K2341001) is only possible in presence of a short peptide, ComS (LPYFAGCL). Binding to the promoter will induce the downstream sequence expression.
P1655 promoter (BBa_K2341001)
Promote expression of the sequence downstream in presence of ComR (BBa_K2341000) and ComS (LPYFAGCL). Sequence containing the palindrome motif needed to bind ComR (= ComR box).
sgRNA Tyrosin (Cas9 guide) (BBa_K2341100)
RNA guide to use in combination with CRISPR/CAS9. Cut inside the tyrA gene so that deletion by homologous recombination can occur if a homologous DNA strand is also added. RNA guide under constitutive expression by BBa_J23119.
Parts we used
pBAD/araC (I0500)
The pBAD promoter is a very common inducible one that is activated by L-arabinose and repressed by araC.
Terminator (B0010)
Terminators are necessary to stop the transcription at the end of a coding sequence.
RBS (B0034)
Efficient ribosome binding site characterized by IIT Madras in iGEM 2016.
RFP (E1010)
Fluorescent proteins are very common reporter genes. This particular one comes from Discosoma striata and was engineered to be monomeric. The excitation peak is at 584 nm, and the emission peak at 607 nm.
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