Difference between revisions of "Team:UST Beijing/InterLab"

 
(26 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 +
 
<html manifest="cache.manifest" lang="en" dir="ltr">
 
<html manifest="cache.manifest" lang="en" dir="ltr">
 
<head>
 
<head>
Line 38: Line 39:
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/main?action=raw&amp;ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/main?action=raw&amp;ctype=text/javascript"></script>
  
−
<meta name="diaoyong" DanmuPlayer "..//github.com/chiruom/danmuplayer/ - Licensed under the MIT" >
+
<meta name="diaoyong" DanmuPlayer="..//github.com/chiruom/danmuplayer/ - Licensed under the MIT" >
 
<!--[if lt IE 9]>
 
<!--[if lt IE 9]>
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/respond?action=raw&amp;ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/respond?action=raw&amp;ctype=text/javascript"></script>
Line 51: Line 52:
  
 
<!--Hero_Section-->
 
<!--Hero_Section-->
−
<section id="hero_section" class="top_cont_outer">
 
−
  <div class="hero_wrapper">
 
−
    <div class="container">
 
−
      <div class="hero_section">
 
−
        <div class="row">
 
  
−
  <img alt="图标" src="https://static.igem.org/mediawiki/2017/e/ec/T--UST_Beijing--xuu.gif" align="middle">
 
−
        </div>
 
−
      </div>
 
−
    </div>
 
−
  </div>
 
−
</section>
 
−
<!--Hero_Section-->
 
  
−
<!--Header_section-->
+
 
 
<header id="header_wrapper">
 
<header id="header_wrapper">
 
     <div class="header_box">
 
     <div class="header_box">
Line 75: Line 64:
 
    <div id="main-nav" class="collapse navbar-collapse navStyle">
 
    <div id="main-nav" class="collapse navbar-collapse navStyle">
 
<ul class="nav navbar-nav" id="mainNav">
 
<ul class="nav navbar-nav" id="mainNav">
−
  <!--<li class="active">
+
 
−
  <a0 id="ix0" href="#hero_section">
+
  <li>
 +
  <a href="https://2017.igem.org/Team:UST_Beijing/Background">
 
  <span id="ca-icon0"></span>
 
  <span id="ca-icon0"></span>
−
  <div class="scroll-link">Background</div>
+
  <div class="scroll-link">Background</div></a></li>
−
  </a0></li>
+
  <li><a  id="cs2">
−
  <li><a1 id="ix1" href="#aboutUs">
+
 
  <span id="ca-icon1"></span>
 
  <span id="ca-icon1"></span>
−
  <div class="scroll-link">Project</div></a1></li>
+
  <div class="scroll-link">Project</div></a>
−
  <li><a2 id="ix2" href="#experience">
+
  <ul id="csy2">
 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Cyclase">Cyclase</a></li>
 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Experiments">Glucosidase</a></li>
 +
                        </ul>
 +
  </li>
 +
  <li><a  id="cs3" href="https://2017.igem.org/Team:UST_Beijing/Modeling">
 
  <span id="ca-icon2"></span>
 
  <span id="ca-icon2"></span>
−
  <div class="scroll-link">Modeling</div></a2></li>
+
  <div class="scroll-link" >Modeling</div></a>
−
  <li><a3 id="ix3" href="#aboutUs">
+
  <ul id="csy3">
 +
 
 +
                        </ul></li>
 +
  <li><a href="https://2017.igem.org/Team:UST_Beijing">
 
  <span id="ca-icon3"></span>
 
  <span id="ca-icon3"></span>
−
  <div class="scroll-link">HomePage</div></a3></li>
+
  <div class="scroll-link">HomePage</div></a></li>
−
  <li><a4 id="ix4" href="https://2017.igem.org/Team:UST_Beijing/Team.html">
+
  <li><a href="https://2017.igem.org/Team:UST_Beijing/Basic_Part">
 
  <span id="ca-icon4"></span>
 
  <span id="ca-icon4"></span>
−
  <div class="scroll-link">Team</div></a4></li>
+
  <div class="scroll-link" >Parts</div></a></li>
−
  <li><a5 id="ix5" href="#Portfolio">
+
  <li><a href="https://2017.igem.org/Team:UST_Beijing/Team">
 
  <span id="ca-icon5"></span>
 
  <span id="ca-icon5"></span>
−
  <div class="scroll-link">Awards</div></a5></li>
 
−
  <li><a6 id="ix6" href="#service">
 
−
  <span id="ca-icon6"></span>
 
−
  <div class="scroll-link">Notebook</div></a6></li>
 
−
<!--<li><a href="https://2017.igem.org/Team:UST_Beijing/Note.html">
 
−
<span id="ca-icon7"></span><li class="active">--》-->
 
−
<li class="active">
 
−
  <a id="ix0" href="#hero_section">
 
−
  <span id="ca-icon0"></span>
 
−
  <div class="scroll-link">Background</div>
 
−
  </a></li>
 
−
  <li><a href="#aboutUs">
 
−
  <span id="ca-icon1"></span>
 
−
  <div class="scroll-link">Project</div></a></li>
 
−
  <li><a href="#experience">
 
−
  <span id="ca-icon2"></span>
 
−
  <div class="scroll-link">Modeling</div></a></li>
 
−
  <li><a href="#aboutUs">
 
−
  <span id="ca-icon3"></span>
 
−
  <div class="scroll-link">HomePage</div></a></li>
 
−
  <li><a href="https://2017.igem.org/Team:UST_Beijing/Team.html">
 
−
  <span id="ca-icon4"></span>
 
 
  <div class="scroll-link">Team</div></a></li>
 
  <div class="scroll-link">Team</div></a></li>
−
  <li><a href="#Portfolio">
+
  <li><a id="cs5">
−
  <span id="ca-icon5"></span>
+
−
  <div class="scroll-link">Awards</div></a></li>
+
−
  <li><a href="https://2017.igem.org/Team:UST_Beijing/Note.html">
+
 
  <span id="ca-icon6"></span>
 
  <span id="ca-icon6"></span>
−
  <div class="scroll-link">Notebook</div></a></li>
+
  <div class="scroll-link">Awards</div></a>
−
<!--<li><a href="https://2017.igem.org/Team:UST_Beijing/Note.html">
+
<ul id="csy5">
−
<span id="ca-icon7"></span>
+
<li><a href="https://2017.igem.org/Team:UST_Beijing/HP/Silver">HP</a></li><br/>
−
  <div clas s="scroll-link">Modeling</div></a></li>
+
<li><a href="https://2017.igem.org/Team:UST_Beijing/Collaborations">Collaborations</a></li>
−
</ul>
+
<li><a href="https://2017.igem.org/Team:UST_Beijing/Safety">Safety</a></li><br/>
−
  <div class="scroll-link">Modeling</div></a></li>-->
+
    <li><a href="https://2017.igem.org/Team:UST_Beijing/Attributions">Attributions</a></li>
−
 
+
                  <li><a href="https://2017.igem.org/Team:UST_Beijing/InterLab">InterLab</a></li>
−
</ul>
+
                        </ul>
 +
      </li>
 +
  </ul>
 
       </div>
 
       </div>
 
</nav>
 
</nav>
 
     </div>
 
     </div>
 
</header>
 
</header>
−
<!--Header_section-->
 
  
  
  
−
 
−
      <section id="aboutUs"><!--Aboutus-->
 
−
<div class="inner_wrapper aboutUs-container fadeInLeft animated wow">
 
−
  <div class="container">
 
−
    <h2>Wiki of InterLab</h2>
 
−
<h6>by UST_Beijing</h6>
 
  
−
  </div>
+
<section style="background-image:url(https://static.igem.org/mediawiki/2017/f/f8/Ustbbjbj.png);background-position:center center;background-repeat:no-repeat;-moz-background-size: cover;background-size: cover;background-attachment:fixed;">
−
    <div class="inner_section">
+
 
−
  <div class="row">
+
−
<div class="col-lg-12 about-us">
+
−
<div class="row">
+
−
<div class="col-md-6">
+
−
<h3>Background</h3>
+
−
<p>
+
−
“All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader. </p>
+
−
<h3>Materials & Methods</h3>
+
−
                                                                        <h4>Plasmids used</h4>
+
−
<p>
+
−
Kit Plate 7</p>
+
−
<p>Positive Control (BBa_I20270): well 21B</p>
+
−
<p>Negative Control (BBa_R0040): well 21D</p>
+
−
<p>Test Device 1 (BBa_J364000): well 21F</p>
+
−
<p>Test Device 2 (BBa_J364001): well 21H</p>
+
−
<p>Test Device 3 (BBa_J364002): well 21J</p>
+
−
<p>Test Device 4 (BBa_J364003): well 21L</p>
+
−
<p>Test Device 5 (BBa_J364004): well 21N</p>
+
−
<p>Test Device 6 (BBa_J364005): well 21P</p>
+
−
<p>curve by using plate reader. </p>
+
  
−
<ul class="about-us-list">
 
−
<li class="points">A</li>
 
−
<li class="points">B </li>
 
−
<li class="points">C </li>
 
−
                                        <li class="points">D </li>
 
−
<li class="points">E</li>
 
−
</ul><!-- /.about-us-list -->
 
  
−
</div><!-- /.col-md-6 -->
+
<div class="container" align="center">
 +
    <br /><br /><br />
 +
          <h2 style="font-family:Arial">InterLab Measurement Study</h2><br />
 +
          <p class="text-justify" style="width:700px;text-indent:1em; text-align: left;font-size:18px;line-height:28px;font-family:Arial">
 +
          “All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader.
 +
      </p>
 +
        <br /><br /><br /><br /><br /><br />
 +
          <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Plasmids used</p>
 +
          <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
Kit Plate 7 <br />
 +
Positive Control (BBa_I20270): well 21B<br />
 +
Negative Control (BBa_R0040): well 21D<br />
 +
Test Device 1 (BBa_J364000): well 21F<br />
 +
Test Device 2 (BBa_J364001): well 21H<br />
 +
Test Device 3 (BBa_J364002): well 21J<br />
 +
Test Device 4 (BBa_J364003): well 21L<br />
 +
Test Device 5 (BBa_J364004): well 21N<br />
 +
Test Device 6 (BBa_J364005): well 21P<br />
 +
<br />
 +
          </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Strain used</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
        Escherichia coli DH5α<br />
 +
        <br />
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Materials</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
FITC Standard: one tube with dried down FITC for creating a FITC standard <br />
 +
LUDOX: one tube with 30% colloidal silica suspended in 1mL of water <br />
 +
1xPBS (phosphate buffered saline) <br />
 +
LB (Luria Bertani) media <br />
 +
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH) <br />
 +
50 ml Falcon tube (or equivalent) or 250 ml shake flask for cell growth <br />
 +
1.5 ml eppendorf tubes for sample storage <br />
 +
Ice bucket with ice <br />
 +
Pipettes <br />
 +
96 well plate  <br />
 +
<br />
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Machines</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
        Enspire 2300-001M<br />
 +
        Sigma Laborzentrifugen<br />
 +
        Thermo Scientific Nanodrop 2000<br />
 +
        EVOS AMG microscope<br />
 +
        <br/>
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Software</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
        Microsoft Excel<br />
 +
        <br/>
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Methods</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
        Calibration<br />
 +
        OD600 Reference point<br />
 +
        FITC fluorescence standard curve<br />
 +
        Test Device 1 (BBa_J364000): well 21F<br />
 +
        Test Device 2 (BBa_J364001): well 21H<br />
 +
        Test Device 3 (BBa_J364002): well 21J<br />
 +
        Test Device 4 (BBa_J364003): well 21L<br />
 +
        Test Device 5 (BBa_J364004): well 21N<br />
 +
        Test Device 6 (BBa_J364005): well 21P<br />
 +
        <br/>
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Cell measurement</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial">
 +
        Transformation<br />
 +
        Measurements<br />
 +
        <br/>
 +
        </p>
 +
        <br /><br /><br />
 +
        <p  style="width:700px;border-bottom:1px solid black; font-family:Arial;font-size:28px;line-height:50px;text-align: left">Protocols</p>
 +
        <br />
 +
        <p class="text-justify" style="width:700px;font-size:18px;line-height:28px;font-family:Arial" >
 +
        <a href="http://parts.igem.org/Help:Protocols/Transformation" style="color:blue">Please click here</a>
 +
        <br/>
 +
        </p>
 +
        <br /><br /><br /><br /><br /><br />
 +
        <object width="70%" height="800px" data="https://static.igem.org/mediawiki/2017/e/e8/Wiki_of_InterLab_by_UST_Beijing.pdf">  
 +
        </object>
 +
</div>
 +
  <br /><br /><br /><br />
  
−
<div class="col-md-6"> <br/>
+
    <div class="container" align="center">
−
<p>
+
        <p align="center" style="font-size: 5px"><br><h5>Copyright © 2017 UST_Beijing iGEM. All rights reserved.</h5></p>
−
Lorem ipsum dolor sit amet, consectetur Morbi sagittis, sem quisci ipsum gravida tortor orci ipsum gravida tortor. Lorem ipsum dolor sit amet, tortor orci ipsum gravida tortor. Lorem ipsum dolor sit amet, conseconsectetur.
+
    </div>
−
</p><br/><br/>
+
   
−
        <div class="barWrapper">
+
</section>
−
<span class="progressText"><B>k1</B></span>
+
−
<div class="progress">
+
−
  <div class="progress-bar" role="progressbar" aria-valuenow="85" aria-valuemin="0" aria-valuemax="100" >
+
−
<span  class="popOver" data-toggle="tooltip" data-placement="top" title="85%"> </span>
+
−
</div>
+
−
</div>
+
  
−
<div class="barWrapper">
+
</body>
−
<span class="progressText"><B>k2_Vmax</B></span>
+
−
<div class="progress ">
+
−
  <div class="progress-bar" role="progressbar" aria-valuenow="75" aria-valuemin="10" aria-valuemax="100" style="">
+
−
<span  class="popOver" data-toggle="tooltip" data-placement="top" title="75%"> </span>
+
−
  </div>
+
  
−
</div>
+
<style>
−
</div>
+
  ul ul {
 +
    background:#E4EAF1;
 +
    border-radius:0;
 +
    position:absolute;
 +
    top:100%;
 +
    padding:0;
  
−
<div class="barWrapper">
+
}
−
<span class="progressText"><B>k2_Km</B></span>
+
ul  {
−
<div class="progress">
+
    float:none;
−
  <div class="progress-bar" role="progressbar" aria-valuenow="65" aria-valuemin="0" aria-valuemax="100">
+
    border-top:1px solid #6b727c;
−
<span  class="popOver" data-toggle="tooltip" data-placement="top" title="65%"> </span>
+
    border-bottom:1px solid #575f6a;
−
  </div>
+
−
</div>
+
−
</div>
+
−
<div class="barWrapper">
+
−
<span class="progressText"><B>k2_Ki</B></span>
+
−
<div class="progress">
+
−
  <div class="progress-bar" role="progressbar" aria-valuenow="55" aria-valuemin="0" aria-valuemax="100">
+
−
<span  class="popOver" data-toggle="tooltip" data-placement="top" title="55%"> </span>
+
−
  </div>
+
−
</div>
+
−
</div>
+
  
−
</div>
 
−
</div><!-- /.col-md-6 -->
 
−
</div><!-- /.row -->
 
−
</div><!-- /.col-lg-12 -->
 
−
</div>
 
  
−
    </div>
+
}
−
  </div>
+
body{
−
  </div>
+
font-family:Arial;
−
</section>
+
}
−
<!--Aboutus-->
+
.scroll-link{
 +
font-size: 14px;
 +
}
 +
ul ul li a {
 +
    color:#FFF;
  
 +
}
  
−
<!--Footer-->
+
ul ul li a:hover {
−
<footer class="footer_wrapper" id="contact">
+
     background:#FFFFFF;
−
  <div class="container">
+
−
     <section class="page_section contact" id="contact">
+
−
      <div class="contact_section">
+
−
        <h2>Get In Touch</h2>
+
−
<h6>Lorem ipsum dolor sit amet, consectetur Morbi sagittis, sem quisci ipsum</h6>
+
  
−
      </div>
+
}
−
      <div class="row">
+
−
        <div class="col-lg-4 wow fadeInLeft">
+
−
<div class="contact_info">
+
−
                            <div class="detail">
+
−
                                <h4>dsq</h4>
+
−
                                <p>30, xueyuan street, BeiJing, CHINA</p>
+
−
                            </div>
+
−
                            <div class="detail">
+
−
                                <h4>call us</h4>
+
−
                                <p>13051508001</p>
+
−
                            </div>
+
−
                            <div class="detail">
+
−
                                <h4>Email us</h4>
+
−
                                <p>jackplayer0@163.com</p>
+
−
                            </div>
+
−
                        </div>
+
  
 +
ul ul ul {
 +
width: 100%;
 +
position: absolute;
 +
left: 100%;
 +
top: 50%;
 +
}
 +
</style>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy1').hide(); //打开页面隐藏下拉列表
 +
$('#cs1').hover( //鼠标滑过导航栏目时
 +
function(){
 +
$('#csy1').show(); //显示下拉列表
  
 +
},
 +
function(){
 +
$('#csy1').hide(); //鼠标移开后隐藏下拉列表
 +
}
 +
);
 +
$('#csy1').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
function(){
 +
$('#csy1').show();
 +
},
 +
function(){
 +
$('#csy1').hide();
  
−
          <ul class="social_links">
+
}
−
            <li class="twitter animated bounceIn wow delay-02s"><a href="javascript:void(0)"><i class="fa fa-twitter"></i></a></li>
+
);
−
            <li class="facebook animated bounceIn wow delay-03s"><a href="javascript:void(0)"><i class="fa fa-facebook"></i></a></li>
+
$('cs1').hover( //鼠标滑过下拉列表是改变当前栏目样式
−
            <li class="pinterest animated bounceIn wow delay-04s"><a href="javascript:void(0)"><i class="fa fa-pinterest"></i></a></li>
+
);
−
            <li class="gplus animated bounceIn wow delay-05s"><a href="javascript:void(0)"><i class="fa fa-google-plus"></i></a></li>
+
});
−
          </ul>
+
</script>
−
        </div>
+
<script type="text/javascript">
−
        <div class="col-lg-8 wow fadeInLeft delay-06s">
+
$(document).ready(function (){
−
          <!--NOTE: Update your email Id in "contact_me.php" file in order to receive emails from your contact form-->
+
$('#csy2').hide(); //打开页面隐藏下拉列表
 +
$('#cs2').hover( //鼠标滑过导航栏目时
 +
function(){
 +
$('#csy2').show(); //显示下拉列表
 +
 
 +
},
 +
function(){
 +
$('#csy2').hide(); //鼠标移开后隐藏下拉列表
 +
}
 +
);
 +
$('#csy2').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
function(){
 +
$('#csy2').show();
 +
},
 +
function(){
 +
$('#csy2').hide();
 +
 
 +
}
 +
);
 +
$('cs2').hover( //鼠标滑过下拉列表是改变当前栏目样式
 +
);
 +
});
 +
</script>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy3').hide(); //打开页面隐藏下拉列表
 +
$('#cs3').hover( //鼠标滑过导航栏目时
 +
function(){
 +
$('#csy3').show(); //显示下拉列表
 +
 
 +
},
 +
function(){
 +
$('#csy3').hide(); //鼠标移开后隐藏下拉列表
 +
}
 +
);
 +
$('#csy3').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
function(){
 +
$('#csy3').show();
 +
},
 +
function(){
 +
$('#csy3').hide();
 +
 
 +
}
 +
);
 +
$('cs3').hover( //鼠标滑过下拉列表是改变当前栏目样式
 +
);
 +
});
 +
</script>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy4').hide(); //打开页面隐藏下拉列表
 +
$('#cs4').hover( //鼠标滑过导航栏目时
 +
function(){
 +
$('#csy4').show(); //显示下拉列表
 +
 
 +
},
 +
function(){
 +
$('#csy4').hide(); //鼠标移开后隐藏下拉列表
 +
}
 +
);
 +
$('#csy4').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
function(){
 +
$('#csy4').show();
 +
},
 +
function(){
 +
$('#csy4').hide();
 +
 
 +
}
 +
);
 +
$('cs4').hover( //鼠标滑过下拉列表是改变当前栏目样式
 +
);
 +
});
 +
</script>
 +
<script type="text/javascript">
 +
$(document).ready(function (){
 +
$('#csy5').hide(); //打开页面隐藏下拉列表
 +
$('#cs5').hover( //鼠标滑过导航栏目时
 +
function(){
 +
$('#csy5').show(); //显示下拉列表
 +
 
 +
},
 +
function(){
 +
$('#csy5').hide(); //鼠标移开后隐藏下拉列表
 +
}
 +
);
 +
$('#csy5').hover( //鼠标滑过下拉列表自身也要显示，防止无法点击下拉列表
 +
function(){
 +
$('#csy5').show();
 +
},
 +
function(){
 +
$('#csy5').hide();
 +
 
 +
}
 +
);
 +
$('cs5').hover( //鼠标滑过下拉列表是改变当前栏目样式
 +
);
 +
});
 +
</script>
  
−
<form name="sentMessage" id="contactForm"  novalidate>
 
−
<div class="control-group">
 
−
                    <div class="controls">
 
−
<input type="text" class="form-control"
 
−
    placeholder="Full Name" id="name" required
 
−
          data-validation-required-message="Please enter your name" />
 
−
  <p class="help-block"></p>
 
−
  </div>
 
−
        </div>
 
−
                <div class="control-group">
 
−
                  <div class="controls">
 
−
<input type="email" class="form-control" placeholder="Email"
 
−
              id="email" required
 
−
    data-validation-required-message="Please enter your email" />
 
−
</div>
 
−
    </div>
 
  
−
              <div class="control-group">
 
−
                <div class="controls">
 
−
<textarea rows="10" cols="100" class="form-control"
 
−
                      placeholder="Message" id="message" required
 
−
      data-validation-required-message="Please enter your message" minlength="5"
 
−
                      data-validation-minlength-message="Min 5 characters"
 
−
                        maxlength="999" style="resize:none"></textarea>
 
−
</div>
 
−
              </div>
 
−
    <div id="success"> </div> <!-- For success/fail messages -->
 
−
    <button type="submit" class="btn btn-primary pull-right">Send</button><br />
 
−
          </form>
 
−
        </div>
 
−
      </div>
 
−
    </section>
 
−
  </div>
 
−
  <div class="container">
 
−
    <div class="footer_bottom"><span>Copyright © 2018,    Template by <a href="https://webthemez.com/free-bootstrap-templates/">WebThemez.com</a>. </span> </div>
 
−
  </div>
 
−
</footer>
 
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/bootstrapmin?action=raw&amp;ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/bootstrapmin?action=raw&amp;ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/jqueryscrolltofixed?action=raw&amp;ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/jqueryscrolltofixed?action=raw&amp;ctype=text/javascript"></script>
Line 317: Line 399:
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/main?action=raw&amp;ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2017.igem.org/Team:UST_Beijing/js/main?action=raw&amp;ctype=text/javascript"></script>
  
−
<script>
 
−
$("#danmup").DanmuPlayer({
 
−
src: "https://2017.igem.org/File:T--UST_Beijing--xzhufu.mp4",
 
−
    height: "480px", //区域的高度
 
−
    width: "800px" //区域的宽度
 
−
    ,urlToGetDanmu:"getData.php"
 
−
    ,urlToPostDanmu:"sendData.php"
 
−
  });
 
−
 
−
  $("#danmup .danmu-div").danmu("addDanmu",[
 
−
    { "text":"This is so cooool!!!" ,color:"white",size:1,position:0,time:2}
 
−
    ,{ "text":"Ha, I am green" ,color:"green",size:1,position:0,time:3}
 
−
    ,{ "text":"LOL" ,color:"#f30",size:1,position:0,time:10}
 
−
    ,{ "text":"I am yellow gay" ,color:"yellow" ,size:1,position:0,time:3}
 
−
    ,{ "text":"Come on, baby" , color:"red" ,size:1,position:0,time:19}
 
−
    ,{ "text":"Hello, I would glad to be your friend!" ,color:"orange",size:1,position:0,time:23}
 
−
 
−
  ])
 
−
</script>
 
−
</body>
 
−
<!--<link rel="stylesheet" href="css/danmuplayer.css">
 
−
<script src="js/danmuplayer.js"></script>
 
−
<script src="js/lib/jquery-1.11.1.min.js"></script>
 
−
<script>
 
−
  $("#danmup").DanmuPlayer({
 
−
   
 
−
    height: "480px", //区域的高度
 
−
    width: "800px" //区域的宽度
 
−
    ,urlToGetDanmu:"query.php"
 
−
    ,urlToPostDanmu:"stone.php"
 
−
  });
 
−
 
−
  $("#danmup .danmu-div").danmu("addDanmu",[
 
−
    { "text":"这是滚动弹幕" ,color:"white",size:1,position:0,time:2}
 
−
    ,{ "text":"我是帽子绿" ,color:"green",size:1,position:0,time:3}
 
−
    ,{ "text":"哈哈哈啊哈" ,color:"black",size:1,position:0,time:10}
 
−
    ,{ "text":"这是顶部弹幕" ,color:"yellow" ,size:1,position:1,time:3}
 
−
    ,{ "text":"这是底部弹幕" , color:"red" ,size:1,position:2,time:9}
 
−
    ,{ "text":"大家好，我是伊藤橙" ,color:"orange",size:1,position:1,time:3}
 
−
 
−
  ])
 
−
</script>-->
 
  
 
</html>
 
</html>

Latest revision as of 16:59, 1 November 2017

USTB-Beijing | Welcome




InterLab Measurement Study


“All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader.







Plasmids used


Kit Plate 7
Positive Control (BBa_I20270): well 21B
Negative Control (BBa_R0040): well 21D
Test Device 1 (BBa_J364000): well 21F
Test Device 2 (BBa_J364001): well 21H
Test Device 3 (BBa_J364002): well 21J
Test Device 4 (BBa_J364003): well 21L
Test Device 5 (BBa_J364004): well 21N
Test Device 6 (BBa_J364005): well 21P




Strain used


Escherichia coli DH5α




Materials


FITC Standard: one tube with dried down FITC for creating a FITC standard
LUDOX: one tube with 30% colloidal silica suspended in 1mL of water
1xPBS (phosphate buffered saline)
LB (Luria Bertani) media
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH)
50 ml Falcon tube (or equivalent) or 250 ml shake flask for cell growth
1.5 ml eppendorf tubes for sample storage
Ice bucket with ice
Pipettes
96 well plate




Machines


Enspire 2300-001M
Sigma Laborzentrifugen
Thermo Scientific Nanodrop 2000
EVOS AMG microscope




Software


Microsoft Excel




Methods


Calibration
OD600 Reference point
FITC fluorescence standard curve
Test Device 1 (BBa_J364000): well 21F
Test Device 2 (BBa_J364001): well 21H
Test Device 3 (BBa_J364002): well 21J
Test Device 4 (BBa_J364003): well 21L
Test Device 5 (BBa_J364004): well 21N
Test Device 6 (BBa_J364005): well 21P




Cell measurement


Transformation
Measurements




Protocols


Please click here












Copyright © 2017 UST_Beijing iGEM. All rights reserved.